Engineering bacterium for high yielding of salidroside and/or tyrosol and application of engineering bacterium

Abstract

The invention relates to the field of bioengineering, in particular to an engineering bacterium for high yielding of salidroside and / or tyrosol and application of the engineering bacterium. The engineering bacterium for high yielding of the salidroside is recombinant yeast obtained by processing saccharomyces cerevisiae in such a way that a codon for coding an amino acid at a 229th site in an ARO4gene is mutated into a codon for coding leucine, a codon for coding glycine at a 141st site in an ARO7 gene is mutated into a codon for coding serine, and a codon for coding lysine at a 222nd site inan ARO3 gene is mutated into a codon for coding leucine. The invention further discloses fermentation for preparing the salidroside through the engineering bacterium. The engineering bacterium can produce the salidroside and / or the tyrosol at the high yield, and has high economic value and good industrial application prospects.

Description

[0001] This application claims the priority of the Chinese patent application submitted to the China Patent Office on September 25, 2018, the application number is 201811116170.0, and the invention title is "an engineering bacterium with high salidroside production and its application", the entire content of which is passed References are incorporated in this application. technical field [0002] The invention relates to the field of bioengineering, in particular to an engineering bacterium with high salidroside and / or tyrosol production and application thereof. Background technique [0003] Rhodiola is a rare wild plant that grows in the alpine and pollution-free areas. It is a traditional drug used by the Tibetan people in my country. It has a history of more than 1,000 years of application. It has the functions of stimulating the nervous system, increasing work efficiency, eliminating fatigue and preventing altitude sickness. , It also has the functions of protecting cardi...

AI Technical Summary

Problems solved by technology

[0013] Although the production of salidroside in the subsequent recombinant large intestine was increased to a concentration of 0.7g / L, the low concentration undoubtedly increased the difficulty and cost of later extraction; two kinds of large intestine with glucose and xylose as substrates Bacillus co-cultivation technology, although 6.03g / L salidroside can be obtained, using more expensive xylose as substrate, also increases the production cost; although by optimizing the synthetic pathway of Saccharomyces cerevisiae, it will use cheap glucose as substrate Synthetic salidroside production increased to 1-1.1g / L, but for industrial production, there is still a demand for optimization and improvement

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