Fluorescent nano-particle with surface biological function, its production and use

A biological functionalization and fluorescent nanotechnology, which is applied in biological testing, fluorescence/phosphorescence, material inspection products, etc., can solve the problems that fluorescent nanomaterials have the function of binding biomolecules at the same time, and achieve trace detection and trace reaction. , smooth surface and uniform particle distribution

Inactive Publication Date: 2007-01-17
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a core-shell type fluorescent nanoparticle with surface biofunctionalization and its preparation method and application, so as to

Method used

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  • Fluorescent nano-particle with surface biological function, its production and use
  • Fluorescent nano-particle with surface biological function, its production and use
  • Fluorescent nano-particle with surface biological function, its production and use

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0040] Example 1

[0041] Preparation method 1 of core-shell fluorescent nanoparticles

[0042] Mix isopropanol and water uniformly in a ratio of 5:1, and ultrasonicate for 5 minutes. Weigh 0.1-0.8 mg of the fluorescent substance fluorescein isothiocyanate FITC, configure it into an aqueous solution, and ultrasonically treat it for 10 minutes. Take the supernatant and pour it into a three-necked flask with constant stirring to keep it in a dispersed state. Take 1 mL of concentrated ammonia water and slowly add it to the continuously stirred solution system. Then take 1-3 mL of tetraethyl orthosilicate and slowly add it to the continuously stirred solution system. The system reacts at room temperature for 3 to 5 hours. The reaction product is poured out, and the particles are washed 3 to 5 times with double distilled water. The cleaned particles are vacuum dried at a temperature of 20-50° C. for 5 hours, and the particles are collected for later use. From figure 1 We can see that ...

Example Embodiment

[0043] Example 2

[0044] Preparation method 2 of core-shell fluorescent nanoparticles

[0045] TritonX-100, n-hexanol and cyclohexane are uniformly mixed in a ratio of 1:2:5 to form a transparent and stable microemulsion system. Put the above microemulsion system under ultrasonic treatment for 30-60 minutes, add 0.5 mg of fluorescein isothiocyanate FITC to it, take out the supernatant liquid after ultrasonic treatment for 6 minutes and pour it into a three-necked flask, stir for 30 minutes to make It's even. Dilute 1 mL of concentrated ammonia water with 2 mL of double distilled water, slowly add it to the continuously stirred microemulsion after 30 minutes, and continue stirring for 30 minutes to make the ammonia water evenly dispersed in the microemulsion. After 1 hour, 1-3 mL of tetraethyl orthosilicate was added dropwise to the microemulsion while continuously stirring for 10 hours, and the temperature of the system was maintained at 15-30°C. Add acetone to the system to prec...

Example Embodiment

[0046] Example 3

[0047] Modification of Amino Groups on the Surface of Fluorescent Nanoparticles

[0048] Take 20 mg of the fluorescent nanoparticles prepared in Example 1 or 2, and add 30-50 mL of methanol and glycerol in a 5:3 ratio mixed solution, and ultrasonic treatment for 20-60 minutes; weigh 1 to 3 mL AEAPS [N-(2-Aminoethyl)-3-aminopropyltrimethoxysilane], ultrasonic treatment for 10-60 minutes; mix the two solutions uniformly, react at 60°C for 5 hours, and then take it out The particles were washed 3 times with methanol, and then vacuum dried at 40-80° C. for 2 hours. The particles were collected to obtain biologically functionalized fluorescent nanoparticles modified with amino groups on the surface.

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Abstract

This invention relates to a preparation method and its application of nuclear shell fluorescence nanometer particle whose surface living things is functional. The particle has nuclear shell structure, fluorescence material is hit in the inner core; the outer shell is composed of fluorescence transparency material; and there is embellishing organic function group in the outer covering. The nanometer particle is formed by the reversal tiny emulsion method, and doing chemical modification in the surface to make it to be biology functional nanometer particle. The nanometer particle has important application prospect in the fields such as cell biology, ultramicrochemistry, within the AMD chemistry, living things big molecule detecting and medical in-vivo diagnosing.

Description

Background technique [0001] In recent years, biological detection technology has developed rapidly, among which the development of ultra-sensitive fluorescence detection technology provides conditions for the study of biomolecules in complex environments. The establishment of ultra-sensitive fluorescence detection technology depends on the successful development of non-toxic and biocompatible luminescent materials. [0002] Organic fluorescent substances are classic fluorescent substances. Although organic fluorescent substances have their shortcomings due to their inherent properties, such as fluorescence efficiency problems, etc., compared with current inorganic fluorescent substances, organic fluorescent substances still have a wide range of applications. Such as applied to flow cytometry, applied to antibody labeling and so on. [0003] Biofunctional materials have always been a hot field in the field of materials research. In biofunctional materials, nanomaterials have a...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/64
Inventor 沈鹤柏周丽佳陈伟朱龙章
Owner SHANGHAI NORMAL UNIVERSITY
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