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Application of lactobacillus johnsonii in lowering pH value of intestinal tract of weaning piglet

A Lactobacillus johnsonii, weaned piglet technology, applied in the application, animal feed, animal feed, etc.

Inactive Publication Date: 2012-10-17
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no product in China that uses Lactobacillus johnsonii as an additive to add food and feed additives, and its application in animal and poultry breeding is rarely reported. Lactobacillus johnsonii is developed into an animal microecological feed additive. Filling in the domestic gap and screening Lactobacillus johnsonii with excellent probiotic properties is of great significance to promoting the level of livestock and poultry breeding in my country, and can play a greater role in livestock and poultry breeding

Method used

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  • Application of lactobacillus johnsonii in lowering pH value of intestinal tract of weaning piglet
  • Application of lactobacillus johnsonii in lowering pH value of intestinal tract of weaning piglet
  • Application of lactobacillus johnsonii in lowering pH value of intestinal tract of weaning piglet

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Embodiment 1

[0034] The following examples are used to illustrate the present invention, but not to limit the scope of the present invention. Example 1 Preparation of Lactobacillus johnsonii CGMCC No. 4926 lyophilized agent

[0035] 1) Activate the cryopreserved Lactobacillus johnsonii CGMCC No. 4926 on the MRS agar plate three times, pick a single colony and inoculate it in a 10 mL deep MRS liquid test tube, incubate at 37°C for 10 hours, and transfer it to 250 mL MRS at 1% Liquid medium, used as seed solution after 10 hours of cultivation;

[0036] 2) Inoculate the seed liquid obtained in step 1) in a 5L fermentor with an inoculum of 5%;

[0037] 3) Maintain the pH of the fermentation broth at 6.0 during the fermentation process. When the pH exceeds the set value, add the feed solution; when the pH is lower than the set value, automatically add alkaline neutralizer ammonia; at a temperature of 37°C and a rotation speed Fermented at 100rpm for 14 hours, and N 2 , Maintain dissolved oxygen less...

Embodiment 2

[0042] (1) Experimental animals and experimental design

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Abstract

The invention discloses an application of lactobacillus johnsonii CGMCC No.4926 in lowering a pH value of intestinal tract of a weaning piglet. Addition of proper dosage of the lactobacillus johnsonii to forage can lower pH values of ileum and cecum of the weaning piglet. A low pH value of the intestinal tract is not benefit for growth of intestinal tract pathogen, so that with the addition of the lactobacillus johnsonii, effect of prevention of diseases caused by the pathogen can be reached.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of Lactobacillus johnsonii in reducing the pH value of the intestinal tract of weaned piglets. Background technique [0002] Lactobacillus johnsonii belongs to Firmicutes (Firmicutes) Bacilli (Bacilli) Lactobacillales (Lactobacillales) Lactobacillaceae (Lactobacillaceae) Lactobacillus (Lactobacillus), first isolated in 1980, but in the classification It has been classified as Lactobacillus acidophilus in science. In 1992, Fujisawa et al. named it Lactobacillus johnsonii through biochemical experiments and DNA hybridization experiments. Lactobacillus johnsonii mainly exists in the digestive tract of animals and humans, and is the dominant flora. [0003] The European Food Safety Authority (EFSA) will issue two health declarations against Lactobacillus yoselii in 2009 and 2011 respectively, stating that Lactobacillus yoselii has microorganisms that reduce pathogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16
Inventor 张军汪攀孙晓雯闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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