Cloning and function expression method of adversity stress AhMYBL6 gene in peanut

Abstract

The invention relates to a cloning and function expression method of an adversity stress AhMYBL6 gene in peanut. The adversity stress AhMYBL6 gene is synthesized by RT-PCR (reverse transcription-polymerase chain reaction) cloning by the steps of preparation and treatment of materials, extraction of RNA (ribonucleic acid) and synthesis of cDNA (complementary deoxyribonucleic acid). The open reading frame of the gene is 861bp, and 287 amino acids are coded totally. The homology of the amino acid sequence of the gene with the MYb family proteins GmMYB4, GmMYB29 and GmMYB29A2 of the soybean is higher than 60%. The fluorescent quantitative PCR verifies the expression patterns of the AhMYBL6 under low temperature, salt stress and drought stress; the result indicates that the expression levels of the AhMYBL6 are obviously enhanced after various stress treatment for 1 hour, and kept at high levels all the time thereafter; and the expression level of the AhMYBL6 in the leaf subjected to low-temperature treatment is maximally enhanced by 20 times. After the gene is transferred into peanut by transgenic means, compared with the control, the transgenic plant has obvious characteristics of cold tolerance, salt tolerance and drought tolerance. The AhMYBL6 gene can obviously enhance the stress tolerance of the peanut.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a peanut adversity stress AhMYBL6 Gene cloning and functional expression methods. Background technique [0002] Peanut is a leguminous arachis crop, rich in oil and protein, and is an important oil crop and economic crop in my country. The yield and quality of peanuts are seriously affected by drought, saline-alkali and other stresses, and the annual reduction rate of peanuts caused by drought in the whole country reaches more than 20%. However, due to the narrow genetic basis of peanut variety resources and the lack of high drought-resistant, cold-resistant, and salt-tolerant gene sources, it is difficult to breed high-resistant varieties using conventional breeding methods. With the rapid development of molecular biology, the use of transgenic technology to improve plant stress tolerance has achieved remarkable results in recent years, and a deeper understanding of stress-related ge...

AI Technical Summary

Problems solved by technology

A large number of studies have shown that during various abiotic stresses, the accumulation of MYB family proteins plays a potential protective role in plants under stress conditions, but there are no reports on the cloning and functional studies of this class of genes trapped in peanuts

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