Method for inducing garlic test tube bulb

A test tube and bulb technology is applied to a test tube bulb medium for inducing garlic and the field of rapid propagation thereof, and can solve the problems of competition for ground garlic nutrition, reduction of reproduction efficiency, nutritional deficiency and the like

Inactive Publication Date: 2012-11-28
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More reports are that garlic is propagated by using asparagus as seeds, because only mature asparagus can be used as seeds, and immature or young asparagus (to be exact, aerial scale buds) as seeds, because of their lack of nutrition, Reduced germination rate reduces reproductive efficiency
At the same time, in order to obtain more mature garlic that can be used as seeds, people have to allow the garlic sprouts of the left garlic to grow fully. In this way, its growth will inevitably compete for the nutrition of the garlic, affect the yield of the garlic, and reduce the seed production. Garlic Benefits

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  • Method for inducing garlic test tube bulb
  • Method for inducing garlic test tube bulb

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Garlic rapid propagation method:

[0051] (1) Harvesting and processing of garlic buds

[0052] 10 days after the garlic planted in the field is braided, pick 200 flower buds, each with a 20cm pseudostem, put them in the sun to dry, divide the flower buds into 2 parts, wrap the flower buds with pseudostems with sulfuric acid paper, and leave the coat open mouth plastic storage bag;

[0053] (2) Low temperature induction of aerial scale buds

[0054] Induce the flower buds for 30 days in a refrigerator at 4°C at low temperature, and remove the flower bud coat and pseudostem before rejuvenation;

[0055] (3) Rejuvenation of aerial scale buds

[0056] A. Take MS medium as the basic medium, add 0.5mg of a-naphthaleneacetic acid, 2.5mg of kinetin, 30g of sucrose, and 7.5g of agar to 1L of the basic medium, and set the volume to 1000ml. , 1.1kg / cm high temperature and high pressure sterilization for 20min, when the sterilized medium is cooled to 60°C, divide it into 100ml...

Embodiment 2

[0061] (1) Harvesting and processing of garlic buds

[0062] Seven days after the garlic planted in the field is braided, pick 200 flower buds, each with a 15-20cm pseudostem, put them in the sun to dry, divide the flower buds into 2 parts, and wrap the flower buds with pseudostems in sulfuric acid paper. An open plastic storage bag for the jacket;

[0063] (2) Low temperature induction of aerial scale buds

[0064] Induce the young garlic buds at a low temperature of 6°C for 30 days, and remove the outer coat and pseudostem after the aerial scale buds grow;

[0065] (3) Rejuvenation of aerial scale buds

[0066] A. Take MS medium as the basic medium, add 0.5mg of a-naphthaleneacetic acid, 2.5mg of kinetin, 30g of sucrose, and 7.5g of agar to 1L of medium, make the volume to 1000ml, adjust the pH value to 5.8, and store at 121°C , 1.1kg / cm high temperature and high pressure sterilization for 20min, when the sterilized culture medium is cooled to 60°C, divide it into 100ml c...

Embodiment 3

[0071] Adopt the comparative test result of garlic rapid propagation method of the present invention and conventional method as follows:

[0072] Comparative Test:

[0073]

[0074] Conclusion: Compared with the conventional method, the present invention uses 4 ℃ low temperature treatment of young garlic buds to induce aerial scale buds, rejuvenates the aerial scale buds on MS+2.5mg KT+0.5mg NAA medium, and rejuvenates the aerial scale buds on MS+2.5mg KT+0.5mg NAA medium. +60g / L sucrose medium induces test tube bulbs, improves the reproduction efficiency of garlic rapid propagation using aerial bulbs as seeds, reduces the cost of hormone use, and reduces the risk of genetic mutations. The invention provides an important possibility for rapid propagation and industrialized seedling cultivation of garlic.

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Abstract

The invention discloses a new method for inducing a garlic test tube bulb. The method mainly comprises the following steps of: performing low-temperature treatment of the garlic tender bud at 4 DEG C to induce atmogenic bulbil; rejuvenating atmogenic bulbil on an MS+2.5mg KT+0.5mg NAA culture medium; and inducing the test tube bulb on an MS+60g/L sucrose culture medium without hormone. The method can be used for improving the propagation efficiency of garlic rapid propagation by using atmogenic bulbil as seeds, and reducing the cost. By virtue of the method disclosed by the invention, the atmogenic bulbil induction rate can reach 100% (each bud can induce out atmogenic bulbil), each bud can induce out 25 atmogenic bulbils, all of the atmogenic bulbils can be induced into test tube bulbs, and the test tube bulb induction rate reaches 100%. Without cell dedifferentiation and redifferentiation in the whole induction process, the genetic mutation probability is reduced; and the method disclosed by the invention provides important possibility for the industrialized seedling production of garlic rapid propagation.

Description

technical field [0001] The invention belongs to the technical field of agricultural bioengineering, and relates to a method for garlic tissue culture and rapid propagation, in particular to a test-tube bulb culture medium for inducing garlic and a method for rapid propagation thereof. Background technique [0002] garlic( Allium sativum ) is an asexually propagated plant. Due to long-term asexual reproduction, garlic has accumulated a large number of viruses, which seriously affects the quality, yield and commerciality of garlic. Israeli scientists have promoted garlic flowering through low temperature induction, photoperiod regulation and nutrient control, obtained garlic seeds, and made gratifying progress through seed propagation and improved garlic varieties. However, due to the small biomass of garlic seeds, the number of seedlings obtained is limited. The requirements for obtaining seeds are high, so the propagation of garlic through garlic flowering inoculum is still...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王振英李转春彭爱芳刘晓颖范宝莉陈宏
Owner TIANJIN NORMAL UNIVERSITY
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