N,O-carboxymethyl chitosan-polyaldehyde hyaluronic acid gel and use thereof

A technology of carboxymethylated chitosan and carboxymethyl chitosan, applied in the field of N,O-carboxymethylated chitosan-polyaldehyde-based hyaluronic acid gel, can solve the influence of film adhesion , It is impossible to completely cover the wound surface, and the residence time is short, so as to achieve the effect of good structural integrity, good cell compatibility, and anti-adhesion effect.

Active Publication Date: 2014-07-02
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

For polymer solutions, such as sodium hyaluronate as the raw material, the polymer named "Medical Sodium Hyaluronate Gel" (its trade name is Spek, Spect, Haailu, Kuaikeling, etc.) , due to its rapid degradation and absorption in the body, the residence time in the body is short, resulting in poor effect
For a solid film, it is impossible to completely cover the wound, and for bleeding wounds, the adhesion of the film is affected, and because the peristaltic film may fall off from the wound, sometimes the film needs to be sutured on the wound, which increases the difficulty of operation
Moreover, in laparoscopic surgery, the application of the membrane is extremely inconvenient
In addition, the residue of solid film (more than 30 days) may be potentially dangerous to wound healing and human body
Therefore, an ideal anti-adhesion barrier has not been found so far.

Method used

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  • N,O-carboxymethyl chitosan-polyaldehyde hyaluronic acid gel and use thereof
  • N,O-carboxymethyl chitosan-polyaldehyde hyaluronic acid gel and use thereof
  • N,O-carboxymethyl chitosan-polyaldehyde hyaluronic acid gel and use thereof

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preparation example Construction

[0059] The preparation method of N,O-carboxymethylated chitosan-polyaldehyde hyaluronic acid gel, the specific operation steps are as follows:

[0060] a. React chloroacetic acid and chitosan with sodium hydroxide aqueous solution at room temperature for 1-4 hours, wherein the mass ratio of chitosan to chloroacetic acid is 0.1-10:1; then increase the reaction temperature to 50-80°C to continue the reaction Terminate the reaction after 1-4 hours; filter out the solid, wash the solid with 60-90% ethanol or methanol aqueous solution; dissolve the obtained solid in water, use a dialysis bag with a molecular weight cut-off of 8000-14000 to perform dialysis treatment in water for 3 days, and then freeze-dry , to obtain N, O-carboxymethyl chitosan. Wherein, the aqueous sodium hydroxide solution of chitosan is obtained by adding chitosan to 20-60% aqueous sodium hydroxide solution and placing it at -20°C-0°C for 1-24h; the chitosan The mass ratio to sodium hydroxide is 0.1-10:1.

[...

Embodiment 1

[0083] a. Preparation of N,O-carboxymethylated chitosan: Put 5g of chitosan (molecular weight 1 million, x≈2130) in a 250mL round bottom flask, add 25mL of 40% sodium hydroxide aqueous solution, and put the flask Store in a refrigerator at -20°C for overnight treatment. In addition, dissolve 20g of chloroacetic acid with a certain amount of water and place it in a constant pressure funnel. Slowly add the aqueous solution of chloroacetic acid dropwise under stirring. After the dropwise addition, react under stirring conditions at room temperature 2h, and then increase the reaction temperature to 60°C to continue the reaction for 2h and terminate the reaction. The mixed system in the flask was suction-filtered, and the obtained filter residue was repeatedly washed 3 times with 80% ethanol aqueous solution. After washing, dissolve the obtained primary product with water, perform dialysis treatment in water for 3 days with a dialysis bag with a molecular weight cut-off of 8000-140...

Embodiment 2

[0087] a. Preparation of N,O-carboxymethylated chitosan: Put 5g of chitosan (molecular weight 1 million, x≈2130) in a 250mL round bottom flask, add 10mL of 20% sodium hydroxide aqueous solution, and put the flask Store in a -20°C refrigerator for overnight treatment. In addition, dissolve 5g of chloroacetic acid with a certain amount of water and place it in a constant pressure funnel. Slowly add the aqueous solution of chloroacetic acid dropwise under magnetic stirring conditions. After the dropwise addition, stir at room temperature. The reaction was carried out for 1 h, and then the reaction temperature was increased to 50 °C for 1 h, and then the reaction was terminated. The filter residue obtained from the suction filtration treatment of the mixed system in the flask was repeatedly washed 3 times with 60% ethanol aqueous solution. After washing, dissolve the obtained primary product with water, perform dialysis treatment in water for 3 days with a dialysis bag with a mole...

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Abstract

The invention belongs to the field of medical materials, particularly relates to N,O-carboxymethyl chitosan-polyaldehyde hyaluronic acid gel and use thereof, and aims at providing an anti-adhesion blocking agent which is good in anti-adhesion effect, good in biocompatibility and bioabsorbable. The N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel provided by the invention has structural unit shown in a formula I described in the specification, wherein x=10-10,000 and y=10-10,000. The invention also provides a preparation method of the N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel, and use thereof in prevention and treatment of postoperative tissue adhesion. The prepared N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel provides a novel choice for the anti-adhesion blocking agent.

Description

technical field [0001] The invention belongs to the field of medical materials, and specifically relates to N, O-carboxymethylated chitosan-polyaldehyde hyaluronic acid gel and its application. Background technique [0002] Abdominal adhesion is a common complication of common abdominal and pelvic surgery, the incidence rate is about 67-93%, and it is as high as 97% in pelvic surgery. Abdominal adhesions can occur not only between adjacent surgical trauma tissues, but also in tissues far away from the surgical area. Although most abdominal adhesions are asymptomatic, adhesions can lead to infertility, chronic pelvic and abdominal pain, and intestinal obstruction. The formation of postoperative adhesions increases the difficulty of reoperation and increases the medical expenses of patients. [0003] Abdominal adhesions generally occur in the early postoperative period. Therefore, intraoperative application of anti-adhesion drugs or devices has become the first choice for th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08G81/00C08B37/08A61P41/00A61L31/04A61L31/16A61L31/14A61K31/728A61K9/06
Inventor 巩长旸丁健刘新宇吴秦洁魏于全李玲
Owner SICHUAN UNIV
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