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39results about How to "Improve biological reaction efficiency" patented technology

Macroreticular polyvinyl alcohol bead carrier and preparation thereof

The invention provides a method for preparing a macroporous reticulated polyvinyl alcohol spherical vector. The method comprises the following steps: firstly, polyvinyl alcohol, calcium carbonate, sodium alginate and water are mixed in a certain mass proportion, and stirring is performed for full dissolution of the polyvinyl alcohol and uniform mixture, and then white PVA sol is obtained; secondly, the sol obtained is added into saturated boric acid solution containing 3 percent of calcium chloride by a peristaltic pump, and PVA gel beads are formed, cleaned by water and then placed into diluted hydrochloric acid solution for dipping until no air bubble is generated; thirdly, a spherical vector is thrown into glutaral pentanedial water solution and adjusted into acidity so as to generate crosslinking reaction to form a more stable crosslinking structure; and fourthly, the spherical vector is dipped into water and cleaned into neutrality, and then the white macroporous reticulated PVA spherical vector with elasticity is obtained. The macroporous reticulated polyvinyl alcohol vector prepared by the method has stable macroporous reticulated structure and good hydrophilicity, physical and chemical stability and anti-biological degradability, is suitable for immobilized enzymes and microorganisms so as to form a plurality of bed-shaped bioreactors, and is used in the modern bioengineering field such as sewage treatment and so on.
Owner:LANZHOU UNIVERSITY

Wastewater denitrification nitrogen removal treatment device and method

The invention discloses a wastewater denitrification nitrogen removal treatment device and method. The wastewater denitrification nitrogen removal treatment device mainly comprises an oxygen-poor expansion bed, a feeding pump, a circulating pump, a feeding tank, an air floatation tank, a heating pump, a heater and a control data cabinet. The oxygen-poor expansion bed is operated at an expansion state through regulating and controlling the flow of circulating water, cutting-off of the water is avoided, and removing loads of organic matters and nitrate are improved. The oxygen-poor expansion bed has the advantages of large height-diameter ratio, small occupied area, high sewage treatment efficiency, high impact resistance, short retention time, simplicity and convenience for operation, high economic benefits and wide application prospects.
Owner:CHINA PETROLEUM & CHEM CORP +1

Nanometer magnetic fluorescent microsphere and its prepn and application

The present invention relates to nanometer magnetic fluorescent microsphere and its preparation process and application. The nanometer magnetic fluorescent microsphere is one core-shell including core of Eu3+Y-Fe2O3 compound, shell of SiO2 coated fluorescent material and surface modifying organic functional group. It is prepared through reverse micro emulsion process to synthesize nanometer particle, and the subsequent surface chemical modification to become magnetic nanometer particle capable of combining with probe. The nanometer magnetic fluorescent microsphere is used as biological research tool with integrated fluorescent indication and magnetic separation, and may have important application in biomicromolecule detection, medical diagnosis and other fields.
Owner:SHANGHAI NORMAL UNIVERSITY

A nitrification-denitrification two-phase expanded-bed reactor and a sewage treating process

A nitrification-denitrification two-phase expanded-bed reactor and a sewage treating process are provided. The expanded-bed reactor comprises a reactor, an oxygen dissolving machine, a circulating pump and auxiliary pipelines. The reactor has a vertical cylindric structure, and comprises a bottom water distribution zone, an aerobic reaction zone, a secondary water distribution zone, an anoxic reaction zone and a water discharging zone from bottom to top. The aerobic reaction zone and the anoxic reaction zone are filled with small-particle-size fillers. The secondary water distribution zone is provided with a shrinkage-expansion type collector ring. The water discharging zone is provided with a three-phase separator. Through adoption of the expanded-bed reactor, nitrification and denitrification are performed in the reactor, biochemical and oxygen dissolving processes are performed independently, a biochemical treating load is high, the floor space of equipment is small and investment and a running cost are saved.
Owner:SINOPEC ENG +1

Fluorescent nano-particle with surface biological function, its production and use

