Methods for culturing haematococcus pluvialis and producing astaxanthin

Abstract

The invention provides a haematococcus pluvialis culture production method and a method for further producing astaxanthin. The haematococcus pluvialis culture process is carried out in a large-size container, and haematococcus pluvialis is cultured under the irradiation of an artificial LED (light-emitting diode) light source; the artificial LED light source is provided by a high-brightness LED, the wavelength range of single-color light emitted by the LED light source is from 450nm (width band of 30nm) to 640nm (width band of 30nm); the used illumination intensity is 30mu E / m<2>.s-3000mu E / m<2>.s; in the culture process, cells are maintained to be suspended in the culture liquid in utilizing a ventilation mode, and temperature is maintained to be 15-28 DEG C; and the pH value is maintained to be 6.8-8.5. According to the invention, on one hand, more nutrient substances are accumulated so as to accelerate growth and reproduction; on the other hand, the problem that the temperature is difficult to control due to the adoption of a natural sun light source is solved, thereby saving a large amount of energy consumed for controlling the temperature in summer and winter, reducing production cost and environmental pollution; and simultaneously, according to the characteristic that photosynthesis pigments demand different light qualities, the most effective single-color diode light source is adopted, thereby improving energy utilization rate, production efficiency and cell concentration.

Description

technical field [0001] The invention relates to a method for cultivating Haematococcus pluvialls on an industrial scale, and also relates to a method for producing astaxanthin on an industrial scale. The Haematococcus pluvialis belongs to freshwater algae. Background technique [0002] Haematococcus pluvialis, also known as Haematococcus pluvialis or Haematococcus lacustrine, belongs to Volvox, Haematococcus family. [0003] In 1899, it was discovered that this algae is often attached to water tanks or shallow water bays near the sea in the form of a blood-red shell. The life course of this algae goes through a red dormant stage, followed by A green swimming phase followed by a red dormant phase. [0004] In 1934, Elliot added details of the growth history of this alga from the point of view of cell morphology. Four typical cell shapes appear throughout the life cycle: small worm body, large worm body with flagella, gelatinous sheath body with no motility, and red large c...

AI Technical Summary

Problems solved by technology

[0010] Therefore, the cultivation of Haematococcus pluvialis is relatively difficult, and the cultivation conditions such as temperature and light are very demanding

Most of the natural lighting methods used in current scientific research and production are difficult to achieve precise temperature control

Especially in summer and winter, a lot of energy is wasted in cooling in summer and heating in winter