Calcium phosphate-based composite microsphere support and preparation method thereof

A technology of composite microspheres and calcium phosphate, applied in the field of preparation of biomedical materials, can solve the problems of low pore size, limited collapse resistance, limited mechanical strength, etc., and achieve moderate pore size, high collapse resistance and enhanced mechanical properties. Effect

Active Publication Date: 2013-11-20
广州智焜生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the stents made by the above-mentioned prior art generally have the probl

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  • Calcium phosphate-based composite microsphere support and preparation method thereof

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Example Embodiment

[0037] Example 1

[0038] Dissolve 0.3g polyglycolide-lactide in 10mL dichloromethane to obtain a polyglycolide-lactide solution; combine 0.3g partially crystalline calcium phosphate (PCCP) and 0.2g anhydrous calcium hydrogen phosphate (DCPA) Disperse in polyglycolide-lactide solution to obtain solution 1; add 8mL Tween-80 to 400mL liquid paraffin to obtain solution 2; add solution 1 to solution 2 under stirring and continue stirring at 400rpm The composite microspheres were obtained in 10 hours, washed 4 times with anhydrous ether, and then freeze-dried; the 0.28g microspheres of 350~400um obtained after sieving were poured into the mold, 1mL deionized water was added, and the cylinder was placed for 12 minutes. Press 500kPa for 4s; after demolding, it was cured in a constant temperature and humidity box at 37℃ and 97% humidity for 18h, soaked in cold acetone at -75℃ for 4h and freeze-dried for 50h to obtain calcium phosphate-based microsphere scaffold.

[0039] Stent degradation...

Example Embodiment

[0040] Example 2

[0041] Dissolve 0.4g polyglycolide-lactide in 10mL of dichloromethane to obtain a polyglycolide-lactide solution; combine 0.35g partially crystalline calcium phosphate (PCCP) and 0.25g anhydrous calcium hydrogen phosphate (DCPA) Disperse in polyglycolide-lactide solution to obtain solution 1; add 6mL Tween-80 to 300mL liquid paraffin to obtain solution 2; add solution 1 to solution 2 under stirring, continue stirring at 300rpm The composite microspheres were obtained in 8 hours, washed 3 times with anhydrous ether, and then freeze-dried; the 0.3g microspheres of 300~350um obtained after sieving were poured into the mold, and 1mL deionized water was added. After standing for 15 minutes, the cylinder was Apply pressure at 800kPa for 5s; after demolding, cure in a constant temperature and humidity cabinet at 37°C and 97% humidity for 12 hours, soak in cold acetone at -85°C for 5 hours and freeze-dry for 40 hours to obtain calcium phosphate-based microsphere scaffo...

Example Embodiment

[0043] Example 3

[0044] Dissolve 0.5g polyglycolide-lactide in 10mL dichloromethane to obtain a polyglycolide-lactide solution; mix 0.275g partially crystalline calcium phosphate (PCCP) and 0.275g anhydrous calcium hydrogen phosphate (DCPA) Disperse in polyglycolide-lactide solution to get solution 1; add 30mL Tween-80 to 500mL liquid paraffin to get solution 2; add solution 1 to solution 2 under stirring, continue stirring at 500rpm The composite microspheres were obtained in 8 hours, washed with anhydrous ether 5 times and then freeze-dried; the 400-450um 0.35g microspheres obtained after sieving were poured into the mold, 1mL deionized water was added, and the cylinder was placed for 10 minutes. 700kPa pressure for 6s; after demolding, it was cured in a constant temperature and humidity cabinet at 37°C and 97% humidity for 24 hours, soaked in cold acetone at -80°C for 6 hours and then freeze-dried for 60 hours to obtain calcium phosphate-based microsphere scaffolds.

[0045] ...

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Abstract

The invention discloses a calcium phosphate-based composite microsphere support and a preparation method thereof. The preparation method comprises the following steps of dissolving polyglycollide-lactide in dichloromethane to obtain a polyglycollide-lactide solution; dispersing crystallized calcium phosphate and anhydrous calcium hydrogen phosphate in the polyglycollide-lactide solution to obtain a solution 1; adding Tween-80 to liquid wax to obtain a solution 2: adding the solution 1 to the solution 2 under a stirring state to obtain composite microspheres; pouring the composite microspheres into a mold, adding deionized water, conserving in a constant temperature and humidity chamber after demolding; immersing in cold acetone and freeze drying to obtain the calcium phosphate-based composite microsphere support. The prepared microsphere support has a three-dimensional through pore structure with moderate pore size, and has good mechanical properties and good biocompatibility. The pore size of the support is further enlarged after polyglycollide-lactide is degraded, thereby facilitating the formation of new bones. Besides, the preparation process is simple; raw materials are easily available; cost is low and industrialized production can be easily realized.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and in particular relates to a calcium phosphate-based composite microsphere scaffold and a preparation method thereof. Background technique [0002] Bone is one of the most important tissues in the human body. It plays an important role in protecting internal organs, providing attachment for muscles, and producing blood cells. There are countless bone defects or incomplete limbs caused by trauma, aging, exercise, inflammation, tumors and congenital deformities in the human body. Therefore, there is a huge demand for bone repair materials. Bone repair materials can replace or repair human diseased bone tissue and achieve regenerative functions. The research and development of bone repair materials is a global issue, and it is also a very active field in biomaterial research. According to the report of the civil affairs department, there are about 15 million patients w...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61L27/18A61L27/56
Inventor 魏坤许为康
Owner 广州智焜生物科技有限公司
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