This invention relates to a preparation method and its application of nuclear shell fluorescence nanometer particle whose surface living things is functional. The particle has nuclear shell structure, fluorescence material is hit in the inner core; the outer shell is composed of fluorescence transparency material; and there is embellishing organic function group in the outer covering. The nanometer particle is formed by the reversal tiny emulsion method, and doing chemical modification in the surface to make it to be biology functional nanometer particle. The nanometer particle has important application prospect in the fields such as cell biology, ultramicrochemistry, within the AMD chemistry, living things big molecule detecting and medical in-vivo diagnosing.
Owner:SHANGHAI NORMAL UNIVERSITY

A waste water treatment system and a method

A waste water treatment device and a method are disclosed. The waste water treatment device comprises an ozone oxidation device and a biochemical treatment device. The ozone oxidation device comprises an ozone destructor and an oxidation reactor. The biochemical treatment device comprises a two-phase expanded bed, an oxygen dissolving device and a circulating pump. An exhaust outlet at the top of the oxidation reactor is connected to a gas inlet of the ozone destructor. A gas outlet of the ozone destructor is connected to a gas inlet at the lower part of the oxygen dissolving device. A water discharging pipeline at the bottom of the oxygen dissolving device and a water discharging pipeline at the lower part of the oxidation reactor are combined and then connected to the circulating pump. The outlet of the circulating pump is connected to a water inlet at the lower part of the two-phase expanded bed. A water outlet at the upper part of the two-phase expanded bed is connected to a water inlet at the upper part of the oxygen dissolving device. The method includes: feeding waste water into the oxidation reactor firstly to subject the waste water to oxidation, and feeding the waste water into the biochemical treatment device to subject the waste water to aerobic biochemical reactions. According to the waste water treatment device and the method, reaction conditions and mass transfer conditions can be optimized, the biochemical treatment load is increased, the oxygen component in the oxidation exhaust and the excess pressure of the exhaust are fully utilized, and the treatment cost is reduced.
Owner:SINOPEC ENG +1

Reciprocating biological contactor and method of use

A bioreactor vessel has a biological support provided within the vessel to support bacteria and a drive system for agitating the biological support while it is it is immersed in the reaction liquor that is to be treated within the vessel. The drive system causes the biological support to oscillate with a reciprocating or rectilinear motion and the drive system is preferably located either outside the vessel or within a portion of the vessel that is above the level of reaction liquor present therein.
Owner:BLUMETRIC ENVIRONMENTAL

Biological functional fluorescence magnetic particle and its preparing method and application

This invention relates to a biological functional fluorescent magnetic nanometer particle and preparation method and application. The particle has core-shell structure, including: fluorescent material and magnetic material of kernel layer; the shell layer formed by fluorescent transparent material; and the modified layer above the shell layer surface with organic functional group. Through reverse microemulsion, it cans synthesis nanometer particles with both fluorescent and magnetic function, and process chemical modification on its surface to make biological functional magnetic nanometer particles. The nanometer particles can be used for biological marking, biological imaging and biological separation, etc.
Owner:SHANGHAI NORMAL UNIVERSITY

Anaerobic ammonium oxidation biochemical treatment method for coking waste water

The invention discloses an anaerobic ammonium oxidation biochemical treatment method for coking waste water, and belongs to the field of coking waste water treatment. A water inlet channel, a hypoxiapreconditioning zone, an MBBR front aerobic zone, a prepositive anoxic zone, an MBBR nitrosation aerobic zone, an MBBR anaerobic ammonium oxidation zone, an MBBR rear aerobic zone, a sludge postpositive anoxic zone, a postpositive sludge aerobic zone and an advective condensation zone are involved; the prepositive anoxic zone is communicated with the MBBR nitrosation aerobic zone through a squarewater passing hole formed in the upper side of one side end of a separation wall arranged between the prepositive anoxic zone and the MBBR nitrosation aerobic zone, and a stirring device is arranged in the prepositive anoxic zone; the MBBR nitrosation aerobic zone is communicated with the MBBR anaerobic ammonium oxidation zone through a square water passing hole formed in the upper side of one side end of a separation wall arranged between the MBBR nitrosation aerobic zone and the MBBR anaerobic ammonium oxidation zone; the MBBR nitrosation aerobic zone is communicated with the MBBR rear aerobic zone through a square water passing hole formed in the upper side of one side end of a separation wall arranged between the MBBR nitrosation aerobic zone and the MBBR rear aerobic zone. According to the method, prepositive anaerobic ammonium oxidation and postpositive denitrification nitrogen removal are combined to ensure that the total nitrogen mount of the water discharged to an external environment after coking waste water treatment is conducted reaches standards.
Owner:济宁市孚源环保科技有限公司

