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Albumin angiotensin converting enzyme inhibition peptide and preparation method thereof

A technology of angiotensin and egg white protein, applied in the field of separation and purification of angiotensin-converting enzyme inhibitory peptide and its preparation, and a new angiotensin-converting enzyme inhibitory peptide prepared by biological enzymatic hydrolysis, which can solve the problem of severe side effects, etc. question

Inactive Publication Date: 2010-04-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic antihypertensive drugs have obvious antihypertensive effects, but they have relatively large side effects. People are turning their attention to the research of angiotensin-converting enzyme inhibitory peptides derived from food that are safe and have no side effects.

Method used

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  • Albumin angiotensin converting enzyme inhibition peptide and preparation method thereof
  • Albumin angiotensin converting enzyme inhibition peptide and preparation method thereof
  • Albumin angiotensin converting enzyme inhibition peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Purification of angiotensin-converting enzyme inhibitory peptide RVPSL from egg white proteolysis product

[0028] (1) Enzymolysis of egg white protein to prepare enzymatic hydrolyzate

[0029] A certain amount of egg white protein was prepared into an aqueous solution with a concentration of 5%, and placed in a constant temperature water bath at 90°C for heat denaturation for 10 minutes. Cool to the enzymatic hydrolysis temperature of 40°C, use 0.5moL / L sodium hydroxide solution to adjust the pH to 11, add Alcalase 2.4L, which accounts for 5% of the egg white protein mass, for hydrolysis, keep stirring during the hydrolysis process, and keep adding an appropriate concentration Sodium hydroxide solution to maintain the pH value within the range of 11 (±0.05), after hydrolysis for 20 hours, the enzyme was inactivated at 80°C for 10 minutes, centrifuged at 1000g for 10 minutes, spray-dried, and stored for later use;

[0030] (2) Purification of angiotensin-conv...

Embodiment 2

[0036] Example 2 As described in Example 1, the difference is that the amount of Alcalase 2.4L used to prepare the enzymolyzate from egg white protein is 1% of the egg white protein, the pH value of the enzymolysis is 8, and the enzymolysis time is 2 hours. 10min, freeze-dried, and stored for future use.

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Abstract

The invention provides an albumin angiotensin converting enzyme inhibition peptide and a preparation method thereof, belonging to the biotechnology field. A new active peptide with angiotensin converting enzyme inhibition function is separated and purified from albumin zymolyte and has a sequence of RVPSL. The preparation method comprises the steps of: carrying out the enzymolysis on albumin to prepare the zymolyte, purifying the albumin angiotensin converting enzyme inhibition peptide, identifying the structure of the albumin angiotensin converting enzyme inhibition peptide, synthesizing the albumin angiotensin converting enzyme inhibition peptide and checking the activity of the albumin angiotensin converting enzyme inhibition peptide. The invention can be used for preparing antihypertensive drugs and functional foods assisting blood pressure reduction.

Description

technical field [0001] The invention relates to a separation and purification of angiotensin-converting enzyme inhibitory peptide from egg white proteolysis products and a preparation method thereof, in particular to a new angiotensin-converting enzyme inhibitory peptide prepared by a biological enzymatic hydrolysis method, which belongs to biotechnology field. Background technique [0002] Hypertension is a common cardiovascular disease with a high incidence and is often accompanied by functional or organic changes in the heart, blood vessels, lungs, and kidneys. Complications and an important risk factor for stroke, promotion of atherosclerosis, and coronary heart disease. Hypertension is currently the leading cardiovascular disease in my country. There are about 160 million hypertensive patients in China, and the treatment of hypertension is imminent. Synthetic antihypertensive drugs have obvious antihypertensive effect, but they have relatively large side effects. Peop...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06A61P9/12
Inventor 刘静波于志鹏赵文竹于一丁
Owner JILIN UNIV
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