Oral delivery of enzymes by nanocapsules for targeted metabolism of alcohol or toxic metabolites

A nanocomposite, alcohol technology, used in capsule delivery, nanomedicine, microcapsules, etc., can solve problems such as impaired motor coordination

Inactive Publication Date: 2014-05-07
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, impaired CNS inhibition and motor coordination, as well

Method used

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  • Oral delivery of enzymes by nanocapsules for targeted metabolism of alcohol or toxic metabolites
  • Oral delivery of enzymes by nanocapsules for targeted metabolism of alcohol or toxic metabolites
  • Oral delivery of enzymes by nanocapsules for targeted metabolism of alcohol or toxic metabolites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Also shown in Example 1 is an enzyme nanocomplex n (AOx-Cat) containing alcohol oxidase (AOx) and catalase (Cat) and the potential of said nanocomplex as an alcohol detoxifier. Both in vitro and in vivo experiments demonstrated the remarkable role of n(AOx-Cat) in metabolizing alcohol and attenuating alcohol-induced liver injury while converting the toxic by-product H 2 O 2 release is minimized. Therefore, the experimental data clearly demonstrate that the enzyme nanocomplex (EN) of the present disclosure achieves improved catalytic efficiency and increased stability, and at the same time can reduce intermediates that may be toxic to the environment (such as H 2 O 2 ) release. Thus, one embodiment of the present disclosure provides a nanocomplex comprising two or more enzymes, said nanocomplex comprising a first enzyme and a second enzyme and a polymer network anchoring anchored to at least one of the enzymes or alternatively at least two or three or four or five or...

Embodiment

[0191] From the foregoing description it will be apparent that the invention described herein can be altered and modified to adapt it to various usages and conditions. Such embodiments are also within the scope of the following claims.

[0192] The recitation of a listing of elements in any variable definition herein includes definitions of that variable as any single element or combination (or subcombination) of listed elements. The recitation of an embodiment herein includes that embodiment as any single embodiment or in combination with any other embodiment or portion thereof.

[0193] Terms listed in the singular tense also include the plural unless the context dictates otherwise.

[0194] The following disclosed examples are provided to illustrate the invention without limiting its scope. Other variations of the invention will be apparent to those skilled in the art and are covered by the appended claims. All publications, databases, and patents cited herein (e.g., U.S...

Embodiment 2

[0224] Example 2: Illustrative methods and materials for making and using artificial enzyme nanocomplexes (EN) with synergistic functions

[0225] This example provides more details of the materials, methods and results discussed in Example 1.

[0226] 1. Materials

[0227] Unless otherwise stated, reagents and solvents were purchased from Sigma-Aldrich (St. Louis, MO) and used as received without further purification. HeLa cells were purchased from the American Type Culture Collection (ATCC). Dulbecco's Modified Eagle Medium (DMEM) growth medium and penicillin / streptomycin were obtained from Invitrogen (Carlsbad, CA). Fetal Bovine Serum (FBS) was obtained from Lonza Walkerrsville Inc (Walkerrsville, MD). CellTitra Blue cell viability kit was purchased from Promega (Madison, WI). N-(3-Aminopropyl)ethylacrylamide hydrochloride used to make the enzyme nanocomplex was purchased from Polymer Science, Inc. Monothio-N-hydroxy-succinimide based nanoparticles were purchased from ...

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Abstract

The invention disclosed herein includes nanocomplexes that are designed include enzymes that have complementary functional attributes and methods for using these nanocomplexes. Illustrative examples include nanocomplexes that comprise both an alcohol oxidase enzyme as well as a catalase enzyme. These nanocomplexes can be used in methods designed to lower blood alcohol levels in vivo, and/or to break down the toxic byproducts of alcohol metabolism. Consequently these nanocomplexes can be used to treat a variety of conditions resulting from the consumption of alcohol, including for example, acute alcohol intoxication.

Description

[0001] Citations to Related Applications [0002] This application claims under Section 119(e) of the United States Patent Act a co-pending U.S. Provisional Patent Application Serial titled "ORAL DELIVERY OF ENZYMES BY NANOCAPSULES FOR TARGETED METABOLISM OF ALCOHOL OR TOXIC METABOLITES," filed July 6, 2011 61 / 504,975 and the benefit of co-pending U.S. Provisional Patent Application Serial No. 61 / 529,767, filed August 31, 2011, entitled "MULTIPLE-ENZYME NANOCOMPLEXES," the contents of each of which are incorporated by reference In this article. [0003] Statement Regarding Government Funding [0004] This invention was made with government support under Grant Numbers R01AA18612 and R01AA018846 awarded by the National Institutes of Health and the National Institute on Alcohol Abuse and Alcoholism. The government has certain rights in this invention. technical field [0005] The present disclosure relates to methods of administering enzyme nanocomplexes designed to metabolize...

Claims

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Application Information

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IPC IPC(8): A61K9/48A61K9/50A61K47/48A61K47/30A61K48/00
CPCB82Y5/00A61K47/549A61K38/44A61K38/443A61P39/02C12N11/18A61K47/64
Inventor O.O.杨Y.鲁C.纪闫明刘阳
Owner RGT UNIV OF CALIFORNIA
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