Anoxic denitrification fluidized bed treatment apparatus

The invention relates to an anoxic denitrification fluidized bed treatment apparatus, which comprises a reactor main body (4), an ultrasonic wave oscillator (containing a vibration plate (19)) and a nitrogen generation system (5), wherein the water inlet (20) of a circulation pump (3) is positioned in a filtration screen (17) near a tower head, the pore size of the filtration screen (17) is slightly smaller than the diameter of a biological filler so as to effectively prevent the biological filler in the reactor from entering a circulation system, the ultrasonic wave oscillator is arranged nearby, sludge originally attached to the filtration screen (17) is dispersed and peeled off through the direct action and the indirect action such as the cavitation effect, the direct inflow effect andthe acceleration effect of the ultrasonic wave oscillator on liquids and dirt so as to clean the filtration screen (17) to make the circulation system operate stably for a long time, and with a waterflow distributor (9) arranged on the bottom portion of the reactor main body (4), the problems of non-uniform influent distribution of the reactor main body and the like can be effectively solved, theshort circuit of water flow can be avoided, and the removal load of organic matters and nitrates can be improved.
Owner:CHINA PETROLEUM & CHEM CORP +1

Aerobic and anaerobic VBBR series coupling device and sewage treatment method

The invention relates to a series coupling type sewage treatment device, which comprises an anaerobic reactor and an aerobic reactor. The anaerobic reactor is a baffling-type inner circulation bioreactor, and a water inlet is formed in the anaerobic reactor; the aerobic reactor is a baffling-type inner circulation bioreactor, and a water outlet is formed in the aerobic reactor; a connecting pipe is connected between the anaerobic reactor and the aerobic reactor; and a flow returning pipe is connected between the anaerobic reactor and the aerobic reactor, and the flow of a fluid returning in the flow returning pipe is 1.5-3 times of the flow of inflowing water in the water inlet. The series coupling type sewage treatment device is similar to an existing sewage treatment technology adoptingan A / O or A2 / O method, a denitrification reaction is achieved through organic carbon sources in the inflowing water, but compared with a traditional A / O or A2 / O process, no sludge is contained in circulating liquid, it can be guaranteed that denitrification bacteria and nitration bacteria in the anaerobic reactor and the aerobic reactor are not disturbed, and a stable microbial flora is maintained. Through series coupling of the two reactors, the sewage treatment effect is much higher than the sewage treatment effect achieved by the traditional A / O or A2 / O process.
Owner:SHANGHAI NORMAL UNIVERSITY

Up-flow anaerobic biological reaction/precipitator used for wastewater treatment

The invention discloses an up-flow anaerobic biological reaction / precipitator used for wastewater treatment. The up-flow anaerobic biological reaction / precipitator is rectangular in shape; an integrated structure of the up-flow anaerobic biological reaction / precipitator comprises a reaction zone, a water distribution zone, a settling zone, a water inlet and backflow system, a water outlet system, and a sludge and gas discharge system; the reaction zone is arranged at the lower part of the up-flow anaerobic biological reaction / precipitator; the settling zone is arranged at the middle of the upper part of the up-flow anaerobic biological reaction / precipitator; the bottom of the settling zone is provided with a water distribution / sludge backflow system; the water distribution zone is arranged between the reaction zone and the settling zone; the water inlet and backflow system is arranged in the reaction zone; and the water outlet system is arranged at the upper part of the up-flow anaerobic biological reaction / precipitator and on the diagonal corners. Up-flow reaction conditions can be obtained using the up-flow anaerobic biological reaction / precipitator, and are beneficial for biological reaction process mass transferring; biological reaction efficiency is increased; after biological reaction, wastewater is subjected to precipitation in the integrated settling zone, sludge obtained via precipitation is delivered back to the reaction zone, and it is beneficial for maintenance of microbial biomass.
Owner:CHINA PETROCHEMICAL CORP +1

