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Preparation method of test paper for detecting alcohol content in saliva and test paper prepared thereby

A test strip and alcohol technology, which is used in biological testing, material inspection products, and material analysis by observing the impact on chemical indicators, can solve the problem of inability to quickly detect and determine on-site, and achieve convenient on-site testing, simple preparation process, Avoid oxidative effects

Inactive Publication Date: 2006-09-27
丁国兴
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  • Description
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Problems solved by technology

[0004] The known technical problem is that in traffic law enforcement, it is impossible to quickly detect and determine whether the person under test has drunk alcohol and how much alcohol he has drunk

Method used

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  • Preparation method of test paper for detecting alcohol content in saliva and test paper prepared thereby
  • Preparation method of test paper for detecting alcohol content in saliva and test paper prepared thereby
  • Preparation method of test paper for detecting alcohol content in saliva and test paper prepared thereby

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Embodiment Construction

[0034] The specific implementation, structure, features and effects provided by the present invention will be described in detail below in conjunction with the accompanying drawings and preferred embodiments.

[0035] A method for preparing a test paper for detecting the alcohol content of saliva. The pure cotton pulp filter paper with a pure cotton pulp greater than 95-98% and free of bleaching substances and any strong oxidizing substances is selected as the substrate carrier of the test paper, which is called the test paper body 1. Using solid chemical technology, 3.3'.5.5'-Tetramethylbenzidine (TMB), phosphate buffer (Buffer), horseradish peroxidase (HRP), alcohol oxidase (A10D) and bovine serum albumin ( BSA) successively add each reagent on the test paper, step by step vacuum drying, its specific steps are:

[0036] Take the aforementioned test paper body 5×5 square millimeters,

[0037] Add 3.3'.5.5'-Tetramethylbenzidine (TMB) solution, take the 3.3'.5.5'-Tetramethylbe...

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Abstract

This invention relates to a preparation method of test paper for detecting alcohol content in saliva and test paper prepared thereby. Wherein, using pure cotton pulp filter paper as the bottom carrier of test paper; with solid chemistry technique, adding in turn the tetramethyl benzidine, phosphate buffer, HRP, alcohol oxidase and BSA on the paper, and multiple-step vacuum drying. This invention is safe and reliable for test, can read result directly with color-matching card, and has well repeatability for wide application.

Description

technical field [0001] The invention belongs to the technical field of detecting saliva ethanol, in particular to a test paper for detecting alcohol content in saliva, which is especially suitable for on-site testing. Background technique [0002] The social application of alcohol detection is very extensive and very important, and on-site detection of alcohol content in the body of motor vehicle drivers is particularly important. It is a common practice in the world to analyze and determine the responsibility of traffic accidents based on the alcohol concentration in blood or saliva. In practice, taking blood samples to test the alcohol content always has potential health and safety risks; There are also many problems in operation, such as the authenticity of urine samples, etc., so people prefer the technical application of taking saliva for alcohol testing. [0003] The known technology, such as the published Chinese patent application 03128880...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N33/50G01N21/78
Inventor 丁国兴
Owner 丁国兴
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