8 results about "Xylulose kinase" patented technology

The invention discloses recombinant Escherichia coli for producing xylitol by efficiently utilizing L-arabinose without adding glycerol and application, and belongs to the technical field of genetic engineering. Escherichia coli with an araBAD operon and an L-xylulokinase gene lyxK knocked out is used as a starting strain, a glucose specific transporter gene ptsG and a D-xylulokinase gene xylB in a genome are knocked out, and a global regulatory factor CRP is mutated; on the basis of an araA, dpe and lxr three-enzyme expression universal method reported at present, the gene arrangement sequence and copy number in three-enzyme tandem expression are optimized, a promoter and an RBS sequence are optimized and screened, and three-enzyme genes are integrated to a specific position of an escherichia coli chromosome in an optimized multi-copy form. According to the method, the inhibition effect of glucose on L-arabinose is achieved, the in-vivo multienzyme catalysis efficiency of existing synthesis of xylitol from arabinose is remarkably improved, and the yield of precursor arabitol and the yield of the product xylitol are greatly improved.

The invention relates to a method for characterizing intracellular NADPH (Nicotinamide Adenine Dinucleotide Phosphate) generation amount of yarrowia lipolytica through xylose consumption, and belongs to the technical field of biology. According to the method, the yarrowia lipolytica overexpressing xylose reductase, xylitol dehydrogenase and xylulokinase is inoculated into a culture medium taking xylose as a unique carbon source for culture, and a linear relation between the xylose consumption and the NADPH generation amount is established, so that the generation level of the NADPH can be represented by measuring the xylose consumption in the culture medium. The invention provides an effective method for characterizing the intracellular NADPH level of the yarrowia lipolytica, the intracellular NADPH level can be characterized only by detecting the consumption of xylose, and the detection method is simple and efficient.

The present invention belongs to the field of microbial technology, and relates to a genetically engineered bacterium for preparing ethanol based on hydrolyzed sugar from wood fiber and its application. The bacterium is a high xylose utilization and inhibitor tolerance engineered bacterium. The engineered bacterium is based on Saccharomyces cerevisiae S288C as a chassis strain, and co-expresses heterologous xylose reductase-xylitol dehydrogenase pathway-related genes, xylose transport-related genes, xylose isomerase-related genes and xylose isomerase genes, and over-expresses endogenous xylulose kinase-related genes, non-oxidative pentose phosphate pathway-related genes, reduced glutathione system genes, superoxide dismutase expression system-related genes, NADPH / NADP + The genes and transcription factors related to the cofactor circulation system were knocked out, the genes related to the competitive metabolic pathway were knocked out, and the bacteria were domesticated with sugar sources and inhibitors as pressure; the engineered bacteria can efficiently metabolize xylose and produce high-concentration ethanol in non-detoxified lignocellulose hydrolysate with high inhibitor concentrations and without any nutrient salt addition.

The invention provides a strain for producing beta-carotene as well as a construction method and application of the strain, and belongs to the technical field of synthetic biology and genetic engineering. The construction method of the MYy107 strain disclosed by the invention comprises the following steps: step 1, integrating a mevalonate kinase gene ERG12, a phosphomevalonate kinase gene ERG8, a mevalonate diphosphate decarboxylase gene ERG19 and an isopentene diphosphate isomerase gene IDI1 on an RT08 bacterium, so as to obtain an RT10 strain; 2, a xylose reductase gene XYL1, a xylitol dehydrogenase gene XYL2, a xylose isomerase mutant XylA3 * and a xylulokinase gene Yl.Xk are integrated on the RT10 strain, and the MYy107 strain is obtained. According to the MYy107 strain constructed by the invention, the beta-carotene can be produced by using xylose and acetic acid at the same time, and the yield of the beta-carotene is remarkably improved and reaches 710.01 mg / L.

Described herein are microorganisms, in particular yeast, which have been transformed with one or more expression construct(s) for i) the overexpression of the native genes encoding xylulose kinase (XKS1), transaldolase (TAL1), transketolase 1 (TKL1) and transketolase 2 (TKL2) and ii) the expression of a functional heterologous gene encoding a xylose isomerase (XI), where the xylose isomerase (XI) gene is derived from a microorganism selected from the group consisting of T. neapolitana, A. andensis and C. clariflavum. Also described herein are expression constructs, methods for fermenting pentose sugars using the microorganisms and methods for producing such microorganisms.

Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding a xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganism comprises at least one nucleic acid sequence encoding a xylulokinase and / or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganism is capable of growing on xylose as a carbon source.

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant foal foal xylose as well as a construction method and application thereof. The recombinant foal foal capable of improving the bacterial cellulose yield and the xylose utilization amount is constructed by taking the foal foal foal as an original strain and overexpressing a xylose isomerase gene xylA, a xylulokinase gene xylB, a phosphopentose pathway key gene TKL and a structural gene BcsD. Under the fermentation conditions of pure xylose and xylose and glucose, the bacterial cellulose yields of the bacterial strain can reach 9.51 g / L and 8.1 g / L respectively and are increased by 553.23% and 44.75% respectively compared with original bacteria, and the highest xylose consumption of the bacterial strain under the fermentation conditions of pure xylose can reach 11.18 g / L. According to the method, the utilization rate of xylose is remarkably increased, the production efficiency of bacterial cellulose is improved, and a feasible solution is provided for replacing efficient conversion of traditional biomass resources with glucose as a carbon source.

The invention belongs to the technical field of microbial genetic engineering and industrial fermentation, and discloses an aspergillus niger engineering strain for producing L-malic acid by using xylose and a construction method and application of the aspergillus niger engineering strain. A three-gene recombination strain of an XR gene for coding xylose reductase, an XDH gene for coding xylitol dehydrogenase and an XK gene for coding xylulokinase through simultaneous overexpression is obtained through directional construction. Through synergistic overexpression of the XR gene, the XDH gene and the XK gene, the limitation that the starting strain LH-1 cannot utilize the xylose is successfully broken through, and effective utilization and directional conversion of the xylose are realized. The strain can be used for producing L-malic acid by using a low-cost xylose raw material, so that the production cost is remarkably reduced, a new technical path is provided for industrial production of organic acid, and the strain has a wide industrial application prospect.