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Method for preparing alpha-glucosylhesperidin

A technology of glucosyl and hesperidin, which is applied in the field of preparation of modified hesperidin, can solve the problems that other functions of hesperidin have not been evaluated, and achieve the effects of high yield, mild reaction conditions and simple route

Inactive Publication Date: 2011-05-11
成都丸荣易康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 3. Sulfonation of hesperidin: The sulfonation method of hesperidin is worth the hesperidin sulfonation product as a mixture of five sodium hesperidin sulfonates. Through sulfonation reaction, its water solubility is improved, but the Other functions of hesperidin have not been evaluated and need further research

Method used

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  • Method for preparing alpha-glucosylhesperidin
  • Method for preparing alpha-glucosylhesperidin
  • Method for preparing alpha-glucosylhesperidin

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1: Mix 5.0 g of hesperidin with 20 ml of 5% dextrin, then add 2.5 ml of α-glucosyltransferase, adjust the pH value to about 6.0, and react at 55° C. for 24 hours. HPLC detection showed that hesperidin was partially transformed into α-glucosyl hesperidin. After the reaction was completed, the reaction mixture was heated to 80°C to inactivate the enzyme (referring to α-glucosyltransferase, the same below), and after standing overnight, it was filtered to obtain the supernatant. The supernatant is passed through HP-10 macroporous resin column, and then decolorized by activated carbon filtration (washing the column with distilled water to remove sugar and inorganic salts, and then washing the column with 70% ethanol solution), concentrated and crystallized to obtain the product α-glucosyl Hesperidin, the conversion rate of hesperidin in this process is 52.54%.

Embodiment 2

[0038] Example 2: Mix 5.0 g of hesperidin with 20 ml of 5% dextrin, add 3.0 ml of α-glucosyltransferase, adjust the pH value to about 6.0, and react at 55° C. for 24 hours. HPLC detection showed that hesperidin was partially transformed into α-glucosyl hesperidin. After the reaction, the reaction mixture was heated to 80°C to inactivate the enzyme, then the solvent (referring to water, the same below) was spin-dried, and then distilled water was added, and the supernatant was obtained by filtration after standing overnight. The supernatant was passed through a HP-10 macroporous resin column, then decolorized by activated carbon filtration (washing the column with distilled water to remove sugar and inorganic salts, and then washing the column with 70% ethanol solution), concentrated and spin-dried to obtain the product α-glucose Base hesperidin, the conversion rate of hesperidin in this process is 59.41%.

Embodiment 3

[0039] Example 3: Mix 5.0 g of hesperidin with 20 ml of 5% dextrin, add 3.0 ml of α-glucosyltransferase, adjust the pH value to about 7.0, and react at 60° C. for 24 hours. HPLC detection showed that hesperidin was partially transformed into α-glucosyl hesperidin. After the reaction, the reaction mixture was heated to 80°C to inactivate the enzyme, then the solvent was spin-dried and distilled water was added, and the supernatant was obtained by filtration after standing overnight. The supernatant is passed through HP-10 macroporous resin column, and then decolorized by activated carbon filtration (washing the column with distilled water to remove sugar and inorganic salts, and then washing the column with 70% ethanol solution), concentrated and crystallized to obtain the product α-glucosyl Hesperidin, the conversion rate of hesperidin in this process is 66.47%.

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Abstract

The invention relates to a method for preparing alpha-glucosylhesperidin. The method comprises the following steps of: (a) enzymatic conversion reaction: carrying out an alpha-glucosyl reaction on hesperidin and dextrin which are used as raw materials under the action of alpha-glucosyltransferase to generate alpha-glucosylhesperidin; (b) resin adsorption and purification: after the reaction of the step (a) ends, heating a reaction mixed solution to 80 DEG C to inactivate enzymes, placing over the night, filtering, taking a supernate and treating the supernate through an HP-10 macroporous resin column to remove sugar and inorganic salts; (c) decoloring: decoloring the supernate treated through the macroporous resin column with active carbon; and (d) concentration and crystallization: concentrating and crystallizing the liquid decolored with the active carbon in the step (c) to obtain a target product. The method has the advantages of simple route, moderate reaction condition and high yield.

Description

technical field [0001] The invention relates to a preparation method of modified hesperidin, in particular to a preparation method of α-glucosyl hesperidin. Background technique [0002] Hesperidin is one of the 60 kinds of flavonoids in citrus. It is mainly distributed in citrus plants and often exists in Rutaceae plants such as sweet orange, lime, lemon, orange, grapefruit and citrus. Hesperidin is a dihydroflavone with a melting point of 245-254°C. It is weakly acidic, hardly soluble in water, almost insoluble in acetone, benzene, and chloroform, and easily soluble in dilute alkali solutions. Hesperidin can resist oxidation, can completely inhibit the formation of lipid peroxides, has obvious scavenging effect on hydroxyl free radicals, has anti-cancer, anti-virus, anti-bacterial effects, and has broad-spectrum antibacterial effects on common food contamination bacteria It also has the effect of strengthening capillaries and preventing capillary bleeding. It is an effect...

Claims

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Application Information

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IPC IPC(8): C12P19/60
Inventor 高兰
Owner 成都丸荣易康科技有限公司
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