Method using ocean alpha-cyclodextrin glucosyltransferase to convert starch so as to prepare alpha-cyclodextrin

A glucose-based and cyclodextrin technology, applied in fermentation and other directions, can solve problems such as shortage, and achieve the effect of simple process, high purity and high conversion rate

Inactive Publication Date: 2017-03-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main problem of biological production of α-cyclodextrin is the lack of cyclodextrin glucosyltransferase that specifically converts starch to α-cyclodextrin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Potato starch and water were adjusted to a water slurry at a concentration of 15 wt%, and stirred for 10 minutes at 85°C for gelatinization; α-cyclodextrin glucosyltransferase with an enzyme activity of 200 units was added per gram of starch, and The volume is n-decyl alcohol with 5% starch mass, reacted for 6 hours, and stopped the reaction. The reaction product was filtered under airtight conditions, and the insoluble filter cake was washed repeatedly with distilled water to collect the filter cake. Add absolute ethanol with an initial reaction volume of 30%, stir the reaction at room temperature for 5 hours, and put it into a separatory funnel to stand still for 5 hours. Liquid separation and filtration, the supernatant liquid is a mixture of absolute ethanol and n-decyl alcohol, the lower liquid is α-cyclodextrin solution, and the lower liquid is evaporated in a rotary evaporator to obtain α-cyclodextrin. The total conversion rate of potato starch reaches 68.2%, an...

Embodiment 2

[0021] Adjust tapioca starch and water to a concentration of 15wt% to make a water slurry, and stir for 10 minutes at 85°C for gelatinization; add α-cyclodextrin glucosyltransferase with an enzyme activity of 200 units per gram of starch, add The volume is n-decyl alcohol with 5% starch mass, reacted for 6 hours, and stopped the reaction. The reaction product was filtered under airtight conditions, and the insoluble filter cake was washed repeatedly with distilled water to collect the filter cake. Add absolute ethanol with an initial reaction volume of 30%, stir the reaction at room temperature for 5 hours, and put it into a separatory funnel to stand still for 5 hours. Liquid separation and filtration, the supernatant liquid is a mixture of absolute ethanol and n-decyl alcohol, the lower liquid is α-cyclodextrin solution, and the lower liquid is evaporated by a rotary evaporator to obtain α-cyclodextrin. The total conversion rate of cassava starch reaches 68.5%, and the prop...

Embodiment 3

[0023] Adjust cornstarch and water to a concentration of 15wt% to make a water slurry, and stir at 85°C for 10 minutes for gelatinization; add α-cyclodextrin glucosyltransferase with an enzyme activity of 200 units per gram of starch, add The volume is n-decyl alcohol with 5% starch mass, reacted for 6 hours, and stopped the reaction. The reaction product was filtered under airtight conditions, and the insoluble filter cake was washed repeatedly with distilled water to collect the filter cake. Add absolute ethanol with an initial reaction volume of 30%, stir the reaction at room temperature for 5 hours, and put it into a separatory funnel to stand still for 5 hours. Liquid separation and filtration, the supernatant liquid is a mixture of absolute ethanol and n-decyl alcohol, the lower liquid is α-cyclodextrin solution, and the lower liquid is evaporated by a rotary evaporator to obtain α-cyclodextrin. The total conversion rate of corn starch reaches 67.1%, and the proportion ...

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Abstract

The invention relates to a method using ocean alpha-cyclodextrin glucosyltransferase to convert starch so as to prepare alpha-cyclodextrin. The method includes: performing starch gelatinization, adding the alpha-cyclodextrin glucosyltransferase at 55 DEG C, adding n-decanol to perform reaction, filtering reaction products, repeatedly washing filter cake with distilled water, and colleting the filter cake; adding anhydrous ethanol, performing stirring reaction under room temperature for 5 hours, performing static separatory filtration in a separating funnel, and evaporating lower liquid to obtain the alpha-cyclodextrin. The method has the advantages that the method is simple in flow, short in cycle and high in conversion rate, and the obtained alpha-cyclodextrin is high in purity and widely applicable to fields such as food, medicine, agriculture, spinning, environment protection, cosmetics, biotechnology and analytic chemistry.

Description

technical field [0001] The invention relates to the application field of marine biological enzymes, in particular to a method for preparing alpha-cyclodextrin by transforming starch with marine alpha-cyclodextrin glucosyltransferase. Background technique [0002] Cyclodextrin is a kind of cyclic, non-reducing terminal maltooligosaccharides formed by starch or polysaccharide under the action of cyclodextrin glucosyltransferase, connected by α-1,4-glucosidic bonds. Cyclodextrins usually consist of 6, 7 or 8 glucose residues and are called α-, β-, or γ-cyclodextrins, respectively. In addition to these three commonly used cyclodextrins, there are also δ-, ε-, ζ-, and η-cyclodextrins (composed of 9 to 12 glucose units, respectively). Since cyclodextrin can form inclusion complexes with many guest molecules, thereby changing the physical and chemical properties of the guest molecules such as solubility and stability, it is widely used in food, medicine, agriculture, textiles, env...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/04
CPCC12P19/18C12P19/04
Inventor 郝建华孙谧刘均忠
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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