39results about How to "Improve biological reaction efficiency" patented technology

The invention provides a method for preparing a macroporous reticulated polyvinyl alcohol spherical vector. The method comprises the following steps: firstly, polyvinyl alcohol, calcium carbonate, sodium alginate and water are mixed in a certain mass proportion, and stirring is performed for full dissolution of the polyvinyl alcohol and uniform mixture, and then white PVA sol is obtained; secondly, the sol obtained is added into saturated boric acid solution containing 3 percent of calcium chloride by a peristaltic pump, and PVA gel beads are formed, cleaned by water and then placed into diluted hydrochloric acid solution for dipping until no air bubble is generated; thirdly, a spherical vector is thrown into glutaral pentanedial water solution and adjusted into acidity so as to generate crosslinking reaction to form a more stable crosslinking structure; and fourthly, the spherical vector is dipped into water and cleaned into neutrality, and then the white macroporous reticulated PVA spherical vector with elasticity is obtained. The macroporous reticulated polyvinyl alcohol vector prepared by the method has stable macroporous reticulated structure and good hydrophilicity, physical and chemical stability and anti-biological degradability, is suitable for immobilized enzymes and microorganisms so as to form a plurality of bed-shaped bioreactors, and is used in the modern bioengineering field such as sewage treatment and so on.

The invention discloses a wastewater denitrification nitrogen removal treatment device and method. The wastewater denitrification nitrogen removal treatment device mainly comprises an oxygen-poor expansion bed, a feeding pump, a circulating pump, a feeding tank, an air floatation tank, a heating pump, a heater and a control data cabinet. The oxygen-poor expansion bed is operated at an expansion state through regulating and controlling the flow of circulating water, cutting-off of the water is avoided, and removing loads of organic matters and nitrate are improved. The oxygen-poor expansion bed has the advantages of large height-diameter ratio, small occupied area, high sewage treatment efficiency, high impact resistance, short retention time, simplicity and convenience for operation, high economic benefits and wide application prospects.

The present invention relates to nanometer magnetic fluorescent microsphere and its preparation process and application. The nanometer magnetic fluorescent microsphere is one core-shell including core of Eu3+Y-Fe2O3 compound, shell of SiO2 coated fluorescent material and surface modifying organic functional group. It is prepared through reverse micro emulsion process to synthesize nanometer particle, and the subsequent surface chemical modification to become magnetic nanometer particle capable of combining with probe. The nanometer magnetic fluorescent microsphere is used as biological research tool with integrated fluorescent indication and magnetic separation, and may have important application in biomicromolecule detection, medical diagnosis and other fields.

A waste water treatment device and a method are disclosed. The waste water treatment device comprises an ozone oxidation device and a biochemical treatment device. The ozone oxidation device comprises an ozone destructor and an oxidation reactor. The biochemical treatment device comprises a two-phase expanded bed, an oxygen dissolving device and a circulating pump. An exhaust outlet at the top of the oxidation reactor is connected to a gas inlet of the ozone destructor. A gas outlet of the ozone destructor is connected to a gas inlet at the lower part of the oxygen dissolving device. A water discharging pipeline at the bottom of the oxygen dissolving device and a water discharging pipeline at the lower part of the oxidation reactor are combined and then connected to the circulating pump. The outlet of the circulating pump is connected to a water inlet at the lower part of the two-phase expanded bed. A water outlet at the upper part of the two-phase expanded bed is connected to a water inlet at the upper part of the oxygen dissolving device. The method includes: feeding waste water into the oxidation reactor firstly to subject the waste water to oxidation, and feeding the waste water into the biochemical treatment device to subject the waste water to aerobic biochemical reactions. According to the waste water treatment device and the method, reaction conditions and mass transfer conditions can be optimized, the biochemical treatment load is increased, the oxygen component in the oxidation exhaust and the excess pressure of the exhaust are fully utilized, and the treatment cost is reduced.

A bioreactor vessel has a biological support provided within the vessel to support bacteria and a drive system for agitating the biological support while it is it is immersed in the reaction liquor that is to be treated within the vessel. The drive system causes the biological support to oscillate with a reciprocating or rectilinear motion and the drive system is preferably located either outside the vessel or within a portion of the vessel that is above the level of reaction liquor present therein.

This invention relates to a biological functional fluorescent magnetic nanometer particle and preparation method and application. The particle has core-shell structure, including: fluorescent material and magnetic material of kernel layer; the shell layer formed by fluorescent transparent material; and the modified layer above the shell layer surface with organic functional group. Through reverse microemulsion, it cans synthesis nanometer particles with both fluorescent and magnetic function, and process chemical modification on its surface to make biological functional magnetic nanometer particles. The nanometer particles can be used for biological marking, biological imaging and biological separation, etc.

The invention discloses an anaerobic ammonium oxidation biochemical treatment method for coking waste water, and belongs to the field of coking waste water treatment. A water inlet channel, a hypoxiapreconditioning zone, an MBBR front aerobic zone, a prepositive anoxic zone, an MBBR nitrosation aerobic zone, an MBBR anaerobic ammonium oxidation zone, an MBBR rear aerobic zone, a sludge postpositive anoxic zone, a postpositive sludge aerobic zone and an advective condensation zone are involved; the prepositive anoxic zone is communicated with the MBBR nitrosation aerobic zone through a squarewater passing hole formed in the upper side of one side end of a separation wall arranged between the prepositive anoxic zone and the MBBR nitrosation aerobic zone, and a stirring device is arranged in the prepositive anoxic zone; the MBBR nitrosation aerobic zone is communicated with the MBBR anaerobic ammonium oxidation zone through a square water passing hole formed in the upper side of one side end of a separation wall arranged between the MBBR nitrosation aerobic zone and the MBBR anaerobic ammonium oxidation zone; the MBBR nitrosation aerobic zone is communicated with the MBBR rear aerobic zone through a square water passing hole formed in the upper side of one side end of a separation wall arranged between the MBBR nitrosation aerobic zone and the MBBR rear aerobic zone. According to the method, prepositive anaerobic ammonium oxidation and postpositive denitrification nitrogen removal are combined to ensure that the total nitrogen mount of the water discharged to an external environment after coking waste water treatment is conducted reaches standards.

