Bacterial strain composition for pile fermentation of green brick tea and application

A kind of technology of tea stacking and composition, applied in the field of green brick tea fermentation

Active Publication Date: 2018-12-21
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the isolation of Aspergillus fumigatus from Pu-erh tea pile fermentation, and the use of Aspergillus fumigatus in the fermentation and processing of green brick tea,

Method used

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  • Bacterial strain composition for pile fermentation of green brick tea and application
  • Bacterial strain composition for pile fermentation of green brick tea and application
  • Bacterial strain composition for pile fermentation of green brick tea and application

Examples

Experimental program
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Effect test

Embodiment 1

[0126] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0127] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 3g / L, peptone 10g / L, and NaCl 5g / L.

[0128] 2. Preparation of Aspergillus fumigatus liquid: place Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating for 2-36 hours under the cond...

Embodiment 2

[0134] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0135] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 4g / L, peptone 15g / L, and NaCl 3g / L.

[0136] 2. Preparation of Aspergillus fumigatus liquid: place Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating for 2-36 hours under the cond...

Embodiment 3

[0142] This example provides a method for rapid stacking fermentation of green brick tea, which can quickly ferment green brick tea, and has the same main quality and chemical components as traditional green brick tea, and the time is shortened by 80%. The pile fermentation method of the present invention is as follows:

[0143] 1. Preparation of Bacillus subtilis bacteria liquid: place the Bacillus subtilis (CCTCC M 2018288) preserved in the present invention in NA medium for cultivation, and after cultivating for 2 to 36 hours under the condition of 45±3° C., disperse its bacterial cells, Then suspension treatment, to 10 6- 10 7 cells / mL to obtain the Bacillus subtilis liquid. Wherein, the NA medium is: a mixed solution of beef extract 5g / L, peptone 12g / L, and NaCl 9g / L.

[0144] 2. Preparation of Aspergillus fumigatus liquid: place Aspergillus fumigatus (CCTCC M 2018287) preserved in the present invention in PDA medium, and after cultivating for 2-36 hours under the cond...

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Abstract

The invention belongs to the technical field of microbial strains. and provides a bacterial strain composition for the pile fermentation of green brick tea, comprising bacillus subtilis and aspergillus fumigatus, wherein the bacillus subtilis is reserved in China Center for Type Culture Collection with the preservation number of CCTCC M 2018288 and the aspergillus fumigatus is reserved in China Center for Type Culture Collection with the preservation number of CCTCC M 2018287. By the pure strain pile fermentation with the two strains, i.e., the bacillus subtilis and the aspergillus fumigatus,not only product ingredients but also flavor are the same as those by traditional open pile fermentation, 4 weeks can be shortened or so than that by a traditional open type natural pile fermentationprocess that makes use of microorganisms in the environment, and infectious microbe pollution is less, thus being beneficial to increasing enterprise benefit and promoting and stabilizing product quality.

Description

technical field [0001] The invention belongs to the technical field of green brick tea fermentation, and in particular relates to a composition and application of green brick tea fermenting strains. Background technique [0002] Green brick tea belongs to black tea, which is a kind of post-fermented tea. It uses dried old green tea as raw material, which is naturally fermented and pressed. It is mainly produced in the Xianning area of ​​Hubei Province and has a production history of more than 100 years. Green brick tea has the functions of eliminating greasiness, reducing fat and losing weight, supplementing vitamins, etc., and can eliminate the health threats caused by the special eating habits of ethnic minorities in the frontier. For a long period of time, green brick tea has been a "policy commodity, fixed-point production, unified purchase and marketing", and an indispensable daily necessities for ethnic minorities in the frontier. [0003] Heaping is the key process ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A23F3/10C12R1/125C12R1/68
CPCA23F3/10C12N1/14C12N1/20
Inventor 付瑞燕徐倩宁井铭陈军海
Owner ANHUI AGRICULTURAL UNIVERSITY
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