2 results about "Xylulose kinase" patented technology

Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding a xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganism comprises at least one nucleic acid sequence encoding a xylulokinase and / or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganism is capable of growing on xylose as a carbon source.

The invention belongs to the technical field of microbial genetic engineering and industrial fermentation, and discloses an aspergillus niger engineering strain for producing L-malic acid by using xylose and a construction method and application of the aspergillus niger engineering strain. A three-gene recombination strain of an XR gene for coding xylose reductase, an XDH gene for coding xylitol dehydrogenase and an XK gene for coding xylulokinase through simultaneous overexpression is obtained through directional construction. Through synergistic overexpression of the XR gene, the XDH gene and the XK gene, the limitation that the starting strain LH-1 cannot utilize the xylose is successfully broken through, and effective utilization and directional conversion of the xylose are realized. The strain can be used for producing L-malic acid by using a low-cost xylose raw material, so that the production cost is remarkably reduced, a new technical path is provided for industrial production of organic acid, and the strain has a wide industrial application prospect.