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High-yield mesoporous silica nano-particle and folic acid targeting modification method thereof

A mesoporous silica and nanoparticle technology, applied in the field of medicine, can solve the problems of uneven particle size and low silica yield, and achieve the effect of simple synthesis process and improved efficiency

Inactive Publication Date: 2016-08-31
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strong alkalinity of sodium hydroxide in this method, the yield of the prepared silicon dioxide is low, generally around 10-100 mg, and the particle size is not uniform.

Method used

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  • High-yield mesoporous silica nano-particle and folic acid targeting modification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of synthetic method that utilizes sol-gel method to prepare high-yield silica nanoparticles, the concrete steps are as follows:

[0029] 1) Take a clean single-neck bottle, and prepare a solution of cetyltrimethylammonium bromide (CTAB) with a final concentration of 0.5% in it. 5 ml of absolute ethanol and 50 μl of ethylene glycol amine were added, heated at 80° C., and the reaction was stirred for 10 minutes under a magnetic force of 100 rpm. Measure 0.1 ml of ethyl orthosilicate in the single-necked bottle and heat at 80°C, under 100 rev / min magnetic conditions, stir and react for 10 minutes, then turn off the heating and then under 100 rev / min magnetic conditions, stir and react for 120 minutes. Immediately after the reaction was completed, centrifuge at 9,000 rpm for 5 minutes, and washed and precipitated with absolute ethanol once to obtain high-yield silica nanoparticles, which were stored after being vacuum-dried.

[0030] 2) Method for Mesoporous Silica ...

Embodiment 2

[0032] A kind of synthetic method that utilizes sol-gel method to prepare high-yield silica nanoparticles, the concrete steps are as follows:

[0033] 1) Take a clean single-neck bottle, and prepare a solution of cetyltrimethylammonium bromide (CTAB) with a final concentration of 0.6% in it. 6 ml of absolute ethanol and 60 microliters of ethylene glycol amine were added, heated at 75°C, and the reaction was stirred for 12 minutes under a magnetic force of 150 rpm. Measure 0.15 milliliters of ethyl orthosilicate in the single-necked bottle and heat at 75°C, under 150 rev / min magnetic conditions, stir and react for 12 minutes, then turn off the heating and then under 150 rev / min magnetic conditions, stir and react for 100 minutes. Immediately after the reaction was completed, centrifuge at 7,500 rpm for 4 minutes, and washed and precipitated twice with absolute ethanol to obtain high-yield silica nanoparticles, which were stored after being vacuum-dried.

[0034] 2) Method of M...

Embodiment 3

[0036] A kind of synthetic method that utilizes sol-gel method to prepare high-yield silica nanoparticles, the concrete steps are as follows:

[0037]1) Take a clean one-mouth bottle, and prepare a solution of cetyltrimethylammonium bromide (CTAB) with a final concentration of 0.7% into it. Add 7 ml of absolute ethanol and 70 microliters of ethylene glycol amine, heat at 80° C., and stir for 15 minutes under magnetic conditions of 200 rpm. Measure 0.2 ml of tetraethyl orthosilicate into a one-necked bottle, heat at 80°C, stir and react for 15 minutes under 200 rpm magnetic condition, then turn off the heating and then stir and react for 90 minutes under 200 rpm magnetic condition. Immediately after the reaction, centrifuge at 7,000 rpm for 3 minutes, and wash the precipitate with absolute ethanol 3 times to obtain high-yield silica nanoparticles, which are dried in vacuum and stored.

[0038] 2) Method for mesoporous silica nanoparticles Dissolve the silica nanoparticles in 1...

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Abstract

The invention relates to a high-yield mesoporous silica nano-particle and a folic acid targeting modification method thereof. A preparation method for the high-yield mesoporous silica nano-particle comprises the following steps: 1) synthesis of a silica nano-particle: preparing the silica nano-particle with a diameter of 300 to 500 nm from ethyl orthosilicate by using a sol-gel method; and 2) preparation of a mesoporous silica nanometer carrier: removing a template of cetyl trimethyl ammonium bromide (CTAB) by using sodium chloride so as to prepare the mesoporous silica nanometer carrier with a pore size of 0.5 to 2 nm. Meanwhile, folic acid targeting modification of the mesoporous silica nano-particle is carried out. The invention has the beneficial effects that synthesis process is simple, nontoxic and fast; the prepared mesoporous silica nano-particle has pore size of 0.5 to 2 nm and synthesis output is as high as 500 to 1000 mg, substantially increased compared with the normal output of 10 to 100 mg in the prior art; and after folic acid targeting modification of the mesoporous silica nano-particle, the carrier has improved efficiency in entering tumor cells.

Description

technical field [0001] The invention belongs to the technical field of medicine; in particular, it relates to a high-yield mesoporous silica nanoparticle and a method for targeted modification of folic acid. Background technique [0002] Nano-drug carrier refers to a new type of carrier with a particle size of 10-1000 nanometers. It uses nanoparticles as carriers of drugs or dyes, and wraps drug therapeutic molecules or other functional molecules in nanoparticles or adsorbs them on them. surface. Nanocarriers have the following advantages: high drug loading and encapsulation efficiency, simple preparation and purification methods, low or no toxicity, appropriate particle size and particle shape, long circulation time in vivo, prolonging the circulation time of nanoparticles in vivo, The concentration of the contained active ingredient in the central chamber can be increased and the circulation time can be prolonged, so that the drug can better play the role of systemic trea...

Claims

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Application Information

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IPC IPC(8): C01B33/18A61K47/48A61K9/14
CPCC01B33/18C01P2004/62C01P2006/16
Inventor 常津郑斌王汉杰谌红彬潘慧卓陈德光
Owner TIANJIN UNIV
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