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Skate liver oil and preparation method thereof

A technology for cod liver oil and cod liver, which is applied in the fields of edible oil/fat, food science, and application, can solve the problems of reduced extraction rate, a large amount of salt in by-products, and low demulsification effect, and achieves enhanced health care, low cost, The effect of improving stability

Active Publication Date: 2014-02-12
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, it is easy to form a stable emulsion system during the enzymatic extraction of cod liver oil, but most reports only obtain the crude cod liver oil in the upper layer by centrifugation, and discard the emulsion in the middle layer, resulting in a decrease in the extraction rate
It is also reported that multiple salting out methods are used for demulsification, but this method has the disadvantages of low demulsification effect and a large amount of salt in by-products.

Method used

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  • Skate liver oil and preparation method thereof

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preparation example Construction

[0028] The preparation and production steps of the present invention are as follows:

[0029] 1) Storage of cod liver: the ray liver, a by-product of ray processing, is cleaned and stored in a freezer below -18°C.

[0030] 2) Pretreatment: thaw the cod liver and chop it; it can also be frozen and thawed and then chopped. The freezing temperature is below -18°C.

[0031] 3) Enzymatic hydrolysis: add a certain amount of distilled water to the hydrolysis pot to make the liquid-solid ratio (mass) 1.5:1, add hydrochloric acid to adjust the pH to 8, add cod liver 2% (mass) compound protease (composed of papain and Alkaline protease, the mass ratio is 2:3), sealed, placed in a constant temperature shaker (130r / min, 45°C), and enzymatically hydrolyzed for 3.5h.

[0032] 4) Enzyme inactivation: After enzymatic hydrolysis, add hydrochloric acid to adjust the pH to 3.5 to denature and inactivate the complex protease.

[0033]5) Centrifugal separation: Pour out the enzymatic hydrolysis ...

Embodiment 1

[0048] After thawing 5kg of ray liver (crude fat content: 1.45kg), put it in a freezer below -18°C to freeze again, then thaw and chop it, put it in a hydrolysis pot, add 5L of distilled water, and add hydrochloric acid to adjust the pH to 7.5 , add 75g of complex protease (composed of 18.75g of papain and 56.25g of alkaline protease), seal it, place it in a constant temperature oscillator, and enzymolyze it at a speed of 100r / min and a temperature of 40°C for 3h. After enzymatic hydrolysis, add hydrochloric acid to adjust the pH to 3 to denature and inactivate the compound protease, pour out the enzymatic hydrolysis solution, rinse the residue at the bottom of the hydrolysis pot with an appropriate amount of hot water for 2 times, incorporate the solution into it, and then place it in a low-speed large-capacity centrifuge , centrifuged while hot at a rotating speed of 4000r / min for 5min, and separated to obtain 1201g of crude cod liver oil in the upper layer. The middle layer...

Embodiment 2

[0050] After thawing 5kg of ray liver (crude fat content: 1.45kg), put it in a freezer below -18°C to freeze again, then thaw and chop it, put it in a hydrolysis pot, add 10L of distilled water, and add hydrochloric acid to adjust the pH to 8.5 , add 125g of complex protease (composed of 62.5g papain and 62.5g alkaline protease), seal it, place it in a constant temperature oscillator, and enzymolyze it at a speed of 150r / min and a temperature of 50°C for 4h. After enzymatic hydrolysis, add hydrochloric acid to adjust the pH to 3.5 to denature and inactivate the compound protease, pour out the enzymatic hydrolysis solution, rinse the residue at the bottom of the hydrolysis pot with an appropriate amount of hot water for 3 times, incorporate the solution into it, and then place it in a low-speed large-capacity centrifuge , centrifuged while hot at a rotating speed of 5000r / min for 15min, and separated to obtain 1243g of upper layer crude cod liver oil. The middle layer lipoprote...

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Abstract

The invention relates to a skate liver oil and a preparation method thereof. The skate liver oil is characterized in that the skate liver oil comprises 0.76-1.13mass% of alkoxy glycerin, 0.71-0.92mass% of squalene, 25-28mass% of EPA and DHA, 1200-1600IU / g of vitamin A, 120-150IU / g of vitamin D, and the other substances which are lipids. The preparation method comprises the following steps: freezing skate livers, unfreezing, chopping, carrying out enzymatic hydrolysis treatment, carrying out enzyme killing treatment after the enzymatic hydrolysis, centrifuging, freezing the obtained emulsion, unfreezing, demulsifying, centrifuging, refining, degumming by citric acid, deacidifying by a caustic soda-soda ash mixture (a mass ratio of caustic soda to soda ash is 2:1), carrying out low temperature treatment, and carrying out evacuating nitrogen-filling packaging. The method has the advantages of reasonable operation, low cost and mild conditions, and the obtained skate liver oil has a very low peroxide value, a very low acid value and no nutrition loss, is rich in vitamin A, vitamin D, EPA, DHA, and important squalene and alkoxy glycerin, has a reinforced healthcare effect, and is easy to preserve and circuit in the market because of the evacuating nitrogen-filling packaging.

Description

technical field [0001] The invention relates to a cod liver oil and its production field, in particular to cod liver oil rich in squalene and alkoxyglycerin produced from the liver of rays. Background technique [0002] As a health-care oil, cod liver oil contains a variety of active ingredients such as vitamins A, D, E, EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid). Vitamins A, D, and E play an important role in the body's metabolism, growth, development, and health. They can promote bone health, improve vision sensitivity, and prevent chondrosis and night blindness. EPA and DHA are polyunsaturated fatty acids that are very important to the human body and are easily oxidized. The former can eliminate fatigue, lower blood pressure, fight cancer and prevent atherosclerosis and cerebral thrombosis, etc.; the latter can improve memory and brain function. In addition, some cod liver oils also contain important terpene compounds squalene and alkoxyglycerol compoun...

Claims

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Application Information

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IPC IPC(8): A23D9/02
Inventor 方旭波陈小娥余辉臧丽芹
Owner ZHEJIANG OCEAN UNIV
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