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Fluorescent nano-particle with surface biological function, its production and use

A biological functionalization and fluorescent nanotechnology, which is applied in biological testing, fluorescence/phosphorescence, material inspection products, etc., can solve the problems that fluorescent nanomaterials have the function of binding biomolecules at the same time, and achieve trace detection and trace reaction. , smooth surface and uniform particle distribution

Inactive Publication Date: 2007-01-17
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a core-shell type fluorescent nanoparticle with surface biofunctionalization and its preparation method and application, so as to overcome the fluorescent substance in the prior art that is only modified on the surface of nanomaterials and cannot make fluorescent nanomaterials Simultaneously have the defect of biomolecular binding function

Method used

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  • Fluorescent nano-particle with surface biological function, its production and use
  • Fluorescent nano-particle with surface biological function, its production and use
  • Fluorescent nano-particle with surface biological function, its production and use

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Preparation Method 1 of Core-shell Fluorescent Nanoparticles

[0042] Mix isopropanol and water uniformly at a ratio of 5:1, and sonicate for 5 minutes. Weigh 0.1-0.8 mg of fluorescent substance fluorescein isothiocyanate FITC, configure it into an aqueous solution, and process it ultrasonically for 10 min. Pour the upper clear layer into a three-necked flask, and keep stirring to keep it in a dispersed state. Take 1mL of concentrated ammonia water and slowly add it into the constantly stirring solution system. Then take 1-3 mL of tetraethyl orthosilicate, and also slowly add it into the constantly stirring solution system. The system was reacted at room temperature for 3 to 5 hours. The reaction product was poured out, and the particles were washed 3 to 5 times with twice distilled water. The cleaned particles were vacuum-dried for 5 hours at a temperature of 20-50° C., and the particles were collected for future use. From figure 1 We can see that the silica has ...

Embodiment 2

[0044] Preparation Method 2 of Core-shell Fluorescent Nanoparticles

[0045] Mix TritonX-100, n-hexanol and cyclohexane uniformly at a ratio of 1:2:5 to form a transparent and stable microemulsion system. Put the above-mentioned microemulsion system in ultrasonic treatment for 30-60 minutes, add 0.5 mg of fluorescent substance fluorescein isothiocyanate FITC to it, use ultrasonic treatment for 6 minutes, take out the supernatant liquid and pour it into a three-necked flask, stir for 30 minutes to make It's even. Take 1mL of concentrated ammonia water and dilute it with 2mL double-distilled water, slowly add it into the microemulsion under constant stirring after 30 minutes, and keep stirring for 30 minutes to disperse the ammonia water evenly in the microemulsion. After 1 hour, 1-3 mL of tetraethyl orthosilicate was added dropwise to the microemulsion, while stirring continuously for 10 hours, and the temperature of the system was kept between 15-30°C. Add acetone to the sys...

Embodiment 3

[0047] Amino modification on the surface of fluorescent nanoparticles

[0048] Take 20 mg of the fluorescent nanoparticles prepared in Example 1 or 2, add 30-50 mL of methanol and glycerol in a 5:3 mixture, and use ultrasonic treatment for 20-60 minutes; weigh 1-3 mL of AEAPS [N-(2-aminoethyl)-3-aminopropyltrimethoxysilane], ultrasonic treatment for 10 to 60 minutes; mix the two solutions evenly, react at 60°C for 5 hours, and then take out The particles were washed with methanol for 3 times, followed by vacuum drying at 40-80° C. for 2 hours, and the particles were collected to obtain biofunctionalized fluorescent nanoparticles with amino groups modified on the surface.

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Abstract

This invention relates to a preparation method and its application of nuclear shell fluorescence nanometer particle whose surface living things is functional. The particle has nuclear shell structure, fluorescence material is hit in the inner core; the outer shell is composed of fluorescence transparency material; and there is embellishing organic function group in the outer covering. The nanometer particle is formed by the reversal tiny emulsion method, and doing chemical modification in the surface to make it to be biology functional nanometer particle. The nanometer particle has important application prospect in the fields such as cell biology, ultramicrochemistry, within the AMD chemistry, living things big molecule detecting and medical in-vivo diagnosing.

Description

Background technique [0001] In recent years, biological detection technology has developed rapidly, among which the development of ultra-sensitive fluorescence detection technology provides conditions for the study of biomolecules in complex environments. The establishment of ultra-sensitive fluorescence detection technology depends on the successful development of non-toxic and biocompatible luminescent materials. [0002] Organic fluorescent substances are classic fluorescent substances. Although organic fluorescent substances have their shortcomings due to their inherent properties, such as fluorescence efficiency problems, etc., compared with current inorganic fluorescent substances, organic fluorescent substances still have a wide range of applications. Such as applied to flow cytometry, applied to antibody labeling and so on. [0003] Biofunctional materials have always been a hot field in the field of materials research. In biofunctional materials, nanomaterials have a...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/64
Inventor 沈鹤柏周丽佳陈伟朱龙章
Owner SHANGHAI NORMAL UNIVERSITY
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