32 results about "Staphylococcus salivarius" patented technology

A process for enzymatic preparation of a highly linear poly(α 1, 3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from Streptococcus salivarius is used to convert sucrose to a highly linear poly(α 1, 3 glucan) in high titers. Hydrolyzed poly(α 1, 3 glucan) is used as the primer for the gtfJ enzyme reaction resulting in the formation of highly linear poly(α 1, 3 glucan).

This invention provides novel Streptococcus salivarius, compositions containing same, and use of S. salivarius strains as antimicrobial agents. The strains are bacterial inhibitors with respect to at least S. mutans and/or MS and therefore have a number of therapeutic applications. The applications include but are not limited to forming part of therapeutic formulations for use in controlling, treating, or preventing dental caries.

The invention discloses intestinal microorganism markers for detecting multiple myeloma diseases, and an application and detection preparation of the intestinal microorganism markers. The intestinal microorganism markers comprise citrobacter freundii, enterobacter cloacae, raoultella ornithinolytica, klebsiella aerogenes, klebsiella variicola, klebsiella pneumoniae, streptococcus salivarius, streptococcus oralis, streptococcus gordonii, streptococcus mitis, streptococcus pneumoniae, butyrate producing bacteria, clostridium butyricum and clostridium saccharobutylicum. The intestinal microbial markers can be used alone or in combination. The invention discloses a support vector machine (SVM) classification model constructed by taking the microbial markers as characteristics, and the SVM classification model is applied to clinical diagnosis of multiple myeloma diseases. The invention also discloses an application of the microorganism markers in preparation of a diagnostic preparation formultiple myeloma diseases.

The invention discloses a kit for detecting colorectal cancer indicator bacteria. The kit is characterized by comprising the following components: a specific primer combination for identifying the indicator bacteria, wherein the primer combination is shown as SEQ ID NO. 1-14, and the indicator bacteria are the combination of fusobacterium nucleatum (F.nucleolus), anaerobic digestion streptococcus(P.anaerobicus), clostridium symbiosum (C.symbiolum), Porphyromonas saccharolytica (P.asaccharolytica), Prevotella intermedia (P.intermedia), Bacteroides fragilis (B.fragilis) and Streptococcus salivarius (S.salivarius). The kit for detecting colorectal cancer indicator bacteria disclosed by the invention has the beneficial effects that the colorectal cancer can be identified only by collecting astool sample. The principle is that the aim of identifying the colorectal cancer by detecting the colorectal cancer pathogenic bacteria in excrement, the sensitivity can reach 92.9%, and the specificity is 92.6%.

The invention belongs to the technical field of probiotic products and relates to a probiotic solid beverage facilitating oral health. The solid beverage comprises the following ingredients by mass percentage: 20-30wt% of lemon extract, 10-20wt% of inulin, 15-20wt% of streptococcus salivarius, 15-20wt% of lactobacillus salivarius and 15-20wt% of lactobacillus acidophilus. Three probiotics adoptedby the beverage can protect the oral health and prevent oral diseases by inhibiting growth and reproduction of pathogens and competing adhesion sites on the surface of an oral mucosa with the pathogens; the inulin can improve a flavor of the product, promote propagation of the probiotics and absorb water from the product; the probiotics are higher in stress resistance and higher in survival rate,so that the product has a longer shelf life; and the lemon extract is fresh in smell, allows the solid beverage to have a unique flavor, can quickly dissipate an oral peculiar smell, is rich in vitamin B and vitamin C, can prevent gum bleeding and dental ulcers and makes gingivae strong.

The invention relates to the technical field of microbial preparations, in particular to a probiotic microecological preparation for treating oral halitosis and inflammation as well as a preparation method and application thereof. The probiotic microecological preparation comprises the following raw materials in parts by weight: 5-80 parts of probiotics, 1-40 parts of a functional sweetening agent, 1-20 parts of maltodextrin and 2-10 parts of freeze-dried fruit powder, wherein. the probiotics comprise streptococcus salivarius k12. All the component substances are compounded to generate a synergistic effect, so that various nutrients are provided for consumers, the probiotic effects of better protecting oral cavity health care and the like are achieved, and an important effect is achieved on prevention of oral diseases such as periodontitis, decayed teeth, halitosis and oral ulcer.

This invention relates to a urase gene of streptococcus salivarius, concretely, concerning about a 57.I urase gene of streptococcus salivarius and its urase. A 57.I urase gene of streptococcus salivarius , whose nucleotide sequence is what is described in SEQ ID NO.1. Nickel ion bonding in the gene and nucleotide sequence of transit-related gene MQO are what are described in SEQ ID NO.2. The advantage of this invention is that gaining the cloning of urase gene of streptococcus salivarius U35248 by the method of segment cloning and gradually enzyme-cutting connection ,and its object gene length proceeds to 8Kb, increasing Nickel ion bonding of the gene and transit-related gene MQO compared with existing technology , therefore, expressing ureolytic activity without adding exogenous NiCl2.Attained cloning can be used for future replacement therapy in anti-caries research building without adding exogenous NiCl2,more suitable for clinical application of production alkali effect germ.

