2results about How to "Way fast" patented technology

This application discloses a technique for detecting target nucleic acid molecules using an activated fluorescent RNA probe, its expression vector, and a detection method. It includes: probe design and preparation methods; labeling and detection of target nucleic acid molecules in solution samples using the probe; labeling and detection of target nucleic acid molecules in living cells using the probe; and labeling and detection of target nucleic acid molecules in immobilized cells using the probe. This invention overcomes the shortcomings of current commercially synthesized FISH and molecular beacon probes, such as their inability to be genetically encoded, difficult preparation, and high cost. It provides an efficient, simple, and rapid method to obtain activated fluorescent RNA probes for detecting target nucleic acid molecules through genetic encoding or in vitro transcription. This offers a practical tool for research on fundamental life science issues and for point-of-care and even real-time diagnosis of diseases, possessing broad scientific and social significance.

The invention discloses a steel structure plate butt welding device and a working method thereof. The steel structure plate butt welding device comprises a bottom plate, a top plate and lateral supports on the two sides. A clamping assembly used for clamping a steel structure plate is arranged on the lateral support, the welding assembly comprises a welding gun, a motor, a third lead screw, a sliding block and an electric push rod, and front-back movement and height adjustment of the welding gun are achieved; the driving assembly comprises a gear, a rotating rod, a rotating disc and a rack, and vertical displacement of the clamping assembly is achieved through gear and rack transmission. A positioning device is further arranged on the rack, comprises a second lead screw and an internal thread knob and is used for finely adjusting and locking the position of the clamping assembly. Coarse adjustment is achieved through the driving assembly, fine adjustment is achieved through the positioning device, the tops of the two plates with different thicknesses are adjusted to the same horizontal plane, it is ensured that welding faces are aligned, the problems that in the prior art, the welding faces are uneven and the welding seam quality is poor due to the plate thickness difference are solved, and the beneficial effects of being convenient and fast to operate, high in welding precision and high in adaptability are achieved.