Macroreticular polyvinyl alcohol bead carrier and preparation thereof

Abstract

The invention provides a method for preparing a macroporous reticulated polyvinyl alcohol spherical vector. The method comprises the following steps: firstly, polyvinyl alcohol, calcium carbonate, sodium alginate and water are mixed in a certain mass proportion, and stirring is performed for full dissolution of the polyvinyl alcohol and uniform mixture, and then white PVA sol is obtained; secondly, the sol obtained is added into saturated boric acid solution containing 3 percent of calcium chloride by a peristaltic pump, and PVA gel beads are formed, cleaned by water and then placed into diluted hydrochloric acid solution for dipping until no air bubble is generated; thirdly, a spherical vector is thrown into glutaral pentanedial water solution and adjusted into acidity so as to generate crosslinking reaction to form a more stable crosslinking structure; and fourthly, the spherical vector is dipped into water and cleaned into neutrality, and then the white macroporous reticulated PVA spherical vector with elasticity is obtained. The macroporous reticulated polyvinyl alcohol vector prepared by the method has stable macroporous reticulated structure and good hydrophilicity, physical and chemical stability and anti-biological degradability, is suitable for immobilized enzymes and microorganisms so as to form a plurality of bed-shaped bioreactors, and is used in the modern bioengineering field such as sewage treatment and so on.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to the preparation of a biological carrier, in particular to a macroporous net-like polyvinyl alcohol spherical carrier and its preparation; it is mainly used for immobilizing enzymes and microorganisms to form various bed-type bioreactors. Background technique [0002] Polyvinyl alcohol (PVA) carrier has the characteristics of being soluble in water, non-toxic to organisms, cheap, and easy to industrialize, so it is widely used in the immobilization of biologically active substances. In recent years, a lot of research has been done on the immobilization of biologically active substances on PVA carriers at home and abroad. The preparation methods mainly include PVA-boric acid method and freezing and thawing method. The boric acid method of embedding bioactive substances has high mechanical strength, long service life and good elasticity. The disadvantage is that boric acid has ...

AI Technical Summary

Problems solved by technology

Although these methods all show advantages in one aspect, they often neglect the other and fail to solve the toxicity and instability of the carrier at the same time.

In addition, as an immobilized carrier, in addition to its non-toxicity and stability, its mass transfer and loadability are very important, but the current embedding method in the study of immobilized microorganisms is bound to soak the immobilized microorganisms in chemical reagents Or freeze to affect the activity and application efficiency of microorganisms, and the porosity of the embedding carrier is small, the mass transfer and load capacity are limited

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