Method for improving expression quantity of pichia pastoris foreign protein by using mercaptan peroxidase

ActiveCN103898142AContributes to correct conformational formationPromote secretionVector-based foreign material introductionPichia pastorisBiotechnology
The invention discloses a method for improving the expression quantity of a pichia pastoris foreign recombinant protein by using mercaptan peroxidase as a cofactor aiming at improving the preparation capability of the pichia pastoris foreign recombinant protein, belonging to the field of biochemical engineering. The method comprises the steps of connecting a mercaptan peroxidase gene with an inducible promoter or a constitutive promoter from pichia pastoris or saccharomyces cerevisiae to construct a gene expression vector, and transferring the gene expression vector to a methanol induced pichia pastoris strain for expressing the foreign recombinant protein to perform co-expression or over-expression, wherein the mercaptan peroxidase gene serving as a global control gene of oxidation reduction stress response improves the expression quantity of the foreign recombinant protein in the methanol induced pichia pastoris under the control effects of the promoter of different strengths and the transcriptional level of a gene copy number. The invention provides a new method for improving the capability of producing recombinant protein by cells and the bio-reaction efficiency, which has an important value for strengthening a biotechnology process.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Anoxic fluidized bed device for treating high-concentration nitrate-nitrogen wastewater

The invention discloses an anoxic fluidized bed device for treating high-concentration nitrate-nitrogen wastewater. The anoxic fluidized bed device comprises a feeding tank, an anoxic fluidized bed, anitrogen generator system and a nitrogen outlet distributor, wherein the anoxic fluidized bed comprises a filling material area, a transition section and a water outlet area which are sequentially arranged from bottom to top; a three-phase separator is arranged in the water outlet area; a water inlet is arranged at the bottom end of the anoxic fluidized bed; a water flow distributor is arranged above the water inlet and below the filling material area; a water outlet is arranged at the upper part of the water outlet area; the feeding tank is connected with the water inlet of the anoxic fluidized bed through a feeding pipe; the nitrogen generator system is connected with the nitrogen outlet distributor through a gas inlet pipe; the nitrogen outlet distributor is located in the filling material area of the anoxic fluidized bed; and the position of the nitrogen outlet distributor in the anoxic fluidized bed is adjustable. The device provided by the invention realizes accurate demolding in a directional positioning way through arrangement of the position of the nitrogen outlet distributor according to needs.
Owner:CHINA PETROLEUM & CHEM CORP +1

Biological reaction device and biological reaction method

PendingCN112538413AImprove mass transfer rate and dissolved oxygen rateEmission reductionBioreactor/fermenter combinationsBiological substance pretreatmentsChemistryBiophysics
The invention provides a biological reaction device and a biological reaction method, the biological reaction device comprises a shell, a stirring module and a jet flow module located at the bottom ofthe shell are arranged in the shell, the stirring module comprises a stirring shaft extending into the shell, and at least one radial flow paddle and at least one axial flow paddle are arranged on the stirring shaft. The biological reaction method comprises the steps that reactants are injected into the shell, the stirring module is started to perform stirring reaction on the reactants, and gas generated by the reaction enters the jet flow module through a circulating pipeline and is jetted into the shell through the jet flow module. By combining the jet module and the stirring module, mass transfer and heat transfer are better achieved, the reactants are fully mixed and react, the gas circulates in the reaction device through the circulating pipeline, the production efficiency of the biological reaction device can be effectively improved, and the biological reaction device has the advantages of being low in cost, small in occupied area, easy to maintain and the like and can be applied to the fields of food, medicine and the like.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

