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Solidified tissue immunological adjuvant

An immune adjuvant and tissue technology, which can be used in drug combinations, medical preparations containing active ingredients, bacterial antigen components, etc., and can solve problems such as inappropriate organisms

Inactive Publication Date: 2005-06-01
CELL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the described synthetic polymer poly(DL-lactide co-glycolide) (poly(DL-latide co-glycolide), PLG) produces lactic acid when decomposed, and acidifies the environment of the decomposed area. still inappropriate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0044] Example 1: Stimulation effect of human peripheral blood adherent cells by an insolubilized adjuvant of BCG soluble components (formalin-fixed liver cancer tissue fragments immobilized with ethanol-soluble components derived from BCG bacteria)

[0045] 1. Fragmentation and Washing of Formalin-Fixed Human Hepatoma Tissue

[0046] Human liver cancer tissues were fixed by immersing them in a commercially available neutral formalin solution for 3 days or more at room temperature. After the tissue was taken out, it was cut into fine fragments with a width of 1 mm using ophthalmic scissors, and PBS (+) in an amount 3-10 times the wet weight of the original liver cancer was added, and then passed through a homogenizer while cooling with ice water ( (Baiyi's Rufu) FIAX-600 manufactured by Haidolf Co., Ltd., (Zeneleta Shaft Type 10F) was homogenized for 30 seconds. This homogenization was repeated several times at intervals of 30 seconds or more for ice-water cooling. Take 1.2 ...

example 2

[0078] Example 2: Comparison of the stimulating effects of BCG soluble components and insoluble adjuvants of BCG soluble components on human peripheral blood adherent cells

[0079] According to the method of Example 1, fragmentation and washing of formalin-fixed human liver cancer tissue were carried out, ethanol-soluble components from BCG cells were prepared, insoluble adjuvants of BCG soluble components were prepared, LPS for positive control was prepared, and human peripheral Blood adherent cells, the stimulation of human peripheral blood adherent cells by BCG soluble components and insoluble adjuvants, and the determination of the stimulating effect of adherent cells. Here, while the coated sheet fragments of the BCG soluble fraction were suspended in the culture solution, 2 µl of the BCG extraction solution was added per well in a 96-well plate for culture. For comparison, 2 [mu]l of ethanol per well was also added to the other wells.

[0080] The results are shown in ...

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Abstract

An immunological adjuvant having a potent cell-stimulation effect and a high safety to living bodies, being prepared from a solidified material selected from the group consisting of tissues, cells and components thereof of animals involving humans, containing fragments from which soluble components have been removed by washing with an organic solvent and / or hot water, and having microorganism-origin soluble components immobilized in the fragments.

Description

technical field [0001] The present invention relates to a method for producing a solidified tissue immune adjuvant, which is a method for enhancing general immune response, and a method useful for prevention of recurrence, inhibition of metastasis and treatment of tumors with anti-tumor immune response. Background technique [0002] Although various immune adjuvants have been known in the past, they can enhance the immune response to the antigen when they are administered to the organism at the same time as the antigen, but almost all of the immune adjuvants cause an inflammatory response, and make the immune activity assembled to the inflammatory reaction area Cell activation. In recent years, it has been known that among immunocompetent cells, antigen-presenting cells play the role of the center of immune response, and the most powerful cells are dendritic cells (Dentdritic Cells, second edition, ed.By lotze, M.T. and Thomson , A.W. Academic Press, San Diego, 2001). It i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/02A61K39/39A61P35/00
CPCA61K39/39A61K2039/55588A61K2039/55594A61P35/00
Inventor 大野忠夫内村英次
Owner CELL MEDICINE
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