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Electrochemical ethyl alcohol sensor based on ethyl alcohol oxidase and preparing method thereof

A technology of alcohol oxidase and sensors, which is applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve problems such as damage and unobvious benefits, and achieve the effects of accurate test results, high selectivity and sensitivity, and short response time

Inactive Publication Date: 2016-01-06
SHENZHEN METERAGE SCI & TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Patents JP2007225444, JPH0196552, etc. use enzyme technology to prepare fuel cell-type alcohol sensors. The problems faced by this sensor are larger than those of the above-mentioned patents. This is mainly because the two electrodes of the fuel cell-type sensor use opposite electrodes. Higher activity electrochemical catalysts, which makes the benefits of using enzymes less obvious in the first place, and the enzymes themselves are more easily destroyed

Method used

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  • Electrochemical ethyl alcohol sensor based on ethyl alcohol oxidase and preparing method thereof

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Embodiment 1

[0027] An electrochemical ethanol sensor based on alcohol oxidase, comprising a substrate and a supporting electrolyte layer 4 arranged on the substrate, a counter electrode 5, a reference electrode 7 and a working electrode 3, the working electrode 3 and the counter electrode 5 and the reference electrode 3 The ratio electrodes 7 are all separated, and the working electrode 3 is covered with an alcohol oxidase layer 1, and the alcohol oxidase layer 1 is made of an electrically insulating biologically active material on which the alcohol oxidase is carried. ) obtained on an electrically insulating bioactive layer, an auxiliary diffusion layer 2 is provided between the working electrode 3 and the alcohol oxidase layer 1, and an auxiliary diffusion layer 2 is arranged between the alcohol oxidase layer 1 and the working electrode 3. A partially interpenetrating network structure is formed and tightly combined; ethanol generates acetaldehyde under the action of alcohol oxidase, and...

Embodiment 2

[0037] A preparation method of the described electrochemical ethanol sensor based on ethanol oxidase, the method comprises the following steps: 1. preparation of electrodes: a working electrode, a counter electrode and a reference electrode are arranged at one end of the substrate, and A supporting electrolyte layer is set on the chip; the supporting electrolyte layer 4 is a polymer solid electrolyte layer, so as to avoid the electrolyte from contaminating the alcohol oxidase layer and causing the enzyme layer to be inactivated. More preferably, the polymer solid electrolyte layer includes perfluorosulfonic acid At least one of ion exchange resin, polystyrene sulfonic acid / salt solid electrolyte, sulfonated polypropylene, sulfonated polyether sulfone, potassium polyethylene sulfonate and sodium polyacrylate; ② preparation of auxiliary diffusion layer: in the working electrode printing or spraying the slurry of the auxiliary diffusion layer to obtain the auxiliary diffusion laye...

Embodiment 3

[0039] Embodiment 3: the preparation method of ethanol sensor

[0040] ①A supporting electrolyte layer is set on a porous nitrocellulose substrate with a length of 45mm and a width of 10mm, and a conductive carbon black electrode is printed on one end of the porous nitrocellulose substrate as a working electrode and a counter electrode, and silver- A silver chloride electrode was used as a reference electrode.

[0041] On the reference electrode, between the working electrode and the counter electrode, an electrolyte layer (non-supporting electrolyte) is printed, and its main component is: a polyurethane hydrogel layer containing KCl0.1M.

[0042] ②Print the slurry of the auxiliary diffusion layer on the working electrode, and its main components are emulsion-like polytetrafluoroethylene and auxiliary materials; wherein, emulsion-like polytetrafluoroethylene (surfactant removal) 100g, auxiliary materials are polytetrafluoroethylene 5g of butyl acrylate, 25ml of terpineol, 800...

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Abstract

The invention relates to an electrochemical ethyl alcohol sensor based on ethyl alcohol oxidase and a preparing method thereof. The sensor is provided with three electrodes, the working electrode is covered with an ethyl alcohol oxidase film, ethyl alcohol in sample gas is selectively oxidized into acetaldehyde by means of the ethyl alcohol oxidase, and current generated through oxidization of acetaldehyde on the working electrode has a linear relation with the concentration of ethyl alcohol. An enzyme catalyst layer of the ethyl alcohol oxidase film is not conductive and is an electrochemical inactive layer, and all reactions relevant to enzyme have no direct relation with the current of the working electrode of the electrochemical sensor. Catalytic reaction products of enzyme are diffused into the working electrode for further reaction, and then electrochemical current is generated and has a linear relation with the concentration of ethyl alcohol. The working electrode of the sensor is a low-activity material. The response time of the ethyl alcohol sensor is short, test results are accurate, the ethyl alcohol sensor makes no obvious response to other organic gas but has extremely high selectivity and sensitivity to ethyl alcohol, and the ethyl alcohol sensor can better meet the requirement for ethyl alcohol detection in complicated environments.

Description

technical field [0001] The invention relates to the field of alcohol detection, which uses an electrochemical ethanol sensor using a biological enzyme catalytic preparation, in particular to an electrochemical ethanol sensor based on alcohol oxidase and a preparation method thereof. Background technique [0002] Blood alcohol concentration detection is now not only used as a law enforcement method to control and punish drunk driving, but also in positions that require a high degree of mental concentration, such as: crane drivers, outposts, train drivers, aircraft pilots, coal miners, steel plant furnace operators, doctors, etc. Etc., alcohol detection is also widely used. The most concise blood alcohol concentration test is the breath alcohol concentration test. The detection of breath concentration is closely related to blood alcohol concentration. [0003] At present, there are three schemes of alcohol testing sensors popular on the market: semiconductor alcohol sensor s...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/30
Inventor 朱良胜李琳刘刚
Owner SHENZHEN METERAGE SCI & TECH DEV CO LTD
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