Denitrification expanded bed and method for treating wastewater containing nitrate and nitrogen

The invention discloses a denitrification expanded bed and a method for treating waste water containing nitrate nitrogen. The denitrification expanded bed includes: an anoxic expanded bed reactor, and the anoxic expanded bed reactor is respectively bottom water distribution area, Anoxic reaction zone and three-phase separation zone; water outlet tank and self-cleaning filter, the inside of the water outlet tank is provided with a water outlet zone and a mixing zone, and the water outlet zone is connected to the three-phase separation zone and the mixing zone, so The mixing zone is communicated with the bottom water distribution zone through the self-cleaning filter; wherein, the bottom of the anoxic expanded bed reactor is provided with a water distribution plate for separating the bottom water distribution zone and the anoxic reaction zone; the anoxic A three-phase separator is installed at the top of the expanded bed reactor. The denitrification expanded bed can effectively avoid the phenomenon of water flow short circuit.
Owner:SINOPEC ENG +1

Point compensated linear aerating pipes

A point compensated linear aerating tube for sewage biological treating system consists of aerating notch, microporous aerating head and aerating offtakes. It is prepared by taking SiOx or ZnO nanometer composite material and polyolefin material, mixed extruding, linear aerating and point aerating coordinating reacting, and turbulent aerating agitating to promote deposited pollutant to take part in biological reaction. Its advantages include higher oxygen utilizing rate and biological reacting efficiency, aging and bacteria corroding resistant performances, and longer life.
Owner:LANZHOU UNIVERSITY

An anoxic fluidized bed device for high concentration nitrate nitrogen wastewater treatment

The invention discloses an anoxic fluidized bed device for treating high-concentration nitrate-nitrogen wastewater, which comprises: a feed tank, an anoxic fluidized bed, a nitrogen generator system and a nitrogen outlet distributor; an anoxic fluidized bed It includes a packing area, a transition section and a water outlet area arranged in sequence from bottom to top, and a three-phase separator is arranged in the water outlet area; a water inlet is provided at the bottom of the anoxic fluidized bed, and above the water inlet, A water flow distributor is provided under the filling area, and a water outlet is provided on the upper part of the water outlet area; the feed tank is connected to the water inlet of the anoxic fluidized bed through the feed pipe; the nitrogen generator system is connected to the nitrogen outlet distributor through the inlet pipe The nitrogen outlet distributor is located in the filling area of ​​the anoxic fluidized bed, and the position of the nitrogen outlet distributor in the anoxic fluidized bed can be adjusted. The device provided by the present invention sets the position of the nitrogen outlet distributor according to the needs, so as to realize the directional positioning and precise film removal.
Owner:CHINA PETROLEUM & CHEM CORP +1

Microbial inoculation device and method for treating slightly polluted water using the device

The invention discloses a microbiological inoculation device and a method for treating micro-polluted water by the microbiological inoculation device, and relates to the technical field of microbiological inoculation. The microbiological inoculation device comprises an ozone aeration tower, an industrial ozone generator, a porous ceramic catalyst unit, an ultraviolet catalyst unit, an inoculant tank, a culture solution tank, an inoculation biology filtration column, an air bottle and a biological filter chamber, an aeration tray is arranged at the inner bottom of the ozone aeration tower, the industrial ozone generator is communicated with the aeration tray in the ozone aeration tower, an outlet in the bottom of the ozone aeration tower is communicated with an inlet of the porous ceramic catalyst unit, an outlet of the porous ceramic catalyst unit is communicated with an inlet of the ultraviolet catalyst unit, and an outlet of the ultraviolet catalyst unit is communicated with a water pump I. The method includes the steps: firstly, ozone ultraviolet catalysis; second, bacterial inoculation and biodegradation treatment. The method solves the problems that nutrient substances in the micro-polluted water are insufficient, inoculated bacteria are easily affected by indigenous bacteria, and the method is used for treating the micro-polluted water.
Owner:HARBIN INST OF TECH

