Cordycepin/O-carboxymethyl chitosan nanoparticle and preparation method thereof

A technology of carboxymethyl chitosan and cordycepin, which is applied in the directions of sugar derivatives, pharmaceutical formulations, and inactive medical preparations, can solve the problems of unfavorable environmental protection and safety, complicated preparation methods, easy to catch fire and explode, etc. To achieve the effect of small injury, safe reaction conditions and high drug loading

Active Publication Date: 2017-10-24
SHIJIAZHUANG ZANGNUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the corresponding preparation method of the cordycepin carboxymethyl chitosan nanoparticles is relatively complicated, and the production cost is relatively high. In the preparation process, an organic solvent is used, which is easy to catch fire and explode, has a high risk, and is not conducive to environmental protection and environmental protection. Safety

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The present embodiment provides a kind of preparation method of cordycepin / O-carboxymethyl chitosan nanoparticle, described method comprises the steps:

[0035] 1) 4g of O-carboxymethyl chitosan was dissolved in 1000mL of 0.9% sodium chloride solution to obtain a colorless, odorless and clear gel-like solution;

[0036] 2) Add 1 g of cordycepin to the gel-like solution obtained in step 1) at 30°C, and stir at a speed of 600 r / min for 20 min to make it evenly stirred;

[0037] 3) Add 300mL of sodium tripolyphosphate aqueous solution with a concentration of 2.5mg / mL into the system obtained in step 2), and stir for 30min to obtain a stable emulsified system;

[0038] 4) Centrifuge the emulsified system obtained in step 3), wash three times with deionized water, and dry in vacuum to obtain the cordycepin / O-carboxymethyl chitosan nanoparticles.

[0039] The quality of the described cordycepin / O-carboxymethyl chitosan nanoparticles that the present embodiment makes is 5.3g,...

Embodiment 2

[0041] The present embodiment provides a kind of preparation method of cordycepin / O-carboxymethyl chitosan nanoparticle, described method comprises the steps:

[0042] 1) 5g of O-carboxymethyl chitosan was dissolved in 1000mL of 0.9% sodium chloride solution to obtain a colorless, odorless and clear gel-like solution;

[0043] 2) Add 1 g of cordycepin to the gel-like solution obtained in step 1) at 30°C, and stir at a speed of 600 r / min for 20 min to make it evenly stirred;

[0044] 3) Add 400 mL of sodium tripolyphosphate aqueous solution with a concentration of 2.5 mg / mL to the system obtained in step 2), and stir for 30 minutes to obtain a stable emulsified system;

[0045] 4) Centrifuge the emulsified system obtained in step 3), wash three times with deionized water, and dry in vacuum to obtain the cordycepin / O-carboxymethyl chitosan nanoparticles.

[0046] The mass of the cordycepin / O-carboxymethyl chitosan nanoparticles prepared in this embodiment is 6.7g, the particle ...

Embodiment 3

[0048] The present embodiment provides a kind of preparation method of cordycepin / O-carboxymethyl chitosan nanoparticle, described method comprises the steps:

[0049] 1) 6g of O-carboxymethyl chitosan was dissolved in 1000mL of 0.9% sodium chloride solution to obtain a colorless, odorless and clear gel-like solution;

[0050] 2) Add 1 g of cordycepin to the gel-like solution obtained in step 1) at 30°C, and stir at a speed of 600 r / min for 20 min to make it evenly stirred;

[0051] 3) Add 467mL of sodium tripolyphosphate aqueous solution with a concentration of 2.5mg / mL to the system obtained in step 2), and stir for 30min to obtain a stable emulsified system;

[0052] 4) Centrifuge the emulsified system obtained in step 3), wash three times with deionized water, and dry in vacuum to obtain the cordycepin / O-carboxymethyl chitosan nanoparticles.

[0053] The quality of the described cordycepin / O-carboxymethyl chitosan nanoparticles that the present embodiment makes is 7.6g, and...

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Abstract

The invention discloses a cordycepin / O-carboxymethyl chitosan nanoparticle and a preparation method thereof. The grain size of the cordycepin / O-carboxymethyl chitosan nanoparticle is 100-200nm. The preparation method for the cordycepin / O-carboxymethyl chitosan nanoparticle comprises the following steps: dispersing cordycepin into a sodium chloride solution containing O-carboxymethyl chitosan; adding a sodium tripolyphosphate solution into the system; centrifuging, washing and vacuum-drying, thereby acquiring the cordycepin / O-carboxymethyl chitosan nanoparticle. According to the invention, the cordycepin / O-carboxymethyl chitosan nanoparticle prepared in the manner of using the O-carboxymethyl chitosan as a cordycepin nano-drug carrier, using sodium tripolyphosphate as a cross-linking agent and adopting an ionic cross-linking method is characterized by small grain size, uniform distribution, higher drug loading capacity and excellent slow release performance. The method disclosed by the invention is under mild and safe reaction conditions, uses no acid-base or organic solvent and is high in operability.

Description

technical field [0001] The invention relates to the technical field of biomedical nano sustained release, in particular to a cordycepin / O-carboxymethyl chitosan nanoparticle and a preparation method thereof. Background technique [0002] Cordycepin, also known as cordycepin, 3′-deoxyadenosine, is a nucleoside antibiotic. It has been proved by research that cordycepin has anti-tumor, immune regulation, anti-leukemia, antibacterial, anti-virus, anti-inflammatory, hypoglycemic, hypolipidemic , anti-aging and other effects, has a good prospect for clinical application. However, under the metabolism of adenosine deaminase in the body, cordycepin is easily deaminated rapidly and becomes the inactive metabolite 3′-deoxyinosine nucleoside, only a small part is converted into 3-phosphate cordycepin, and the efficacy of the drug can be exerted. It needs to be maintained at a certain concentration, which limits its clinical use alone, and it is prone to serious adverse reactions when ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K31/7076A61K47/36A61P35/00A61P37/02A61P35/02A61P31/04A61P31/10A61P31/12A61P29/00A61P3/10A61P3/06A61P39/06
CPCA61K9/0002A61K9/146A61K31/7076A61K31/00A61K47/36C07H19/16
Inventor 王智森高飞
Owner SHIJIAZHUANG ZANGNUO BIOTECH
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