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Endotoxin absorbing agent for blood perfusion and its preparation method

A hemoperfusion and adsorbent technology, applied in the field of biomedical materials, can solve the problems of unsuitable promotion, ineffective effect, high cost, etc., and achieve the effects of good blood compatibility, reduced treatment cost, and high scavenging capacity

Inactive Publication Date: 2004-05-05
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above two methods require plasma perfusion after separation of blood cells, which is very expensive and not suitable for domestic promotion.
There is only domestic animal experiment report on the adsorption of endotoxin by activated carbon whole blood perfusion, which shows that activated carbon perfusion can directly adsorb endotoxin in blood, but the effect is not obvious [Wang Jinda. Chinese Critical Care Medicine, 1998, 10: 578]
Endotoxin adsorbent for hemoperfusion with spherical agar as carrier has not been reported

Method used

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  • Endotoxin absorbing agent for blood perfusion and its preparation method
  • Endotoxin absorbing agent for blood perfusion and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1-1 Preparation of spherical agar gel carrier

[0032] Place a 500ml three-necked flask in a 60°C water bath, add 100ml of toluene, 50ml of carbon tetrachloride, and 0.5ml of Tween 80 into the bottle, and stir evenly.

[0033] Weigh 4 grams of agar powder and add 30ml of distilled water, heat and melt, pour the melted agar into the toluene-carbon tetrachloride system, stir, and disperse the agar solution into droplets of suitable size in the organic phase. Cool to room temperature, pour out the organic solvent in the upper layer, screen the agar balls of 0.45-1.0mm, wash with water, transfer to the Erlenmeyer flask, and store in a wet state at 4°C.

[0034] 1-2 Epichlorohydrin activation

[0035] Take 20.0 grams of agar balls, add 29.6 milliliters of 2M sodium hydroxide, then add 13.3 milliliters of epichlorohydrin, react at 40 ° C for 2 hours, wash with water until neutral, and thoroughly wash off the remaining epichlorohydrin, Epoxy-activated gel balls were obtained...

Embodiment 2

[0058] Effect of glutaraldehyde cross-linking on the properties of agar ball carrier

[0059] 2-1 Take 50 mg of agar balls from 1-1 before glutaraldehyde cross-linking and 1-4 after glutaraldehyde cross-linking respectively, wash them, dry them in vacuum and grind them into pieces. The TG209 thermogravimetric analyzer was used to measure the thermogravimetric curve of the carrier ball, the heating rate was 14.0°C / min, and the temperature rising range was 20-620°C. After crosslinking, the peak value of thermal weight loss increased from 277.4°C to 295.8°C, which indicated that the thermal stability of the agar balls was improved after crosslinking (see figure 1 ,2). figure 1 . Thermogravimetric curves of agar ball carrier before and after crosslinking. figure 2 .Derivative thermogravimetric curve of agar ball carrier before and after crosslinking.

[0060] Thermogravimetric method is one of the most important methods to evaluate the thermal stability of polymer materials. ...

Embodiment 3

[0063] blood compatibility test

[0064] Take 2 ml of the adsorbent prepared in Example 1, soak it in physiological saline for 12 hours, put it into the perfusion column, inject 5 ml of rabbit whole blood mixed with anticoagulant with a syringe, and perfuse it at a flow rate of 15 ml / min for 2 hours. At the same time, an empty perfusion column was used as a control column. The changes of blood components before and after perfusion were measured by MEK-6318K hematology analyzer. The results showed that before and after perfusion, various blood components did not change much, and the decreasing percentages were all within 5%, which indicated that this series of adsorbents had good blood compatibility and could be used for whole blood perfusion.

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Abstract

An endotoxin adsorbent used for blood perfusion is prepared from agat through adding the aqueous solution to the liquid mixture of toluene and CCl4 to obtain spherical agar, activating by epoxy chloropropane under alkaline condition, reacting on diamine and then on glutaraldehyde to obtain high-strength agar beads used as the carrier of adsorbent, reacting on the amino contained ligand (polymyxin B), and reducing by NaBH4. Its advantages are high compatibility to blood and high effect on absorbing endotoxin from blood.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to an endotoxin adsorbent for hemoperfusion and a preparation method thereof. Background technique [0002] Endotoxemia is one of the most common clinical fatal diseases, with a mortality rate of 40%-90%. In the United States, more than 100,000 people die from this disease every year [Skelter et al, Arch.Microbiol.1995, 164, 383-389.], there is no effective treatment in clinical practice. Endotoxemia is caused by the release of endotoxins from the outer cell walls of Gram-negative bacteria into the blood. After endotoxin enters the blood, it causes a series of reactions that eventually lead to organ necrosis, irreversible shock and death. How to timely and effectively remove or destroy the endotoxin in the patient's body is a key issue in the treatment of endotoxemia. In recent years, a variety of molecular biological agents to antagonize endotoxin have been developed abroad, ...

Claims

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Application Information

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IPC IPC(8): A61M1/34
Inventor 袁直刘涛侯光辉王孝杰张文升
Owner NANKAI UNIV
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