The invention discloses spleen-tonifying health biscuits containing pine berries and a preparation method of the biscuits. The biscuits are prepared from raw materials in parts by weight as follows: 2-3 parts of pumpkin powder, 4-5 parts of the pine berries, 2-3 parts of fresh sweet potato leaves, 4-5 parts of oenanthe javanica, 1-2 parts of poria cocos, 1-2 parts of platycodon roots, 1-2 parts of fructus lycii, 200-220 parts of flour, 30-40 parts of fresh mulberry leaves, 1.5-2 parts of yeast, 18-20 parts of white granulated sugar, 10-20 parts of milk, 8-10 parts of corn dietary fiber, 15-18 parts of wheat germ oil, 6-7 parts of eggs, 2-3 parts of salt, 4-5 parts of baking soda and a proper amount of water. Paste is stirred slowly and then quickly, so that the paste is smooth and good in toughness, added auxiliary materials can improve the taste of the biscuits, the health-care function of the biscuits is realized, the paste is fermented properly after two-time mixing and two-time fermentation, becomes uniform and fine and then is rolled and baked moderately, and the prepared biscuits have pure fragrance, tastes crisp, is free of any coarse grain feeling and is excellent in color, flavor and taste.