Methods for culturing haematococcus pluvialis and producing astaxanthin

A technology of Haematococcus pluvialis and production method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, bulk chemical production, etc. Difficulty in cultivating algae and other problems, to avoid the difficulty of temperature control, save energy, and improve energy utilization.

Inactive Publication Date: 2012-02-01
烟台华融生物科技有限公司
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Problems solved by technology

[0010] Therefore, the cultivation of Haematococcus pluvialis is relatively difficult, and the cultivation conditions such as temperature and light are very demanding
Most of the natural lighting methods u

Method used

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with the accompanying drawings and examples.

[0030] The method for cultivating Haematococcus pluvialls of the present invention comprises the following steps:

[0031] The cultivation process is carried out in a large container of more than 500 liters,

[0032] The first step, reactor sterilization: use chemical or steam method to sterilize the reactor.

[0033] The second step, selecting medium: adopt GB11 medium.

[0034] The third step, medium sterilization: carry out hypochlorous acid chemical sterilization or high temperature sterilization on the medium. After chemical sterilization, the medium is neutralized to pH 6.0-8.5, and after high-temperature sterilization, the medium is cooled to below 28°C.

[0035] The fourth step, inoculation and cultivation: inoculate the green swimming cells of Haematococcus pluvialis into the culture medium, and aerate and cultivate.

[0036] The cultivation p...

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Abstract

The invention provides a haematococcus pluvialis culture production method and a method for further producing astaxanthin. The haematococcus pluvialis culture process is carried out in a large-size container, and haematococcus pluvialis is cultured under the irradiation of an artificial LED (light-emitting diode) light source; the artificial LED light source is provided by a high-brightness LED, the wavelength range of single-color light emitted by the LED light source is from 450nm (width band of 30nm) to 640nm (width band of 30nm); the used illumination intensity is 30mu E/m<2>.s-3000mu E/m<2>.s; in the culture process, cells are maintained to be suspended in the culture liquid in utilizing a ventilation mode, and temperature is maintained to be 15-28 DEG C; and the pH value is maintained to be 6.8-8.5. According to the invention, on one hand, more nutrient substances are accumulated so as to accelerate growth and reproduction; on the other hand, the problem that the temperature is difficult to control due to the adoption of a natural sun light source is solved, thereby saving a large amount of energy consumed for controlling the temperature in summer and winter, reducing production cost and environmental pollution; and simultaneously, according to the characteristic that photosynthesis pigments demand different light qualities, the most effective single-color diode light source is adopted, thereby improving energy utilization rate, production efficiency and cell concentration.

Description

technical field [0001] The invention relates to a method for cultivating Haematococcus pluvialls on an industrial scale, and also relates to a method for producing astaxanthin on an industrial scale. The Haematococcus pluvialis belongs to freshwater algae. Background technique [0002] Haematococcus pluvialis, also known as Haematococcus pluvialis or Haematococcus lacustrine, belongs to Volvox, Haematococcus family. [0003] In 1899, it was discovered that this algae is often attached to water tanks or shallow water bays near the sea in the form of a blood-red shell. The life course of this algae goes through a red dormant stage, followed by A green swimming phase followed by a red dormant phase. [0004] In 1934, Elliot added details of the growth history of this alga from the point of view of cell morphology. Four typical cell shapes appear throughout the life cycle: small worm body, large worm body with flagella, gelatinous sheath body with no motility, and red large c...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCY02P20/54
Inventor 陈勇邹宁
Owner 烟台华融生物科技有限公司
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