Method for extracting DHA from cells of algae and fungi by breaking cell walls

A technology for fungal cells and algae, applied in the field of extracting effective components, can solve the problems of organic residues affecting the final product, unsuitable for large-scale production and use, low efficiency, etc., to reduce the possibility of oxidative decomposition, overcome residues and explosions Hazardous, avoid decomposing or inactivating effects

Active Publication Date: 2010-09-01
XIAMEN HUISON BIOTECH
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AI Technical Summary

Problems solved by technology

However, this kind of traditional method has a relatively low wall breaking rate, is easy to cause environmental pollution, and is easy to destroy the required extraction products in the cells. Although organic solvent extraction is used in the extraction, although the extraction rate is relatively high and can reach 95%, it will also affect the final product. of organic residues
Another method is to use protease to break the wall, but it is often necessary to mix a variety of different proteases according to the situation to achieve the effect of breaking the wall. At the same time, the cost of adjusting the enzymatic hydrolysis conditions is relatively high, the efficiency is not high, and it is not suitable for large-scale production use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Add the Schizochytrium fermentation broth after fermentation to the storage tank of the horizontal spiral separation system through the pressure difference, and use the horizontal spiral separation system to separate and collect the Schizochytrium cells to keep the water content of the sludge below 80 %; Then the bacteria slime is collected in a storage tank with cooling coils and agitation, in order to keep the feed liquid with good fluidity and remove the soluble substances around the cells, use hydrochloric acid to adjust the pH of the bacteria sludge to 3.0, for further Subsequent treatment improves the wall breaking rate, and the adjusted bacteria sludge is stirred at 200rpm for 30min;

[0019] 2) For the bacteria sludge whose pH value has been adjusted in the storage tank, it is cooled by feeding frozen water with a temperature of 10°C into the coil of the tank, and when the temperature of the bacteria sludge drops to 15°C, close the inlet and outlet of the froz...

Embodiment 2

[0024] 1) Add the dinoflagellate fermentation liquid after the fermentation to the storage tank of the horizontal spiral separation system through the pressure difference, and use the horizontal spiral separation system to separate and collect the dinoflagellate cells and keep the water content of the sludge at 70% Then the bacteria slime is collected in a storage tank with cooling coil and stirring, in order to keep the feed liquid with good fluidity and remove the soluble matter around the cell, utilize citric acid to regulate the bacteria sludge pH2.0, for further Subsequent treatment improves the wall breaking rate, and the adjusted bacteria sludge is stirred at 250rpm for 20min;

[0025] 2) For the bacteria sludge whose pH value has been adjusted in the storage tank, it is cooled by feeding frozen water with a temperature of 10°C into the coil of the tank, and when the temperature of the bacteria sludge drops to 20°C, close the inlet and outlet of the frozen water;

[002...

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PUM

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Abstract

The invention provides a method for extracting DHA from cells of algae and fungi by breaking cell walls and relates to a method for extracting active ingredients from microorganisms. The method provided by the invention avoids the assistance from any organic solvent and chemical medicaments, breaks the walls of the cells of the algae and the fungi by using a physical method and extracts a grease product DHA (docosahexaenoic acid) from the cells and is suitable for large-scale production at low cost. The method comprises: after fermentation is finished, introducing fermentation liquor of microalgae or fungi to a separation system for separating and collecting the cells, adjusting the pH value of bacterial sludge with an acid to 2.0 to 4.0, keeping the temperature of the bacterial sludge between 10 and 20 DEG C, adding antioxidant into the bacterial sludge, and performing high-pressure homogenization and wall breaking by using a high-pressure homogenizer; and adding water into the bacterial sludge subjected to wall breaking, stirring the bacterial sludge, and separating feed liquor in a three-phase separator to obtain DHA grease. In the invention, physical wall breaking and the physical extracting method are used, the process is simple, the cell breaking is high efficient, the low-temperature treatment and antioxidant treatment of the bacterial sludge can effectively protect thebioactivities of the matters in the cells of the algae and the fungi, and the product is green, nontoxic and free from residues.

Description

technical field [0001] The invention relates to a method for extracting effective components from microorganisms, especially a method for extracting DHA (docosahexaenoic acid) from algae and fungal cells. Background technique [0002] Algae and fungi are an important part of the biosphere, and are the only bait for the entire growth process (or a specific developmental stage) of aquatic animals in the aquatic ecosystem or the bait for bait organisms. At the same time, microalgae and fungi are close to plant species. There are diversity, complexity and particularity in terms of biochemical composition, metabolic pathways, etc., so this determines its value in food, health care and medicine. Humans develop a large number of high-value-added biologically active products that humans need by cultivating a large number of microalgae and fungi. Therefore, microalgae and fungi play a huge role in the modern fermentation industry. Many antibiotics, vitamins, aminoheterocycles, polype...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C57/03C07C51/42
Inventor 钟惠昌
Owner XIAMEN HUISON BIOTECH
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