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Method for screening cold-resistant variety of steeplebush

A screening method, the technology of Spiraea, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of no screening and evaluation of plant cold resistance, no cold identification and screening of Spiraea resources, etc., to achieve The effect of improving cold resistance

Inactive Publication Date: 2012-04-18
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no method for screening and evaluating plant cold resistance that combines the semi-lethal temperature and cold resistance membership function, and there is no cold resistance identification and screening for abundant Spiraea resources.

Method used

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  • Method for screening cold-resistant variety of steeplebush
  • Method for screening cold-resistant variety of steeplebush
  • Method for screening cold-resistant variety of steeplebush

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Experimental program
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specific Embodiment approach 1

[0010] Specific embodiment one: the screening method of cold-resistant species of spiraea in the present embodiment is carried out according to the following steps: 1. Natural low temperature treatment above zero in the open field of spirea: the natural day and night average temperature in the open field in autumn reaches 15-5 ℃, which will be tested The pot seedlings of Spiraea chinensis were placed in the open field for 3 weeks, and then the leaves were taken when the natural day and night average temperature reached 15°C, 10°C and 5°C in the open field, and the relevant physiological indicators of cold resistance were measured, and then each pot seedling of Spiraea Continue to grow naturally in the open field with a temperature above zero; the pot seedlings of Spiraea chinensis are placed in the open field during the treatment period, and the natural day and night average light in the open field is 860-1100 μmol·m -2 ·s -1 , 200 plants of each kind of spirea pot seedlings a...

specific Embodiment approach 2

[0013] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the height of the Spiraea pot seedling described in step 1 and step 2 is 15-20 cm, the number of branches is 6-8, and the number of leaves is 20 ~30 pieces. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0014] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the cold-resistant physiological index described in step one, step two and step three is the soluble sugar content, the proline content, the soluble protein content of test branches or leaves Content, SOD enzyme activity, ABA content and membrane fatty acid content and photosynthetic physiological indicators. Others are the same as in the first embodiment.

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Abstract

The invention discloses a method for screening the cold-resistant variety of steeplebush and relates to a method for screening the cold-resistant variety of a garden tree, namely the steeplebush. The invention provides the method for screening the cold-resistant variety of the steeplebush, a technical system and the method for screening the cold-resistant variety of the steeplebush are established in advance for the first time, and the technical blank about the cold-resistance screening of the steeplebush at home and abroad is filled. The method comprises the following steps of: 1, performingoutdoor natural low-temperature treatment on the steeplebush; 2, performing persistent constant and low-temperature treatment on the steeplebush; 3, performing outdoor natural freezing treatment on the steeplebush; 4, calculating subordinate function values of cold-resistance physiological indexes; 5, performing simulative outdoor low-temperature gradient freezing treatment on the steeplebush, and calculating the semilethal temperature of the steeplebush; and 6, determining the cold resistance of the steeplebush according to the average subordinate function value and the semilethal temperature of the steeplebush so as to finish the screening of the cold-resistant variety. In the method, double results, namely the subordinate function values and the semilethal temperature of the cold resistance are combined, so that the simplicity of the cold-resistance screening is overcome, and the cold-resistance screening is highly consistent with the overwintering performance of the steeplebush. The invention provides technical support for screening cold-resistant steeplebush in northern gardens and is applied to the field of the cold-resistance screening of the steeplebush.

Description

technical field [0001] The invention relates to a screening method for cold-resistant species of spirea. Background technique [0002] Spiraea (Spiraea L.) is the original genus of the Rosaceae. It is rich in resources. There are more than 70 species distributed in my country. Spiraea plants have a long-lasting flowering period, pure and bright colors, and various leaf shapes. The leaves are all over the body, the garden ornamental value is high, and it has an ideal garden application prospect. There is a lack of landscaping resources in northern my country, monotonous greening tree species, and insufficient garden plant landscape. Spiraea is a good greening material. However, due to the cold climate in the north, it is not suitable for the growth of all kinds of spirea. Therefore, the research on the cold resistance ability of spirea resources and the selection of cold-resistant resources are the key to its scientific application in alpine regions. [0003] Severe cold is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/00
Inventor 刘慧民王大庆车代弟马艳丽房莉
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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