Anoxic expanded bed device and method for treating high-concentration nitrate wastewater

The invention discloses an anoxic expanded bed device and a method for treating wastewater. The problem of uneven water inlet distribution of an anoxic expanded bed is effectively solved, water flow short circuit is avoided, and organic matter and nitrate removal load is increased. A water and gas distribution distributor is arranged at the top of the anoxic expanded bed, so that biological fillers in the anoxic expanded bed are effectively prevented from being discharged out of a circulating system, and the circulating system stably runs for a long time. The anoxic fluidized bed is large in height-diameter ratio, small in occupied area, high in sewage treatment efficiency, high in impact resistance, short in residence time, easy and convenient to operate, high in economic benefit and widein application prospect.
Owner:CHINA PETROLEUM & CHEM CORP +1

Up-flow anaerobic biological reaction/precipitator used for wastewater treatment

The invention discloses an up-flow anaerobic biological reaction / precipitator used for wastewater treatment. The up-flow anaerobic biological reaction / precipitator is rectangular in shape; an integrated structure of the up-flow anaerobic biological reaction / precipitator comprises a reaction zone, a water distribution zone, a settling zone, a water inlet and backflow system, a water outlet system, and a sludge and gas discharge system; the reaction zone is arranged at the lower part of the up-flow anaerobic biological reaction / precipitator; the settling zone is arranged at the middle of the upper part of the up-flow anaerobic biological reaction / precipitator; the bottom of the settling zone is provided with a water distribution / sludge backflow system; the water distribution zone is arranged between the reaction zone and the settling zone; the water inlet and backflow system is arranged in the reaction zone; and the water outlet system is arranged at the upper part of the up-flow anaerobic biological reaction / precipitator and on the diagonal corners. Up-flow reaction conditions can be obtained using the up-flow anaerobic biological reaction / precipitator, and are beneficial for biological reaction process mass transferring; biological reaction efficiency is increased; after biological reaction, wastewater is subjected to precipitation in the integrated settling zone, sludge obtained via precipitation is delivered back to the reaction zone, and it is beneficial for maintenance of microbial biomass.
Owner:CHINA PETROCHEMICAL CORP +1

Macroreticular polyvinyl alcohol bead carrier and preparation thereof

The invention provides a method for preparing a macroporous reticulated polyvinyl alcohol spherical vector. The method comprises the following steps: firstly, polyvinyl alcohol, calcium carbonate, sodium alginate and water are mixed in a certain mass proportion, and stirring is performed for full dissolution of the polyvinyl alcohol and uniform mixture, and then white PVA sol is obtained; secondly, the sol obtained is added into saturated boric acid solution containing 3 percent of calcium chloride by a peristaltic pump, and PVA gel beads are formed, cleaned by water and then placed into diluted hydrochloric acid solution for dipping until no air bubble is generated; thirdly, a spherical vector is thrown into glutaral pentanedial water solution and adjusted into acidity so as to generate crosslinking reaction to form a more stable crosslinking structure; and fourthly, the spherical vector is dipped into water and cleaned into neutrality, and then the white macroporous reticulated PVA spherical vector with elasticity is obtained. The macroporous reticulated polyvinyl alcohol vector prepared by the method has stable macroporous reticulated structure and good hydrophilicity, physicaland chemical stability and anti-biological degradability, is suitable for immobilized enzymes and microorganisms so as to form a plurality of bed-shaped bioreactors, and is used in the modern bioengineering field such as sewage treatment and so on.
Owner:LANZHOU UNIVERSITY

