A kind of immobilized horseradish peroxidase carrier and its preparation and application method

A horseradish peroxidase and carrier technology, applied in directions such as being fixed on/in an organic carrier, can solve the problems of unreliable binding, low affinity, environmental pollution, etc., so as to improve operational stability, The effect of stable chemical properties and low cost
CN105039299BActive Publication Date: 2018-03-06ANHUI UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI UNIVERSITY
Publication Date
2018-03-06

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Abstract

The invention belongs to the field of biotechnology, and specifically relates to an immobilized horseradish peroxidase carrier and its preparation and application method, wherein the immobilized horseradish peroxidase carrier is nano-carbon spheres prepared by hydrothermal carbonization of glucose , and then it is obtained by amination modification and glutaraldehyde activation; the immobilized horseradish peroxidase carrier of the present invention is mixed and shaken with the horseradish peroxidase solution, centrifuged, washed and freeze-dried to obtain the immobilized horseradish peroxidase root peroxidase. The invention provides a green, economical and efficient method for preparing immobilized horseradish peroxidase, the immobilized horseradish peroxidase obtained by the method is low in cost, simple in operation, stable in physical and chemical properties, especially in extreme environments High catalytic activity can still be maintained under these conditions.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a preparation method of an immobilized horseradish peroxidase carrier. Background technique

[0002] Horseradish peroxidase is an enzyme composed of glycoprotein and iron porphyrin prosthetic group, which can catalyze the degradation of phenol, aniline and their substitutes, and has a wide range of substrate specificity. Therefore, it has far-reaching application potential in the fields of wastewater treatment, biosensors, environmental monitoring, immunoassays, and clinical chemistry. But because the stability of natural horseradish peroxidase comprises thermal stability, acid-base stability, organic phase stability and storage stability etc. are all relatively poor. Its half-life is about 10 hours at 37°C, and its active half-life is only about 5 minutes at 65°C. The stability is worse in some medium-strong polar organic solvents mixed with water solvents, and free enzymes in water ...

Claims

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