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Reagent strip for detecting alcohol content in saliva, preparation method of reagent strip and kit

A reagent strip and alcohol technology, applied in the field of reagent strips, preparation methods and kits for detecting alcohol content in saliva, can solve problems such as poor accuracy of detection results, and achieve excellent stability, good solubility, and uniform color development. Effect

Active Publication Date: 2018-01-19
NANTONG EGENS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the first technical problem to be solved in the present invention is to overcome the problem of low stability of the existing reagent strips for detecting alcohol content in saliva, thereby providing a stable reagent for detecting alcohol content in saliva strip; the second technical problem to be solved by the present invention is to overcome the problem that the existing reagent strips for detecting the alcohol content in saliva cause the poor accuracy of the detection results due to the use of TMB as the easy crystallization of the chromogenic agent, thereby providing A reagent strip for detecting alcohol content in saliva with high detection result accuracy; further, the present invention also provides a preparation method of the above reagent strip and a kit containing it

Method used

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  • Reagent strip for detecting alcohol content in saliva, preparation method of reagent strip and kit
  • Reagent strip for detecting alcohol content in saliva, preparation method of reagent strip and kit
  • Reagent strip for detecting alcohol content in saliva, preparation method of reagent strip and kit

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Effect test

Embodiment 1

[0041] The present embodiment provides a kind of preparation method of Luofen base derivative (I), and its reaction principle is:

[0042]

[0043] 106.5g of 4,4'-bis(dimethyl)phenyl ester, 59g of syringaldehyde, 111g of ammonium acetate and 325mL of acetic acid were respectively added into a 1000mL round bottom flask, and stirred for 3h. After the reaction, 3.6L of water and 1.2L of dichloromethane were added, the organic phase was separated, the water phase was extracted once with 200mL of dichloromethane solution, the organic phases were combined, and concentrated to dryness.

Embodiment 2

[0045] The present embodiment provides a kind of reagent strip that is used to detect the alcohol content in saliva, and it is made up of the filter paper and milky white PET board that do not contain bleaching substances and strong oxidizing substances, and the reagent composition on the filter paper is: Lofen base derivative ( 1) Chromogen, ethanol oxidase and horseradish peroxidase, bovine serum albumin and sodium alginate, the mass ratio of described bovine serum albumin and sodium alginate is 80:1, and its preparation process is specifically:

[0046] (1) Preparation of base liquid:

[0047] Add 20 g of bovine serum albumin and 0.25 g of sodium alginate into 100 mL of 0.2 mol / L sodium dihydrogen phosphate / disodium hydrogen phosphate aqueous buffer solution with a pH of 7.4, and stir until completely dissolved.

[0048] (2) preparation of alcohol oxidase and horseradish peroxidase solution:

[0049] Add 80U of alcohol oxidase and 300U of horseradish peroxidase into 10mL b...

Embodiment 3

[0061] The present embodiment provides a kind of reagent strip that is used to detect the alcohol content in saliva, and it is made up of filter paper and white PET board that do not contain bleaching substances and strong oxidizing substances, and the reagent composition on the filter paper is: Lofen base derivative ( 1) Chromogen, ethanol oxidase and horseradish peroxidase, bovine serum albumin and sodium alginate, the mass ratio of described bovine serum albumin and sodium alginate is 70:1, and its preparation process is specifically:

[0062] (1) Preparation of base liquid:

[0063] Add 15 g of bovine serum albumin and 0.21 g of sodium alginate into 100 mL of 0.2 mol / L sodium dihydrogen phosphate / disodium hydrogen phosphate aqueous buffer solution with a pH of 7.5, and stir until completely dissolved.

[0064] (2) preparation of alcohol oxidase and horseradish peroxidase solution:

[0065] Add 56U of alcohol oxidase and 450U into 10mL base solution, mix well and set aside...

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Abstract

The invention belongs to the technical field of detection of alcohol in saliva and discloses a reagent strip for detecting the alcohol content in saliva, a preparation method of the reagent strip anda kit. The reagent strip contains bovine serum albumin and sodium alginate. The bovine serum albumin has good solubility, can be dissolved into a buffer solution at the normal temperature and can be uniformly mixed with alcohol oxidase and a horse radish peroxidase solution, so that the decomposition and nonspecific adsorption of enzyme are prevented; and sodium alginate has viscidity and can be tightly adsorbed to the surface of the bovine serum albumin after being mixed with the bovine serum albumin. The bovine serum albumin and sodium alginate are commonly used as an additive of alcohol oxidase and horse radish peroxidase, so that the oxygen and moisture in air are effectively isolated, the decomposition and nonspecific adsorption of enzyme are prevented, the false positive phenomenon generated in a detection process of a product is avoided, and the product has excellent stability.

Description

technical field [0001] The invention belongs to the technical field of alcohol detection in saliva, and in particular relates to a reagent strip, a preparation method and a kit for detecting the alcohol content in saliva. Background technique [0002] With the rapid development of the automobile industry and the rapid improvement of people's living standards, motor vehicles have increased sharply, and at the same time traffic accidents have also occurred frequently. The World Health Organization accident survey in 2008 showed that about 50-60% of traffic accidents were related to drunk driving. In China, there are tens of thousands of traffic accidents caused by drunk driving every year, and more than 50% of the fatal accidents are related to drunk driving. In this sense, alcohol is becoming a "road killer". To judge whether it belongs to drunk driving, it is necessary to test the alcohol content in the driver's body. my country's national standard GB19522-2010 "Threshold...

Claims

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Application Information

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IPC IPC(8): G01N33/98G01N21/78
Inventor 顾飞欧卫军张娟张小飞
Owner NANTONG EGENS BIOTECH
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