A kind of green brick tea stack fermentation strain composition and application

A technology of tea stacking and composition is applied in the field of green brick tea fermentation to achieve the effects of strong adaptability and synergy, increasing enterprise benefits and improving fermentation speed.

Active Publication Date: 2022-03-04
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the isolation of Aspergillus fumigatus from Pu-erh tea pile fermentation, and the use of Aspergillus fumigatus in the fermentation and processing of green brick tea, and there is no report that only two strains can be used to eliminate the main components of sun-dried green tea within a week. Content of quality ingredients fermented to ideal Odui tea-like levels

Method used

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  • A kind of green brick tea stack fermentation strain composition and application
  • A kind of green brick tea stack fermentation strain composition and application
  • A kind of green brick tea stack fermentation strain composition and application

Examples

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Embodiment 1

[0126] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0127] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 3g / L, peptone 10g / L, and NaCl 5g / L.

[0128] 2. Preparation of Aspergillus fumigatus liquid: place Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating for 2-36 hours under the cond...

Embodiment 2

[0134] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0135] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 4g / L, peptone 15g / L, and NaCl 3g / L.

[0136] 2. Preparation of Aspergillus fumigatus liquid: place Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating for 2-36 hours under the cond...

Embodiment 3

[0142] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0143] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 5g / L, peptone 12g / L, and NaCl 9g / L.

[0144] 2. Preparation of Aspergillus fumigatus liquid: place Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating for 2-36 hours under the cond...

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Abstract

The invention provides a bacterial strain composition for stack fermentation of green brick tea, which belongs to the technical field of microbial strains. The strain composition of green brick tea stack fermentation includes Bacillus subtilis and Aspergillus fumigatus, wherein, the Bacillus subtilis is preserved in China Center for Type Culture Collection, and the preservation number is: CCTCC M 2018288; the Aspergillus fumigatus is preserved in China Typical Culture Collection Center, the collection number is: CCTCC M 2018287. The present invention uses two strains of Bacillus subtilis and Aspergillus fumigatus to carry out pure-bred composting fermentation, not only the product components are the same as the traditional open composting fermentation, but also the flavor is the same, which is different from the natural composting process of microorganisms in the traditional open utilization environment. Compared with this method, the time can be shortened by about 4 weeks, and it is not easy to be contaminated by bacteria, which is conducive to increasing corporate benefits, improving and stabilizing product quality.

Description

technical field [0001] The invention belongs to the technical field of green brick tea fermentation, and in particular relates to a composition and application of green brick tea fermenting strains. Background technique [0002] Green brick tea belongs to black tea, which is a kind of post-fermented tea. It uses dried old green tea as raw material, which is naturally fermented and pressed. It is mainly produced in the Xianning area of ​​Hubei Province and has a production history of more than 100 years. Green brick tea has the functions of eliminating greasiness, reducing fat and losing weight, supplementing vitamins, etc., and can eliminate the health threats caused by the special eating habits of ethnic minorities in the frontier. For a long period of time, green brick tea has been a "policy commodity, fixed-point production, unified purchase and marketing", and an indispensable daily necessities for ethnic minorities in the frontier. [0003] Heaping is the key process ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A23F3/10C12R1/125C12R1/68
CPCA23F3/10C12N1/14C12N1/20
Inventor 付瑞燕徐倩宁井铭陈军海
Owner ANHUI AGRICULTURAL UNIVERSITY
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