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Crypthecodinium connii fermenting process for producing docosahexaenoic acid grease

A technology of docosahexaenoic acid and Cryptidium koirii, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low yield of docosahexaenoic acid and cultivation process Problems such as failure to achieve ideals, to achieve the effect of increasing production

Inactive Publication Date: 2007-06-27
湖北友芝友生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defective that above-mentioned two kinds of methods exist is that the output of docosahexaenoic acid (DHA) is not high
Over the years, many researchers at home and abroad have conducted a large number of researches to screen different strains, and the researches have not obtained ideal results by using various culture techniques.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Medium formula (g / 100ml) (I)

[0031] Sodium chloride 1.25g Potassium chloride 0.07g Calcium chloride 0.035g

[0032] Magnesium sulfate 0.8g Potassium dihydrogen phosphate 0.2g Sodium glutamate 4.0g

[0033] Yeast extract 2.0g glucose 7.5g vitamin B 1 0.003g

[0034] Vitamin B 6 0.005g.

[0035] Add water to the required volume and control the pH at 6.5-7.0.

[0036] Medium (II):

[0037] Relative to the medium (I), the modified medium formed in the medium (I) was supplemented with 10% (V / V) glucose and 2.4% (V / V) yeast extract.

[0038] In the present invention, slant strains are used for liquid shake flask culture, liquid shake flask expansion culture, primary seed culture, secondary seed culture and tertiary fermentation culture to obtain seaweed cells. The specific steps of its production method are as follows:

[0039] The Crypthecodinium cohnii (Seligo) Javornicky cells were inoculated in a sterile slant medium at a temperature of 28°C and a culture time of...

Embodiment 2

[0064] The culture and fermentation of seaweed cells are the same as in Example 1. The extraction method is to add sodium lauryl sulfate as a wall breaker to the concentrated fermentation broth after the fermentation broth flowing out of the fermentor is filtered and concentrated, and the amount is 0.1-0.6% of the weight of the concentrated fermentation broth, and then use After 18h-28h leaching with n-hexane, crude docosahexaenoic acid oil is obtained. After degumming, deacidification, decolorization and deodorization of docosahexaenoic acid crude oil, refined docosahexaenoic acid oil is obtained. .

[0065] DHA refined oil has a peroxide value of 0.1meq / kg and an acid value of 0.14mg(KOH) / g.

[0066] DHA grease is methyl esterified, and its content is determined by gas chromatography analysis.

[0067] The composition of fatty acids in algal cells determined by gas chromatography is as follows.

[0068] Fatty acid name Fatty acid content

[0069] C10:0 0.3532

[0070] C12:0 0.10...

Embodiment 3

[0083] Medium (I)

[0084] Sodium chloride 1.35g Potassium chloride 0.065g Calcium chloride 0.04g

[0085] Magnesium sulfate 0.7g Potassium dihydrogen phosphate 0.3g Sodium glutamate 4.3g

[0086] Yeast extract 3.0g glucose 8.5g vitamin B 1 0.005g

[0087] Vitamin B 6 0.006g

[0088] Add water to 100ml

[0089] Medium (II)

[0090] Relative to the medium (I), the modified medium formed in the medium (I) was supplemented with 10% (V / V) glucose and 2.4% (V / V) yeast extract.

[0091] The cells named Crypthecodinium cohnii (Seligo) Javornicky were inoculated in a sterile slant medium with a culture temperature of 28.5°C and a culture time of 48 hours.

[0092] Put the fresh slant strains into a 500ml Erlenmeyer flask containing 100ml of culture medium, culture on a shaker at 28.5°C for 48-72 hours, and rotate the shaker at 140r / min. Then, 100 ml of the obtained shake flask strains were inoculated into 5 triangular shake flasks (1000 ml), each containing 200 ml medium (pH 6.8) for expa...

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PUM

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Abstract

The present invention is process of fermenting Crypthecodinium cohnii(Seligo)Javornicky as one kind of marine micro algae to produce docosahexaenoic acid grease. The process includes continuous culturing Crypthecodinium cohnii(Seligo)Javornicky in liquid culture medium to obtain algae cell and extracting the algae cell to obtain docosahexaenoic acid grease. The present invention has proper marine micro algae strain, vitamins added into the culture medium and proper technological process, so that the present invention has high yield of docosahexaenoic acid grease, docosahexaenoic acid content in algae cell of 30-50 %, algae cell concentration of 20-40 g / L and oil content in algae cell of 20-50 %.

Description

Technical field [0001] The invention relates to a method for producing polyunsaturated fatty acid docosahexaenoic acid (DHA) oil, specifically a method for fermentation with Crypthecodinium cohnii (Selgo) Javornicky in marine microalgae Method for producing docosahexaenoic acid grease. Background technique [0002] In recent years, the important role of polyunsaturated fatty acids (PUFAs) on human health has received increasing attention. Among them, docosahexaenoic acid is favored by people because of its important physiological functions in humans and animals. Studies have shown that DHA is a basic component of cell membranes in certain tissues of the human body; it plays an important role in the development of the visual system and nervous system of infants and young children; it also has cholesterol-lowering effects, anti-coagulation effects and cancer-inhibiting effects, preventing Alzheimer's disease It is a kind of fatty acid necessary for the growth of juvenile fish. Ther...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/12C12R1/89
Inventor 蔡立刚袁谦简晓红
Owner 湖北友芝友生物科技有限公司
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