Human-derived biological tissue material and culture cell stimulation method and device

A technology for biological tissue and cell culture, applied in tissue cell/virus culture devices, biochemical equipment and methods, methods for supporting/immobilizing microorganisms, etc. Problems such as poor material compliance

Pending Publication Date: 2022-03-01
太阳雨林(厦门)生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The first purpose of the present invention is to solve the technical problems of poor compliance of biological tissue materials in the prior art and cannot well adapt to the movement trend of human active parts in view of the above-mentioned defects and deficiencies, and provide a human-derived biological tissue material

Method used

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  • Human-derived biological tissue material and culture cell stimulation method and device
  • Human-derived biological tissue material and culture cell stimulation method and device
  • Human-derived biological tissue material and culture cell stimulation method and device

Examples

Experimental program
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Effect test

Embodiment 1

[0060] A method and device for dynamically cultivating a biocomposite esophagus of a polymer material under stimulation of mechanical properties. The materials of the dense polymer nanofiber layer (i.e. a reinforcement layer 11) and the porous support (i.e. a cell culture layer 20) include polyurethane, polytetrafluoroethylene, expanded polytetrafluoroethylene, silk protein (fibroin ), silk fibroin, polycaprolactone (PCL), polylactic acid (PLA), polyethylene terephthalate, polyglycolic acid (PGA), polylactic-polyglycolic acid (PLGA) , carboxymethyl starch, starch acetate, chitosan (Chitosan), carboxymethyl chitosan, alginic acid / alginate, carboxymethyl cellulose, gelatin, collagen (Ⅰ, Ⅱ, Ⅲ, Ⅳ), transparent At least one of hyaluronic acid (HA), polyvinyl alcohol (PVA), polyacrylamide (PAM), polyacrylic acid, and polyvinylpyrrolidone (PVP).

[0061] Preferably: the thickness of the dense polymer nanofiber layer is 5-200 μm, the diameter of the pores is 1-20 μm, and the fiber di...

Embodiment 2

[0067] A method and equipment for dynamically cultivating an extracellular matrix polymer biocomposite patch under mechanical stimulation.

[0068] The appearance of the extracellular matrix polymer biocomposite patch prepared in this example is as follows: figure 1 Shown, its preparation method is specifically as follows:

[0069] (1) Dissolving PCL and gelatin in hexafluoroisopropanol to obtain an electrospinning stock solution, the specific parameters of which are shown in Table 1 below:

[0070] Table 1 Electrospinning solution preparation parameters

[0071]

[0072] (2) Electrospinning the above-mentioned electrospinning stock solution to obtain such figure 2 The dense polymer nanofiber layer shown; the specific parameters of electrospinning are shown in Table 2 below:

[0073] Table 2 Electrospinning parameter control

[0074]

[0075] (3) Evenly smear the prepared foaming solution on the upper surface of the dense polymer nanofiber layer prepared in step (2...

Embodiment 3

[0085] A method and device for dynamically cultivating biocomposite blood vessels of polymer materials under stimulation of mechanical properties.

[0086] (1) Dissolving PCL and gelatin in hexafluoroisopropanol to obtain an electrospinning stock solution, the specific parameters of which are shown in Table 1 below:

[0087] Table 1 Electrospinning solution preparation parameters

[0088]

[0089]

[0090] (2) Electrospinning the above-mentioned electrospinning stock solution to obtain a dense polymer nanofiber layer, such as Figure 4 , where A is the unbent state, B is the bent state, it can be seen that the tubular structure is still maintained in the bent state,

[0091] The specific parameters of electrospinning are shown in Table 3 below:

[0092] Table 3 Electrospinning parameter control

[0093]

[0094] (3) Evenly smear prepared foaming liquid (polyurethane, polytetrafluoroethylene, expanded polytetrafluoroethylene, silk protein ( fibroin ), silk fibroin...

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Abstract

The invention provides a human-derived biological tissue material and a dynamic culture cell stimulation method and device. The biological tissue material comprises an in-vitro culture matrix and a plurality of moldable cavities capable of accommodating cells to be cultured. In the culture process, the cells are placed in a moldable cavity to secrete an extracellular matrix (ECM). The dynamic culture method comprises the following steps: placing cells in the moldable cavity, and applying different external forces to the cells at different time points to stimulate the cells to secrete ECM. According to different to-be-implanted parts, mechanical forces in different directions, sizes and angles are selected to stimulate cells, so that the cells secrete more ECM and play a shaping role, and a composite biological material of'biological material + shaping cell + ECM 'is formed. The composite biological material becomes an organic integrated body through mechanical stimulation, so that the use of a chemical cross-linking agent is avoided. Cell components (immune components) in the composite material can be removed. The obtained biological tissue materials comprise blood vessels, hernia patches, tendons, cartilages, muscles and the like.

Description

technical field [0001] The present invention relates to the technical field of biological tissue materials, more specifically, to a human-derived biological tissue material and a method and device for stimulating cultured cells. Background technique [0002] The main disadvantages of currently widely used biomaterials such as artificial blood vessels and hernia patches are: (1) The artificial compliance is poor, and it does not have the flexibility and elasticity of human arteries. Obviously, this is also the main reason why thrombus is easy to form at the anastomotic site, leading to thrombus formation and intimal hyperplasia, and the long-term patency rate is extremely low; (2) Hernia mesh is prone to infection, scar, discomfort, local effusion, The formation of the fibrous envelope needs to be taken out if infected; (3) common natural materials require chemical cross-linking agents to forcibly connect the molecules together, otherwise they will be rapidly degraded in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/22A61L27/50A61L27/56C12M3/00C12M3/04
CPCA61L27/3633A61L27/3691A61L27/3687A61L27/18A61L27/222A61L27/50A61L27/507A61L27/56C12M21/08C12M23/20C12M23/48C12M23/58C12M25/14C12M35/04A61L2430/40A61L2400/04A61L2400/12C08L67/04
Inventor 刘弘毅赵一麟郭鹏周媛媛周旭
Owner 太阳雨林(厦门)生物医药有限公司
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