Microreactor array chip for nucleic acid synthesis and preparation method thereof

A microreactor and array chip technology, applied in the field of synthetic biology, can solve the problems of low oligonucleotide synthesis concentration and redundant oligonucleotide synthesis steps, etc.

Pending Publication Date: 2022-05-13
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the single oligonucleotide synthesis concentration of the oligonucleotide synthesis chip is low, close to the femtomolar level, which cannot meet the needs of oligonucleotide assembly and artificial

Method used

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  • Microreactor array chip for nucleic acid synthesis and preparation method thereof
  • Microreactor array chip for nucleic acid synthesis and preparation method thereof
  • Microreactor array chip for nucleic acid synthesis and preparation method thereof

Examples

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Embodiment 1

[0051] Such as figure 1 As shown, the microreactor array chip for nucleic acid synthesis, from top to bottom, is the microreactor array 4 after nanoparticle aggregation and the synthesis substrate chip 1, wherein the microreactor array has good biocompatibility A permanent solid phase carrier (a solid support for nucleic acid synthesis) is used to provide the physical synthesis area required for nucleic acid synthesis. The material of the substrate chip is a high temperature or high pressure resistant material, which is used to provide a plane for the formation of microreactor arrays Support carrier and hydrophilic-hydrophobic interface;

[0052] The nanoparticle solid phase carrier required for the preparation of the microreactor array chip is a monodisperse silica microsphere (also can be a monodisperse polystyrene microsphere, silicon nanoparticle, iron ferric oxide nanoparticle, nano gold, nano-silver, nano-copper);

[0053] The particle diameter of the nanoparticle soli...

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Abstract

The invention provides a nucleic acid synthesis-oriented micro-reactor array chip and a preparation method thereof, the micro-reactor array chip with a high surface area is constructed by ink-jet printing of a nanoparticle droplet array on the surface of a synthetic chip and combination of a three-phase contact line regulation and control method and a high-temperature sintering technology at the same time, and the nucleic acid synthesis-oriented micro-reactor array chip is prepared. High-flux and high-load synthesis is realized, the overall synthesis yield of the synthetic chip is improved, and the method can be applied to the field of organic synthesis.

Description

technical field [0001] The invention relates to the field of synthetic biology, in particular to a nucleic acid synthesis-oriented microreactor array chip and a preparation method thereof. Background technique [0002] Synthetic biology uses the method of de novo synthesis to artificially synthesize genomes and artificially create new biological systems, which are disruptive innovative technologies that urgently need key breakthroughs in this century. Nucleic acid synthesis technology is a "stuck neck" technology in the development of synthetic biology, and the oligonucleotide synthesizer is its core equipment. Since the 1990s, the United States, Britain and other countries have carried out oligonucleotide synthesis on the basis of classical chemical methods. Instrument development. At present, column oligonucleotide synthesizers based on phosphoramidite chemical synthesis are widely used in the market. This column synthesizer achieves a yield of about micromoles per colum...

Claims

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Application Information

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IPC IPC(8): B01J19/00C12M1/00C12M1/40
CPCB01J19/0093C12M21/18C12M23/02
Inventor 栗大超张祎苏晓李小平
Owner TIANJIN UNIV
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