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A method for identifying chicken microRNAs associated with Campylobacter jejuni infection

A technology of Campylobacter jejuni and identification method, applied in the field of identification of chicken microRNA related to Campylobacter jejuni infection, can solve the problems of no identification of Campylobacter jejuni infection-related microRNA, no identification of microRNA, etc.

Inactive Publication Date: 2016-06-15
SHANDONG AGRICULTURAL UNIVERSITY
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  • Claims
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Problems solved by technology

[0005] At present, there are no studies on the identification of microRNAs associated with Campylobacter jejuni infection, and there is no research on the identification of microRNAs associated with Campylobacter jejuni infection

Method used

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  • A method for identifying chicken microRNAs associated with Campylobacter jejuni infection
  • A method for identifying chicken microRNAs associated with Campylobacter jejuni infection
  • A method for identifying chicken microRNAs associated with Campylobacter jejuni infection

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Embodiment Construction

[0039] In order to make the technical problems, technical solutions and beneficial effects solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0040] Such as Figure 1 to Figure 4 A method for identifying chicken microRNA associated with Campylobacter jejuni infection includes a method for identifying chicken microRNA associated with Campylobacter jejuni infection, including the following steps:

[0041] In the first step, the meconium of 1-day-old chickens is collected, and the positive rate of Campylobacter jejuni is detected through a specific medium;

[0042] In the second step, approximately 10 8 cfu Campylobacter jejuni, the control group was inoculated with the same amount of PBS, and the cecum contents ...

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Abstract

The invention discloses an identification method for campylobacter jejuni infection-associated chicken microRNA. The identification method comprises the following steps of: a first step of collecting chicken meconium, and detecting a positive rate of campylobacter jejuni; a second step of collecting cecum content for counting campylobacter jejuni and cecum tissue samples for RNA extraction; a third step of extracting total RNA of samples, detecting quality, and mixing in a tank; a fourth step of constructing a sequencing sample library; a fifth step of carrying out Solexa sequencing; a sixth step of carrying out subsequent analysis; a seventh step of predicating a target gene of the microRNA; an eighth step of obtaining campylobacter jejuni cfu infection-assocaited chicken microRNAs according to the microRNA and the target gene information thereof. The identification method disclosed by the invention regulates the target gene of the microRNA to indirectly participate in a plurality of immune-associated signal channels, so that important regulation effect is achieved in campylobacter jejuni infection; the microRNAs can be important candidate microRNAs in anti-campylobacter jejuni infection breeding, and foundation is laid up for deeply researching an acting mechanism of the microRNAs in campylobacter jejuni in future, so that theoretical basis and scientific basis are provided for molecular disease-resistant breeding of chicken.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for identifying chicken microRNA associated with Campylobacter jejuni infection. Background technique [0002] Campylobacter jejuni, an important food-borne pathogen, causes disease in livestock and poultry, and causes acute enteritis in humans, which has brought serious economic losses and food safety problems to humans. The cecum is its main reproductive site. The genetic background of the host plays an important role in the chicken's defense against Campylobacter jejuni, and the susceptibility / resistance of different strains (breeds) of chickens to Campylobacter jejuni is significantly different. [0003] microRNA (miRNA) is a kind of endogenous non-coding small RNA with a length of about 22 nt. It inhibits the translation of the target gene or cuts the target gene by combining with the 3'UTR of the target gene, and plays a role in many biological processes such as immuni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/6869C12Q1/689C12Q2600/178C12Q2535/122
Inventor 李显耀刘肖意刘丽英张懋志
Owner SHANDONG AGRICULTURAL UNIVERSITY
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