Method for treating high-concentration nitrate wastewater

The invention discloses a method for treating high-concentration nitrate wastewater. The method includes the following steps: S1, adding a carbon source and nitrogen and phosphorus nutrients into thenitrate wastewater, and adjusting the pH of the wastewater to 2-4 to obtain pretreated wastewater; and S2, mixing the pretreated wastewater and circulating water, introducing the obtained mixed waterinto an anoxic expanded bed reactor to be in contact with a biological filler, and performing a denitrification reaction to obtain denitrified wastewater, wherein the circulating water is a product obtained by the denitrification reaction of the pretreated wastewater and the biological filler. The cost of treating the nitrate wastewater by using the method is lower, and the method can continuouslytreat the high-concentration nitrate (3000-35000 mg / L) wastewater, and has high treatment efficiency.
Owner:CHINA PETROLEUM & CHEM CORP +1

Denitrification treatment method of high-concentration nitrate wastewater

The invention discloses a denitrification treatment method of high-concentration nitrate wastewater. The denitrification treatment method of the high-concentration nitrate wastewater comprises the following steps: step S1, adjusting pH value of nitrate wastewater to 2-4, and adding carbon sources, as well as nitrogen and phosphorus nutrient salts, into the nitrate wastewater after pH-value adjustment so as to obtain wastewater after pretreatment; and step S2, mixing the wastewater after the pretreatment with circulating water so as to obtain mixed water, and introducing the mixed water into ananoxic fluidized bed reactor so as to allow contact between the mixed water and a biological filter, thereby causing denitrification reaction so as to obtain wastewater after denitrification treatment, wherein the circulating water is a product of the denitrification reaction between the wastewater after the pretreatment and the biological filter. Being used for treating high-concentration nitrate wastewater, the denitrification treatment method of the high-concentration nitrate wastewater is relatively low in cost. In addition, the denitrification treatment method of the high-concentration nitrate wastewater is capable of continuously treating high-concentration nitrate wastewater (of which the concentration is 3000-35000 mg / L); and moreover, the method is high in treatment efficiency.
Owner:CHINA PETROLEUM & CHEM CORP +1

A method for increasing the expression of exogenous protein in Pichia pastoris by thiol peroxidase

ActiveCN103898142BContributes to correct conformational formationPromote secretionVector-based foreign material introductionPeroxidasePichia
The invention discloses a method for improving the expression quantity of a pichia pastoris foreign recombinant protein by using mercaptan peroxidase as a cofactor aiming at improving the preparation capability of the pichia pastoris foreign recombinant protein, belonging to the field of biochemical engineering. The method comprises the steps of connecting a mercaptan peroxidase gene with an inducible promoter or a constitutive promoter from pichia pastoris or saccharomyces cerevisiae to construct a gene expression vector, and transferring the gene expression vector to a methanol induced pichia pastoris strain for expressing the foreign recombinant protein to perform co-expression or over-expression, wherein the mercaptan peroxidase gene serving as a global control gene of oxidation reduction stress response improves the expression quantity of the foreign recombinant protein in the methanol induced pichia pastoris under the control effects of the promoter of different strengths and the transcriptional level of a gene copy number. The invention provides a new method for improving the capability of producing recombinant protein by cells and the bio-reaction efficiency, which has an important value for strengthening a biotechnology process.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Disinfection treatment method for deep denitrification and dephosphorization of urban sewage

The invention discloses a disinfection treatment method for deep denitrification and phosphorus removal of urban sewage, which belongs to the field of sewage treatment. pool and denitrification pool, and there is an infusion pipe connected between two adjacent pools, the upper part of the ammonification pool is connected with a liquid inlet pipe, and the upper ends of the ammonification pool, nitrification pool and denitrification pool are all connected with feeding pipes, which can realize The residence time of oxygen bubbles in water can increase the dissolution rate of oxygen in water and the activity of biological bacteria, thereby improving the efficiency of biological reactions and the effect of sewage treatment. The attachment rods imitating the shape of cactus are used to provide attachment points for bubbles to increase The residence time of the bubbles in the water, and the attachment rod is set close to the position where the bubbles are generated to prevent the bubbles from floating up immediately. In addition, the opening and closing of the plastic cover is like a fish spitting out water bubbles, which can increase the volume of the bubbles and further improve the dissolution in water oxygen.
Owner:内蒙古丝绸之路建设工程有限公司
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