A bioactive peptide for promoting growth of piglets and its preparation method and application

A technology of bioactive peptides and piglets, applied in biochemical equipment and methods, methods based on microorganisms, applications, etc., can solve problems such as imperfect physiological functions of digestive glands, imperfect thermoregulatory functions, and poor resistance to cold stress , to achieve the effect of overcoming hormone residues, promoting growth and development, and increasing growth rate

Active Publication Date: 2019-08-30
湖北博大生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The growth and development of piglets is a key link related to pig production. Due to the unique digestive physiological structure of piglets, their digestive organs are not perfect, the physiological functions of digestive glands are not perfect, they lack congenital immunity, and their body temperature regulation function is not perfect. Stress resistance is poor, and piglets have a high incidence of various diseases and low survival rates under normal feeding conditions

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  • A bioactive peptide for promoting growth of piglets and its preparation method and application

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preparation example Construction

[0015] The third aspect of the present invention provides a method for preparing the above-mentioned bioactive peptide for promoting growth of piglets, comprising the following steps:

[0016] A. Culture after screening Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis respectively;

[0017] B. Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis obtained in step A are cultured and fermented, the supernatant of the fermentation broth is extracted, concentrated, dried, separated and purified to obtain bioactive peptides.

[0018] The biologically active peptides for promoting the growth and development of piglets are produced under the synergy of various enzyme-producing probiotics. Improve the enzyme production characteristics, enzyme production and enzyme activity of probiotics through artificial mutagenesis, and carry out synchronous compound culture of Enterococcus l...

Embodiment 1

[0033] All bacterial strains adopted in this embodiment are microbial strains that can be added in feed, and Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis are produced by the Agricultural Microbiology National Provided by key laboratories, Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis purchased on the market can also achieve very similar effects.

[0034] One, the preparation method of the bioactive peptide that promotes the growth of piglets of the present embodiment comprises the following steps:

[0035] Step 1: Cultivate strains, and optimize each strain by using mutation breeding technology.

[0036] Under an ultraviolet lamp with a power of 20W and an irradiation distance of 30cm, 2mL of the preserved bacterial suspensions of Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis were separated from the casein ...

Embodiment 2

[0057] All bacterial strains adopted in this embodiment are microbial strains that can be added in feed, and Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis are all purchased in the market , strains from different manufacturers can achieve similar results.

[0058] One, the preparation method of the bioactive peptide that promotes the growth of piglets of the present embodiment comprises the following steps:

[0059] Step 1: Cultivate strains, and optimize each strain by mutation breeding technology.

[0060] Under an ultraviolet lamp with a power of 20W and an irradiation distance of 30cm, 2mL of the preserved bacterial suspensions of Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis were separated from the casein separation and screening medium. On a sterile plate, irradiate for 2-3 minutes to culture and count, observe the transparent circle on the casein separation an...

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Abstract

The invention provides a bioactive peptide for promoting the growth of piglets. The bioactive peptide contains the amino acid sequence shown in SEQ ID No. 1. The bioactive peptide can significantly improve the absorption and utilization rate of nutrients in feed by piglets, and promote the growth of piglets. Growth and development, reduce morbidity and improve piglet survival rate. Further, a preparation method of a bioactive peptide that promotes piglet growth and its application in promoting piglet growth and reducing piglet mortality is provided, and the bioactive peptide that promotes piglet growth and development is produced through the synergistic action of various enzyme-producing probiotics , used in piglets to promote the body's anabolism and fat decomposition, promote protein synthesis, achieve the purpose of promoting the growth of piglets and increase their growth speed, by regulating the hormone level of the endocrine system, play a role in promoting the growth and development of piglets, and overcome the hormone residues problems, to achieve the purpose of healthy and green farming.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a biologically active peptide that can effectively promote the growth and development of piglets, increase their growth speed and promote the growth of piglets, and its preparation method and application. Background technique [0002] The growth and development of piglets is a key link related to pig production. Due to the unique digestive physiological structure of piglets, their digestive organs are not perfect, the physiological functions of digestive glands are not perfect, they lack congenital immunity, and their body temperature regulation function is not perfect. Stress resistance is poor, and piglets have a high incidence of various diseases and a low survival rate under normal feeding conditions. Improving the absorption and utilization rate of nutrients in the feed by piglets, promoting the growth and development of piglets, reducing the inci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C12P39/00C12P21/02A23K20/147A23K50/30C12R1/46C12R1/10C12R1/07C12R1/72
Inventor 孙国平吴正荣范博文陈义杰李亮
Owner 湖北博大生物股份有限公司
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