Preparation method of liquid acidic pectinase

A technology of acid pectinase and liquid, which is applied in the field of preparation of liquid acid pectinase, to achieve the effects of increased specific surface area, strong inhibitory effect, and significant antibacterial properties

Inactive Publication Date: 2015-04-22
湖南新鸿鹰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the defects of the existing liquid acid pectinase, without adding chemical preservatives, without low-temperature treatment, and adopting high-voltage pulse electric field treatment to the fermented crude enzyme liquid and the deployment c...

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  • Preparation method of liquid acidic pectinase
  • Preparation method of liquid acidic pectinase

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1 raw material preparation

[0058] 1. Preparation of Pectin Decomposition

[0059]The preparation method of the decomposed pectin product is as follows: fresh hawthorn pomace, citrus peel, and apple pomace are respectively put into an ultrasonic cleaning machine equipped with 1.2% sodium bicarbonate solution, cleaned at 200W and 40KHz for 8min, drained, Cut into pieces or dices of equal size, length and width 5-10mm, thickness 3-5mm, mix evenly according to the mass ratio 6:3:2, then freeze at -23°C for 20min; crush immediately, the particle size is 0.5mm Add deionized water of 4 times the amount of pulverized matter, mix well, adjust the pH value to 2.5 with citric acid, and carry out high-voltage pulse electric field treatment under the condition of electric field strength 40kV / cm, pulse time 500μs, and pulse frequency 250Hz at room temperature, then Boil at normal pressure for 40 minutes, filter with a cloth bag, boil the filter residue with a filtrate t...

Embodiment 2

[0069] A preparation method of liquid acid pectinase, comprising the steps of:

[0070] 1) Add 200 mesh diatomite to the acid pectinase fermentation liquid obtained after fermentation, pre-coat diatomite with a 600 mesh filter cloth, filter with a plate and frame filter, and operate at a pressure of 0.18Mpa to obtain fermented crude enzyme liquid;

[0071] The addition amount of described diatomaceous earth is that every 100mL fermented liquid adds 1g;

[0072] 2) The fermented crude enzyme liquid in step 1) is subjected to high-voltage pulse electric field treatment under the conditions of electric field strength 25kV / cm, pulse time 300μs, and pulse frequency 250Hz at room temperature;

[0073] 3) Step 2) The fermented crude enzyme liquid treated by the high-voltage pulsed electric field is first passed through a microfiltration membrane with a pore size of 0.6 μm, the inlet pressure of the microfiltration is 0.11 MPa, the pressure difference is 0.08 MPa, and the temperature ...

Embodiment 3

[0082] A preparation method of liquid acid pectinase, comprising the steps of:

[0083] 1) Add 100 mesh diatomite to the acid pectinase fermentation liquid obtained after fermentation, pre-coat diatomite with a 500 mesh filter cloth, filter with a plate and frame filter, and operate at a pressure of 0.14Mpa to obtain fermented crude enzyme liquid;

[0084] The addition amount of described diatomaceous earth is every 100mL fermented liquid adds 0.5g;

[0085] 2) The fermented crude enzyme liquid in step 1) is subjected to high-voltage pulse electric field treatment under the conditions of electric field strength 20kV / cm, pulse time 200μs, and pulse frequency 200Hz at room temperature;

[0086] 3) Step 2) The fermented crude enzyme liquid treated by the high-voltage pulsed electric field is first passed through a microfiltration membrane with a pore size of 0.4 μm, the inlet pressure of the microfiltration is 0.1 MPa, the pressure difference is 0.07 MPa, and the temperature of t...

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Abstract

The invention discloses a preparation method of a liquid acidic pectinase, belonging to the technical field of enzyme preparations. The preparation method of the liquid acidic pectinase comprises the steps of treating a fermented crude enzyme solution and a blended concentrated solution at normal temperature by using a high-voltage pulsed electric field, and adding a pectin decomposer, a Chinese herb extract, modified dietary fibers and other natural plant raw materials in a blending process, wherein chemical preservatives do not need to be added, and low-temperature treatment is not needed; and then, degerming, filtering, and carrying out sterile filling to obtain the liquid acidic pectinase with stability and long shelf life. Experiments prove that the total bacterial count is 68-102CFU/mL if the liquid acidic pectinase prepared by using the preparation method is stored at room temperature for 12 months; and the enzyme activity loss ratios are respectively 1-1.4% and 0.5-1.1% if the liquid acidic pectinase is stored at 40 DEG C and 0 DEG C for 12 months. The preparation method is simple in process, convenient to operate, energy-saving and environment-friendly.

Description

technical field [0001] The invention relates to a preparation method of acid pectinase, in particular to a preparation method of liquid acid pectinase. Background technique [0002] Liquid enzyme preparation is a raw material that is easy to use, environmentally friendly and has good biodegradability. Specific catalytic properties and mild reaction conditions have been more and more used and valued in the industry. In terms of use, liquid enzyme preparations have the following advantages: 1) liquid enzyme preparations are dispersed in the substrate in the state of molecules or particles, with large dispersion, fast absorption, rapid enzymolysis reaction, and high enzyme activity; 2) easy 3) It is beneficial to improve the utilization rate of enzyme preparations, 4) It can be used directly without dissolving and activating; 5) It has high purity and low impurity content; 6) The production process is short, Low cost; 7) Dust pollution will not be caused during production, pr...

Claims

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Application Information

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IPC IPC(8): C12N9/88
CPCC12N9/2402C12Y302/01015
Inventor 李洪兵张锦杰李海清朱永明胡永明王小玲
Owner 湖南新鸿鹰生物工程有限公司
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