Method for preparing low blood sugar starch by enzymatic modification
A technology of low blood sugar and starch, applied in the field of starch deep processing, can solve the problems of single RS or SDS, SDS products are not on the market, and achieve the effect of good product quality, excellent product quality and good thermal stability.
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Embodiment 1
[0025] Weigh 1 g of raw starch to make a starch milk solution with a concentration of 1%, and then cook it at 100°C for 2 minutes to make it completely gelatinized; after the starch paste is cooled to room temperature, add 50 mL of Tris-HCl buffer solution and Sucrase made into 100mM sucrose solution and 0.5U / g starch; at 30°C, pH 6.5, constant temperature reaction for 24h; after inactivating enzyme treatment, the enzymolyzed product was dried and pulverized to obtain rich slow-digestible starch Low glycemic starch derivatives with resistant starch. It has been determined that the contents of slow digestible starch and resistant starch in the product are respectively increased by 30% and 13% compared with the original starch, and the ratio range of short chain (DP13) in the chain structure is 0.47.
Embodiment 2
[0027] Weigh 1g of raw starch to make a starch milk solution with a concentration of 12%, and then cook it at 80°C for 25min to make it completely gelatinized; after the starch paste is cooled to room temperature, add 70mL Tris-HCl buffer solution and 500mM sucrose solution and 40U / g starch sucrase; at 45°C and pH 7.3, constant temperature reaction for 2h; A low glycemic starch derivative of resistant starch. It has been determined that the contents of slow digestible starch and resistant starch in the product are respectively increased by 19% and 15% compared with the original starch, and the ratio range of short chain (DP13) in the chain structure is 0.29.
Embodiment 3
[0029] Weigh 1g of raw starch to make a starch milk solution with a concentration of 30%, and then cook it at 90°C for 10 minutes to make it completely gelatinized; after the starch paste is cooled to room temperature, add 100mM Tris-HCl buffer solution and sucrose to make it 1000mM sucrose solution and 95U / g starch sucrase; at 50 ℃, pH 8.0 conditions constant temperature reaction 48h; A low glycemic starch derivative of resistant starch. It has been determined that the contents of slow digestible starch and resistant starch in the product are respectively increased by 24% and 19% compared with the original starch, and the ratio of short chain (DP13) in the chain structure is 0.13.
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