The invention relates to an anoxic denitrification fluidized bed treatment apparatus, which comprises a reactor main body (4), an ultrasonic wave oscillator (containing a vibration plate (19)) and a nitrogen generation system (5), wherein the water inlet (20) of a circulation pump (3) is positioned in a filtration screen (17) near a tower head, the pore size of the filtration screen (17) is slightly smaller than the diameter of a biological filler so as to effectively prevent the biological filler in the reactor from entering a circulation system, the ultrasonic wave oscillator is arranged nearby, sludge originally attached to the filtration screen (17) is dispersed and peeled off through the direct action and the indirect action such as the cavitation effect, the direct inflow effect andthe acceleration effect of the ultrasonic wave oscillator on liquids and dirt so as to clean the filtration screen (17) to make the circulation system operate stably for a long time, and with a waterflow distributor (9) arranged on the bottom portion of the reactor main body (4), the problems of non-uniform influent distribution of the reactor main body and the like can be effectively solved, theshort circuit of water flow can be avoided, and the removal load of organic matters and nitrates can be improved.

The invention discloses an up-flow anaerobic biological reaction/precipitator used for wastewater treatment. The up-flow anaerobic biological reaction/precipitator is rectangular in shape; an integrated structure of the up-flow anaerobic biological reaction/precipitator comprises a reaction zone, a water distribution zone, a settling zone, a water inlet and backflow system, a water outlet system, and a sludge and gas discharge system; the reaction zone is arranged at the lower part of the up-flow anaerobic biological reaction/precipitator; the settling zone is arranged at the middle of the upper part of the up-flow anaerobic biological reaction/precipitator; the bottom of the settling zone is provided with a water distribution/sludge backflow system; the water distribution zone is arranged between the reaction zone and the settling zone; the water inlet and backflow system is arranged in the reaction zone; and the water outlet system is arranged at the upper part of the up-flow anaerobic biological reaction/precipitator and on the diagonal corners. Up-flow reaction conditions can be obtained using the up-flow anaerobic biological reaction/precipitator, and are beneficial for biological reaction process mass transferring; biological reaction efficiency is increased; after biological reaction, wastewater is subjected to precipitation in the integrated settling zone, sludge obtained via precipitation is delivered back to the reaction zone, and it is beneficial for maintenance of microbial biomass.

The invention discloses a method for improving the expression quantity of a pichia pastoris foreign recombinant protein by using mercaptan peroxidase as a cofactor aiming at improving the preparation capability of the pichia pastoris foreign recombinant protein, belonging to the field of biochemical engineering. The method comprises the steps of connecting a mercaptan peroxidase gene with an inducible promoter or a constitutive promoter from pichia pastoris or saccharomyces cerevisiae to construct a gene expression vector, and transferring the gene expression vector to a methanol induced pichia pastoris strain for expressing the foreign recombinant protein to perform co-expression or over-expression, wherein the mercaptan peroxidase gene serving as a global control gene of oxidation reduction stress response improves the expression quantity of the foreign recombinant protein in the methanol induced pichia pastoris under the control effects of the promoter of different strengths and the transcriptional level of a gene copy number. The invention provides a new method for improving the capability of producing recombinant protein by cells and the bio-reaction efficiency, which has an important value for strengthening a biotechnology process.

The invention discloses an anoxic fluidized bed device for treating high-concentration nitrate-nitrogen wastewater. The anoxic fluidized bed device comprises a feeding tank, an anoxic fluidized bed, anitrogen generator system and a nitrogen outlet distributor, wherein the anoxic fluidized bed comprises a filling material area, a transition section and a water outlet area which are sequentially arranged from bottom to top; a three-phase separator is arranged in the water outlet area; a water inlet is arranged at the bottom end of the anoxic fluidized bed; a water flow distributor is arranged above the water inlet and below the filling material area; a water outlet is arranged at the upper part of the water outlet area; the feeding tank is connected with the water inlet of the anoxic fluidized bed through a feeding pipe; the nitrogen generator system is connected with the nitrogen outlet distributor through a gas inlet pipe; the nitrogen outlet distributor is located in the filling material area of the anoxic fluidized bed; and the position of the nitrogen outlet distributor in the anoxic fluidized bed is adjustable. The device provided by the invention realizes accurate demolding in a directional positioning way through arrangement of the position of the nitrogen outlet distributor according to needs.

The invention discloses a method for treating high-concentration nitrate wastewater. The method includes the following steps: S1, adding a carbon source and nitrogen and phosphorus nutrients into thenitrate wastewater, and adjusting the pH of the wastewater to 2-4 to obtain pretreated wastewater; and S2, mixing the pretreated wastewater and circulating water, introducing the obtained mixed waterinto an anoxic expanded bed reactor to be in contact with a biological filler, and performing a denitrification reaction to obtain denitrified wastewater, wherein the circulating water is a product obtained by the denitrification reaction of the pretreated wastewater and the biological filler. The cost of treating the nitrate wastewater by using the method is lower, and the method can continuouslytreat the high-concentration nitrate (3000-35000 mg/L) wastewater, and has high treatment efficiency.