Biological functional fluorescence magnetic particle and its preparing method and application

A technology of magnetic nanoparticles and biological functionalization, applied in the field of nanomaterials in the biological field, can solve the problems that nanomaterials cannot have biological functions at the same time, and achieve the effect of simple and easy micro-reaction, reducing the total amount and improving efficiency

Inactive Publication Date: 2007-05-23
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved in the present invention is to provide a biologically functionalized fluorescent magnetic nanoparticle and its preparation method and application, so as to overcome the defect in the prior art that fluorescent substances are only modified on the surface of nanomaterials and cannot make nanomaterials have biological functions at the same time

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  • Biological functional fluorescence magnetic particle and its preparing method and application
  • Biological functional fluorescence magnetic particle and its preparing method and application
  • Biological functional fluorescence magnetic particle and its preparing method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Preparation method of inner core layer of magnetic nanoparticles

[0043] The core layer of magnetic particles was prepared by improved chemical co-precipitation, the specific method is as follows: prepare FeSO 4 ·7H 2 O and FeCl 3 ·6H 2 O mixed solution and NaOH solution. In a mixed solution of iron salts, Fe 2+ The ion concentration is 0.1~0.2mol / L, Fe 3+The concentration of ions is 0.1-0.3 mol / L, and the concentration of NaOH solution is 2-3 mol / L. Under vigorous stirring, the NaOH solution whose volume is half of the volume of the mixed salt solution was slowly added dropwise into the mixed salt solution. Aging the obtained solid precipitate at 40°C to 60°C for 12 hours, washing the precipitate several times with double distilled water, filtering and drying at 40°C to 80°C for 24 hours, and grinding it in an agate mortar Get the product.

Embodiment 2

[0045] Preparation Method 1 of Core-shell Fluorescent Magnetic Nanoparticles

[0046] Mix TritonX-100, n-hexanol and cyclohexane uniformly at a ratio of 1:2:5 to form a transparent and stable microemulsion system. Put the above-mentioned microemulsion system in ultrasonic treatment for 30-60 minutes, add 0.5 mg of fluorescent substance fluorescein isothiocyanate FITC to it, and then add 0.5 g of γ-Fe 2 o 3 After 6 minutes of ultrasonic treatment, the supernatant was taken out and poured into a three-necked flask, and stirred for 30 minutes to make it uniform. Take 1mL of a certain concentration of concentrated ammonia water and dilute it with 2mL of double-distilled water, slowly add it into the microemulsion that is constantly stirring after 30 minutes, and continue stirring for 30 minutes to make the ammonia water evenly dispersed in the microemulsion. After 1 hour, 1-3 mL of tetraethyl orthosilicate was added dropwise to the microemulsion, while stirring continuously for ...

Embodiment 3

[0048] Preparation Method 2 of Core-Shell Fluorescent Magnetic Nanoparticles

[0049] Mix isopropanol and water uniformly at a ratio of 5:1, and sonicate for 5 minutes. Weigh 0.1-0.8 mg of one of the fluorescent substances fluorescein isothiocyanate FITC, tetraethylrhodamine, tetramethylrhodamine isothiocyanate, or phycoerythrin, and configure it into an aqueous solution. Then weigh about 1.0g of γ-Fe 2 o 3 Added to the solution system prepared above, ultrasonic treatment for 10min. Pour the upper clear layer into a three-necked flask, and keep stirring to keep it in a dispersed state. Take 1mL of concentrated ammonia water of a certain concentration, and slowly add it into the solution system that is constantly stirring. Then take 1-3 mL of tetraethyl orthosilicate, and also slowly add it into the constantly stirring solution system. The system was reacted at room temperature for 3-5 hours. The reaction product was poured out, and the particles were washed 3-5 times wit...

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Abstract

This invention relates to a biological functional fluorescent magnetic nanometer particle and preparation method and application. The particle has core-shell structure, including: fluorescent material and magnetic material of kernel layer; the shell layer formed by fluorescent transparent material; and the modified layer above the shell layer surface with organic functional group. Through reverse microemulsion, it cans synthesis nanometer particles with both fluorescent and magnetic function, and process chemical modification on its surface to make biological functional magnetic nanometer particles. The nanometer particles can be used for biological marking, biological imaging and biological separation, etc.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and more specifically relates to nanomaterials applied in the field of biology. Background technique [0002] Biofunctional materials have always been a hot field in the field of materials research. In biofunctional materials, nanomaterials have received more and more attention from scientists due to their special physical and chemical properties since the 1980s. Technology research and solving major problems in the field of biology have also become one of the important frontier research fields. [0003] Organic fluorescent substances are classic fluorescent substances. Although organic fluorescent substances have their shortcomings due to their inherent properties, such as fluorescence efficiency problems, etc., compared with current inorganic fluorescent substances, organic fluorescent substances still have a wide range of applications. Such as applied to flow cytometry, applied to antibody ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/569A61K49/08
Inventor 沈鹤柏周丽佳陈伟朱龙章
Owner SHANGHAI NORMAL UNIVERSITY
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