Medicine composition for preventing or treating pyohemia

A composition and sepsis technology, applied in the field of biomedicine, can solve the problems of systemic inflammatory response and circulatory metabolic state changes, inability to simulate clinical characteristics well, rapid death of animals, etc., and achieve the effect of inhibiting organ failure

Active Publication Date: 2018-09-07
FUJIAN NORMAL UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The toxemia and bacterial infection models can be artificially and precisely controlled the infection dose, with good stability and repeatability. However, from the perspective of the clinical onset of sepsis, there are infection lesions in the patient's body, and the infection lesions will continuously release bacteria and endotoxins , causing changes in the systemic inflammatory response and circulating metabolic state
Transient infusion of endotoxin or flora causes rapid animal death and does not mimic clinical features well

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicine composition for preventing or treating pyohemia
  • Medicine composition for preventing or treating pyohemia
  • Medicine composition for preventing or treating pyohemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The test method is as follows:

[0022] Effect of combination of huperzine A HupA and antibiotic TIENAM on the survival rate of CLP sepsis mice

[0023] 100 mice were randomly divided into 5 groups, sham operation control group (Sham group), normal saline control group (CLP group), HupA treatment group (0.1mg / kg·d), TIENAM group (50mg / kg·d) Combined with HupA+TIENAM group, 20 mice in each group, all mice were fasted for 12 hours before operation, and had free access to water. The blank control group received no treatment. The CLP sepsis mouse model was prepared according to the instructions in the literature. After laparotomy in the sham-operated control group, the cecum was freed, ligated, and no perforation was performed, and then the abdominal cavity was returned, and the abdomen was closed and sutured. At the end of the operation, 120 mice were subcutaneously injected with 1 ml of 37°C preheated normal saline on the back for fluid resuscitation; intraperitoneal in...

Embodiment 2

[0028] Detection of expression levels of inflammatory factors in serum

[0029] According to the operation steps of the ELISA kit manual, the expression levels of inflammatory factors IL-6, TNF-α, and IL-1β in peripheral blood serum were detected.

[0030] The test results are as follows:

[0031] Effects of HupA combined with TIENAM on peripheral blood cytokine levels in mice

[0032] As shown in Figure 2, 48 hours after operation, the levels of inflammatory factors such as TNF-α, IL-1β, and IL-6 in the serum of mice in the sham operation group were lower; were significantly increased, and the difference was significant ( P ), with statistical significance; TNF-ɑ, IL-1β, and IL-6 levels in the serum of mice in HupA group and HupA+TIENAM group were significantly lower than those in the CLP sepsis mouse model, and the difference was statistically significant ( P＜0.01 ).

Embodiment 3

[0034] Histopathological examination

[0035] Forty-eight hours after the operation, they were killed by dislocation of the neck, and the lungs and kidneys of the mice were collected, fixed with 4% paraformaldehyde immediately, and numbered, dehydrated, transparent, soaked in wax, embedded, sliced, and dewaxed on baked slices according to the conventional methods of pathological examination. HE staining, tissue lesions were observed under an optical microscope.

[0036] The test results are as follows:

[0037] Observation on the effect of HupA combined with TIENAM on the histopathology of CLP sepsis mice

[0038] Grouped according to the experiment, CLP sepsis mice were sacrificed by neck dislocation 48 hours after administration, and the main organs of each group, such as lung, liver, spleen, and kidney, were collected for HE staining, and their pathological changes were observed under a microscope.

[0039] (1) if image 3 As shown, the mice were randomly divided into 6 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to a medicine composition for preventing or treating pyohemia. The invention discloses application of Huperzine A (HupA) and (TIENAM) for preparing medicine for preventing or treating pyohemia through combined use, and medicine prepared by the method. The HupA and the TIENAM have the obvious treatment effect when being used in a combined way, so that the Cecal Ligation and Puncture (CLP) mouse survival rate is further improved to 50 percent (P is smaller than 0.05) from 15 percent; the expression of inflammatory factors in blood serum is inhibited to different degrees; the pathologic damage of the pyohemia on tissues of lungs, livers and the like can be obviously reduced; the organ failure is inhibited.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a pharmaceutical composition for preventing or treating sepsis. Background technique [0002] The pathogenesis of sepsis is very complex, clinically manifested as inflammation-mediated tissue damage, organ failure and immunosuppressive state, which is characterized by uncontrolled production of cytokines, causing tissue damage, and migration with intestinal bacteria / toxins. It is related to pathological processes such as network regulation of inflammatory response, immune dysfunction, coagulation dysfunction, multi-organ and system function changes and their mutual influence, and gene polymorphism. The pathogenesis has not yet been clarified. In February 2016, the American Society of Intensive Care Medicine (SCCM) and the European Society of Intensive Care Medicine (ESICM) jointly released the latest definition and diagnostic criteria for sepsis, arguing that seps...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4748A61K31/407A61K31/198A61P29/00A61P31/00
CPCA61P29/00A61P31/00A61K31/198A61K31/407A61K31/4748A61K2300/00
Inventor 陈骐苏经迁郭凯黄敏刘辉英
Owner FUJIAN NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap