A bioactive peptide for promoting growth of piglets and its preparation method and application
A technology of bioactive peptides and piglets, applied in biochemical equipment and methods, methods based on microorganisms, applications, etc., can solve problems such as imperfect physiological functions of digestive glands, imperfect thermoregulatory functions, and poor resistance to cold stress , to achieve the effect of overcoming hormone residues, promoting growth and development, and increasing growth rate
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[0015] The third aspect of the present invention provides a method for preparing the above-mentioned bioactive peptide for promoting growth of piglets, comprising the following steps:
[0016] A. Culture after screening Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis respectively;
[0017] B. Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis obtained in step A are cultured and fermented, the supernatant of the fermentation broth is extracted, concentrated, dried, separated and purified to obtain bioactive peptides.
[0018] The biologically active peptides for promoting the growth and development of piglets are produced under the synergy of various enzyme-producing probiotics. Improve the enzyme production characteristics, enzyme production and enzyme activity of probiotics through artificial mutagenesis, and carry out synchronous compound culture of Enterococcus l...
Embodiment 1
[0033] All bacterial strains adopted in this embodiment are microbial strains that can be added in feed, and Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis are produced by the Agricultural Microbiology National Provided by key laboratories, Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis purchased on the market can also achieve very similar effects.
[0034] One, the preparation method of the bioactive peptide that promotes the growth of piglets of the present embodiment comprises the following steps:
[0035] Step 1: Cultivate strains, and optimize each strain by using mutation breeding technology.
[0036] Under an ultraviolet lamp with a power of 20W and an irradiation distance of 30cm, 2mL of the preserved bacterial suspensions of Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis were separated from the casein ...
Embodiment 2
[0057] All bacterial strains adopted in this embodiment are microbial strains that can be added in feed, and Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis are all purchased in the market , strains from different manufacturers can achieve similar results.
[0058] One, the preparation method of the bioactive peptide that promotes the growth of piglets of the present embodiment comprises the following steps:
[0059] Step 1: Cultivate strains, and optimize each strain by mutation breeding technology.
[0060] Under an ultraviolet lamp with a power of 20W and an irradiation distance of 30cm, 2mL of the preserved bacterial suspensions of Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis were separated from the casein separation and screening medium. On a sterile plate, irradiate for 2-3 minutes to culture and count, observe the transparent circle on the casein separation an...
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