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Application of codonopsis pilosula polysaccharide as medicine for regulating intestinal flora of human body

A technology of Codonopsis polysaccharide and intestinal flora, applied in drug combination, food science, digestive system, etc., can solve the problems of difficult mutual mechanism and unclear interaction relationship.

Inactive Publication Date: 2019-05-14
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in long-term clinical application, Codonopsis Codonopsis is mainly prepared in the form of water decoction and administered orally; Unknown components), and the interaction between the components is not yet clear; it brings difficulties to find the components that play an effective role in the treatment of diseases, and to track the interaction mechanism between effective components

Method used

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  • Application of codonopsis pilosula polysaccharide as medicine for regulating intestinal flora of human body
  • Application of codonopsis pilosula polysaccharide as medicine for regulating intestinal flora of human body
  • Application of codonopsis pilosula polysaccharide as medicine for regulating intestinal flora of human body

Examples

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Effect test

experiment example 1

[0043] Such as figure 1 It has been shown that the establishment of suitable animal models is the key to exploring the pathogenesis of diseases and evaluating the therapeutic effect of new clinical drugs. For IBD, establishing a mouse colitis model is the most commonly used method to explore its pathological mechanism. Common colitis models include: spontaneous, genetically modified, and induced colitis models. Among them, the acute mouse colitis model stimulated by the chemical substance dextran sodium sulfate (DSS) is currently the most widely used. Therefore, this chapter explores the experimental cycle of DSS modeling to prepare for later experiments. Material

[0044] Experimental animals: clean grade C57BL / 6 female mice (18 ± 2g; 4 weeks old) purchased from Hunan Slake Jingda Experimental Animal Co., Ltd.;

[0045] Instruments: NC-MS electronic balance, HMS-350 vortex oscillator, DH-500 constant temperature incubator, HS-202 paraffin slicer;

[0046] Reagent: DSS (m...

experiment example 2

[0065] Experimental Example 2 Study on the effect of drugs on intestinal flora and Th17 / Treg in mice with colitis

[0066] In recent years, with the idea that "multi-components of traditional Chinese medicine act on multiple targets at the same time", restoring the disordered intestinal flora and reshaping the Th17 / Treg balance has become a research hotspot as a new research target for the treatment of IBD . Because of its wide range of pharmacological effects and significant advantages in immune regulation, polysaccharides from traditional Chinese medicines can be degraded by intestinal flora through various mechanisms and then utilized by the body. Codonopsis Codonopsis is a classic traditional Chinese medicine for invigorating the middle and nourishing Qi. The effects of its representative active pharmacological molecule Codonopsis polysaccharide on the intestinal flora of IBD and the expression of cytokines related to Th17 / Treg balance have not yet been clarified. Based o...

experiment example 3

[0119] Effect of Experimental Example 3 Drugs on SCFAs Content and Related Bacteria in Colitis Mice

[0120] As a source of energy for colonic cells, SCFAs can exert anti-inflammatory and antibacterial effects by improving intestinal permeability and internal environment, regulating body energy homeostasis and immune cell chemotaxis. In order to explore whether Codonopsis polysaccharides will affect the changes of SCFAs-producing bacteria in the intestinal tract of colitis mice and the SCFAs content in feces after acting on acute colitis. The present invention detects the content of SCFAs in the feces of mice with acute colitis induced by DSS by gas chromatography (GC), and analyzes the changes of bacterial genera associated with the production of SCFAs in the intestinal tract of colitis mice in combination with the results of 16SrRNA sequencing.

[0121] Experimental Materials

[0122] Instrument: Agilent 7890B gas chromatograph (including FID detector); NC-MS electronic bal...

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Abstract

The invention provides an application of codonopsis pilosula polysaccharide as medicine for regulating intestinal flora of human body, namely the application of codonopsis pilosula polysaccharide as medicine, food or health products for preventing colitis. An acute colitis mouse model is established by using fructo-oligosaccharide, codonopsis pilosula polysaccharide and codonopsis pilosula saponinto be respectively prevented in a healthy mouse model for 21 days and adopting 3% dextran sodium sulfate to be induced for 7 days. The remission of three drugs to macroscopical pathological symptomsof colitis is compared by evaluating changes of body weight, colon length, pathological index of disease, histological section and myeloperoxidase activity of experimental mice and the like. The regulation of drug on intestinal flora of colitis mice is investigated by using the Illumina MiSeq 250 sequencing platform based on the variable region of 16S V3-V4, and dominant bacteria in each drug intervention group are identified, which shows the absorption of the highest exposure amount of monomer saponin in codonopsis pilosula saponin in serum and the cecum, clarifies the mechanism that codonopsis pilosula polysaccharide plays a role in the treatment of diseases by changing the action of intestinal flora on saponin metabolism.

Description

technical field [0001] The invention relates to the field of regulation of codonopsis polysaccharides on intestinal flora, in particular to the application of codonopsis polysaccharides as a medicine for regulating human intestinal flora. Background technique [0002] Gut flora, as an essential part of the human gastrointestinal tract, is a complex community of 100 trillion archaea and bacteria, representing more than 1,000 species, of which more than 90% of the species belong to the Bacteroidetes, thick Microbiota and Actinobacteria. A large number of human and rodent experiments have proved that the intestinal flora is an indispensable "organ" for maintaining the health of the host. [0003] Inflammatory bowel disease (Inflammatory Bowel Disease, IBD) is a type of autoimmune disease mediated by diet, infection, metabolism, susceptibility genes, immunity, intestinal flora and other factors. A persistent, recurrent, and uncontrollable inflammatory response in the intestina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P1/00A23L33/125
Inventor 张春江景亚萍魏俊淑赵唐扎拉加白妍睿李玉玺
Owner LANZHOU UNIVERSITY
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