Ultrasonic microbubble cell stimulation device and operation method thereof

Abstract

The invention belongs to the field of a medical instrument, and provides a ultrasonic microbubble cell stimulation device and an operation method thereof. The ultrasonic microbubble cell stimulation device comprises: a culture dish, a positioning frame, and a base, a culture hole is arranged in the culture dish, a sealing film is arranged on an open end, a culture cup is arranged in the culture hole, and the base is connected with a transmission componentthat is for transmitting an ultrasonic wave to stimulate a cell and is capable of adjusting a distance from the base to the culture dish; andthe base and the positioning frame are connected through a position-adjusting component. The operation method of the ultrasonic microbubble cell stimulation device comprises: a guide rod installationstep; a transmission component connecting and assembly step; a positioning frame installation step; a culture cup installation and microbubble injecting step; and a culture dish installation step. The ultrasonic microbubble cell stimulation device can successfully prevent air bubbles from entering into the culture dish, ensuring that the ultrasonic waves act on the microbubbles directly, has a controllable stimulation area and intensity, is easy to operate, is versatile, and has a wide range of application.

Description

technical field [0001] The invention belongs to the field of medical devices, in particular to an ultrasonic microbubble stimulating cell device and an operation method thereof. Background technique [0002] With the development of science and technology, the cognition of ultrasound diagnosis and treatment is deepening, and the research of ultrasound stimulation has become a hot spot in medical treatment. Ultrasound stimulation has received more and more attention due to its safety, non-invasiveness, effectiveness and real-time performance. Nowadays, many laboratories in the world are conducting research on ultrasonic stimulation of cells, and ultrasonic stimulation microbubbles (or drug-loaded microbubbles) act on cells or lesions to achieve more ideal effects. However, since the microbubbles need to act on the cells at the bottom of the culture dish, the culture dish must be turned upside down to allow the microbubbles to float to the cells at the bottom of the culture di...

AI Technical Summary

Problems solved by technology

However, since the microbubbles need to act on the cells at the bottom of the culture dish, the culture dish must be turned upside down to allow the microbubbles to float to the cells at the bottom of the culture dish, and because air will enter the culture dish when the parafilm seals the culture dish and turns it upside down, it cannot be used. Ultrasound acts on microbubbles and stimulates cells

Images