The invention relates to a method for automatically constructing a stable electrocatalytic bacterial
biofilm. The method comprises the following steps: firstly assembling a microbe electrochemical reactor, and connecting circuits, wherein the first circuit is as follows: a fixed
resistor is connected between an
anode and a
cathode in series, and the second circuit is as follows: an adjustable direct-current power supply, an
ampere meter and a work contact of a circulating time
relay are connected between the
cathode and the
anode; then injecting a
bacteria growth medium solution into an
anode chamber, inoculating a
bacteria source, prestarting a microbe anode by switching on the first circuit, then controlling
pulse current between the anode and the
cathode to be gradually increased at preset velocity according to the
potential change of the anode by switching on the adjustable direct-current power supply of the second circuit, wherein the
pulse current is immediately lowered by 40%-60% when the potential of the anode is increased to a
limit value and enters a new round of
growth stimulation, and the electrocatalytic bacterial
biofilm is completely constructed till the
pulse current value reaches a preset current
limit value and is stable at the preset current
limit value for more than 10 hours. The electrocatalytic bacterial
biofilm disclosed by the invention can be stably operated with the relatively high working current.