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Method for synthesizing valtrate by utilizing ceratobasidium sp.AG-A

A kind of technology of keratobasidium and valerianin, which is applied in the field of synthesizing valerin by using keratobasidium, can solve the problems such as the reduction of valerin yield, and achieve the effects of stable results, simple operation, and improved yield.

Inactive Publication Date: 2017-04-19
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production of valerianin in the existing method for synthesizing valerianin by the horn basidiomycetes is relatively reduced, and its production needs to be further improved to meet the needs of large-scale fermentation and production of valerianin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A kind of embodiment of the method that the present invention utilizes claria to synthesize valerenin, the method may further comprise the steps:

[0032] (1) Activation of Ceratobasidium species:

[0033]Pick the mycelium of the clast basidium from the refrigerated test tube slope and inoculate it on the plate of the solid medium, and culture it upside down at 26°C for 56 hours. On the base plate, maintain the temperature and continue to invert for 5 days to obtain mature mycelium. The components of the solid medium include: 200 g / L of peeled potatoes, 20 g / L of glucose, 20 g / L of agar, the pH of the solid medium is 6.5, and the solid medium is sterilized at 121° C. for 20 minutes before use. Among them, the Ceratobasidium sp. AG-A XCS-6 used is an endophytic fungus capable of producing valerenin, which has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. , the address of the depository institutio...

Embodiment 2

[0050] A kind of embodiment of the method that the present invention utilizes claria to synthesize valerenin, the method may further comprise the steps:

[0051] (1) Activation of Ceratobasidium species:

[0052] Pick the mycelium of the clast basidium from the refrigerated test tube slope and inoculate it on the plate of the solid medium, and culture it upside down at 28°C for 60 hours. On the base plate, maintain the temperature and continue to invert for 5 days to obtain mature mycelium. The components of the solid medium include: 200 g / L of peeled potatoes, 20 g / L of glucose, 18 g / L of agar, the pH of the solid medium is 6.2, and the solid medium is sterilized at 121° C. for 25 minutes before use. Among them, the Ceratobasidium sp. AG-A XCS-6 used is an endophytic fungus capable of producing valerenin, which has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. , the address of the depository instituti...

Embodiment 3

[0062] A kind of embodiment of the method that the present invention utilizes claria to synthesize valerenin, the method may further comprise the steps:

[0063] (1) Activation of Ceratobasidium species:

[0064] Pick the mycelium of the clastobasidium from the refrigerated test tube slope and inoculate it on the plate of the solid medium, and incubate it upside down at 27°C for 64 hours. On the base plate, keep the temperature and continue to invert for 4 days to obtain mature mycelium. The components of the solid medium include: 200 g / L of peeled potatoes, 20 g / L of glucose, 16 g / L of agar, the pH of the solid medium is 6.8, and the solid medium is sterilized at 121° C. for 30 minutes before use. Among them, the Ceratobasidium sp. AG-A XCS-6 used is an endophytic fungus capable of producing valerenin, which has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. , the address of the depository institution ...

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Abstract

The invention discloses a method for synthesizing valtrate by utilizing ceratobasidium sp.AG-A. The method comprises the following steps of (1) inoculating a solid culture medium with ceratobasidium sp.AG-A mycelia for inverse culture, and after mycelium germination, selecting contamination free fresh mycelia in the periphery and inoculating a fresh solid culture medium with the fresh mycelia for continuous inverse culture to obtain mature mycelia, wherein the used ceratobasidium sp.AG-A is a collected strain in the China general microbiological culture collection center (CGMCC) and has a collection number of CGMCC No.9817; (2) inoculating a seed culture medium with the mature mycelia obtained in the step (1), and performing shaking culture to obtain a seed solution; and (3) inoculating a fermentation culture medium with the seed solution obtained in the step (2) for performing fermentation culture, adding citronellal to the fermentation culture medium in batches in the fermentation culture process, and then continuing to perform shaking fermentation culture to obtain a valtrate-containing culture solution. According to the method, the yield of synthesizing the valtrate by the ceratobasidium sp.AG-A can be effectively increased.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for synthesizing valerenin by using Ceratobacterium. Background technique [0002] Valerian is an herbaceous plant belonging to the Patella family. It has a long history of medication in my country and is an important Chinese herbal medicine used blindly as a sedative. Valerian is used as medicine with massive roots and rhizomes, and has a wide range of pharmacological effects. Traditional Chinese medicine believes that valerian is flat and pungent in nature, and has the effects of relieving spasms, relieving pain, and calming the nerves. Modern medical research shows that valerian also has pharmacological effects such as anti-oxidation, anti-tumor, and anti-senile dementia. Valerian contains valerianin, valerian aldehyde, valerenyl terpene aldehyde, valerian alkaloid and other active ingredients. The latest research shows that valerian is the main component...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12R1/645
CPCC12P7/40
Inventor 黎继烈谭云方勤敏王卫蔡燕张梦君赵梦瑞谈泰猛任泽文祝久香莫俊恺
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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