A kind of method for rapid stacking fermentation of green brick tea

A technology of stacking fermentation and fermentation method, which is applied in the field of green brick tea fermentation, to achieve strong adaptability and synergy, increase enterprise benefits, and improve the effect of fermentation speed

Active Publication Date: 2022-02-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the isolation of Aspergillus fumigatus from Pu-erh tea pile fermentation, and the use of Aspergillus fumigatus in the fermentation and processing of green brick tea, and there is no report that only two strains can be used to eliminate the main components of sun-dried green tea within a week. Content of quality ingredients fermented to ideal Odui tea-like levels

Method used

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  • A kind of method for rapid stacking fermentation of green brick tea
  • A kind of method for rapid stacking fermentation of green brick tea
  • A kind of method for rapid stacking fermentation of green brick tea

Examples

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Embodiment 1

[0134] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0135] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 3g / L, peptone 10g / L, and NaCl 5g / L.

[0136] 2. Preparation of Aspergillus fumigatus liquid: place the Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating it for 2-36 hours under t...

Embodiment 2

[0142] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0143] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 4g / L, peptone 15g / L, and NaCl 3g / L.

[0144] 2. Preparation of Aspergillus fumigatus liquid: place the Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating it for 2-36 hours under t...

Embodiment 3

[0150] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0151] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 5g / L, peptone 12g / L, and NaCl 9g / L.

[0152] 2. Preparation of Aspergillus fumigatus liquid: place the Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating it for 2-36 hours under t...

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PUM

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Abstract

The invention provides a method for rapid stacking fermentation of green brick tea, which belongs to the technical field of green brick tea fermentation. The ratio of V) is mixed and put into the fermented pile fermentation container, and then sterilized; inoculate the fermented pile fermented liquid with the inoculum amount of 5-20% (V / W) into the fermented pile fermented container after sterilization. Aerobic culture at 45±3°C. The present invention uses two strains of Bacillus subtilis and Aspergillus fumigatus to carry out pure-bred composting fermentation, not only the product components are the same as the traditional open composting fermentation, but also the flavor is the same, which is different from the natural composting process of microorganisms in the traditional open utilization environment. Compared with this method, the time can be shortened by about 4 weeks, and it is not easy to be contaminated by bacteria, which is conducive to increasing corporate benefits, improving and stabilizing product quality.

Description

technical field [0001] The invention belongs to the technical field of green brick tea fermentation, and in particular relates to a method for rapid pile fermentation of green brick tea. Background technique [0002] Heaping is the key process to form the quality of green brick tea. The traditional heaping of green brick tea is mainly controlled by experience, which greatly limits the guarantee of tea quality and the application of modern fermentation technology. Therefore, it is necessary to understand the microbial community structure during the fermentation process of green brick tea, so as to select and develop potential starters, shorten the fermentation time of fermentation, and achieve clean and standardized production. [0003] The key process for the formation of the quality of green brick tea is heaping and aging in storage. The essence of heaping is to use the microorganisms suspended in the air to naturally grow on the tea dregs to produce extracellular enzymes a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23F3/10C12N1/20C12N1/14C12R1/125C12R1/68
CPCA23F3/10C12N1/14C12N1/20
Inventor 付瑞燕徐倩宁井铭陈军海
Owner ANHUI AGRICULTURAL UNIVERSITY
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