The invention provides streptococcus salivarius and an application thereof to preparation of medicines for treatment of inflammatory bowel diseases, and relates to the field of a biological technology. The streptococcus salivarius (streptococcus salivarius) 1802 is preserved in the preservation center with the preservation number of CCTCC NO:M 2019251. A screening method of the streptococcus salivarius comprises the steps of enabling excrement of healthy people to be subjected to suspension with bacteria-free water, performing diluting until the volume is 10-6 times, and performing fractionalcultivation in a brain-heart leaching solution culture medium with a plate streak to obtain the streptococcus salivarius (streptococcus salivarius) 1802. The streptococcus salivarius (streptococcus salivarius) 1802 can be applied to preparation of medicines for treatment of inflammatory bowel diseases, can be used in medicines for relieving inflammatory reactions of the inflammatory bowel diseases, and can be applied to medicines for relieving inflammatory bowel disease colon mucous membrane injury.

The invention discloses a preparation method for a triclosan-containing polyethylene glycol-b-poly(beta-urethane-urea) polymer micelle and application of the micelle in selectively killing an oral streptococcus mutans of a biofilm. The polyethylene glycol-b-poly(beta-urethane-urea) polymer is obtained by performing Michael addition reaction on triclosan-containing monomer molecues. The triclosan-bonded polyethylene glycol-b-poly(beta-urethane-urea) polymer has relatively good killing effect on the harmful bacteria oral streptococcus mutans of the biofilm, and can selectively keep oral beneficial bacterium streptococcus salivarius. The micelle system has the advantages that: firstly, preparation is simple; secondly, large-scale preparation can be realized, and conversion to the clinical direction is easy; thirdly, biofilm infiltration capacity is good; and fourthly, selective killing ability on harmful bacteria is achieved.

The invention discloses a fermented feed for laying hens as well as a preparation method and application of the fermented feed. The preparation method comprises the following steps: sequentially carrying out hydrogen peroxide oxidation and ammonia water pH adjustment on corn steep liquor, uniformly mixing the corn steep liquor with corn bran and wheat bran subjected to steam conditioning treatmentto obtain a raw material mixture, inoculating Baillard yeast and bacillus subtilis, performing aerobic fermentation, then inoculating streptococcus salivarius, performing anaerobic fermentation, andfinally, performing pneumatic drying to obtain the fermented feed. According to the preparation method of the fermented feed for the laying hens, hydrogen peroxide is adopted to treat the corn steep liquor, and sulfite in the corn steep liquor is oxidized, so that adverse effects on animal physiology are eliminated; the corn steep liquor is treated by adopting hydrogen peroxide and ammonia water,and the wheat bran after conditioning treatment with high-temperature steam is combined for use, so that the raw material mixture can be suitable for solid aerobic fermentation of Baillard yeast and bacillus subtilis; and a finished product obtained through combined fermentation of aerobic fermentation and anaerobic fermentation is a good laying hen feed raw material, so that the additional valueof corn starch byproducts is greatly improved, and obvious economic benefits and ecological benefits are realized.

The invention discloses an acute coronary syndrome risk assessment marker for a stable coronary heart disease and application. The markers comprise intestinal flora bifidobacterium longum, streptococcus angora, companion faecalis, Kolinella aerogenes, faecalis przewalskii, bacteroides ovale, anaerocanoidea, bacteroides fragilis, bacteroides holdmann, eubacterium rectum and streptococcus salivarius. According to the invention, risk assessment prediction is carried out on the acute coronary syndrome of the stable coronary heart disease, and the prediction accuracy can be improved.

The invention relates to an enzymatic preparation method of glucosamine, and belongs to the technical field of bioengineering. The method comprises the following steps: converting D-glucose into D-fructose by adopting glucose isomerase catalysis; and converting D-fructose into glucosamine using transaminase, an amino donor compound and a weak oxidant compound, wherein the glucose isomerase is derived from bacillus coagulans, flavobacterium dendrimer, streptomyces olivaceus, bacillus coagulans, flavobacterium dendrimer and streptomyces olivaceus; and the transaminase is derived from bacillus subtilis, bacillus velezensis, bacillus pumilus, bacillus licheniformis, streptococcus salivarius and lactobacillus masi. According to the method for preparing the glucosamine by catalyzing the D-glucose in vitro by utilizing the biological enzyme, the initial raw materials are glucose isomerase and transaminase required by the D-glucose or D-fructose, and the final product glucosamine can be directly obtained by constructing escherichia coli genetic engineering expression bacteria and catalyzing by adopting a 'one-pot method'. The method has the advantages of cheap raw materials, low production cost, environmental friendliness and the like.

The invention provides milk-derived streptococcus salivarius for predicting infant obesity and a separation method of the milk-derived streptococcus salivarius. The milk-derived streptococcus salivarius is a streptococcus salivarius strain SHDP5A1, a 16SrRNA gene sequence of the milk-derived streptococcus salivarius strain is shown as SEQ ID NO: 1, the milk-derived streptococcus salivarius strain is classified and named as Streptococcus salivarius, the preservation number is CCTCCNO:M2021060, the preservation date is January 14, 2021, the preservation unit is China Center for Type Culture Collection, and the preservation unit address is Wuhan University, Wuhan, China. The milk-derived streptococcus salivarius for predicting the infant obesity, provided by the invention, serves as facultative anaerobic bacteria, is easy to colonize in intestinal tracts, and creates conditions for maturation and stabilization of intestinal flora in a later period, so that prediction of filial generation obesity can be realized through abundance detection of streptococcus salivarius in an early life period.

The use of orally administrable compositions comprising Streptococcus salivarius is described, for the prevention or the treatment of psoriasis in all its forms. In particular, the present invention relates to the use of compositions comprising Streptococcus salivarius, for the treatment of psoriasis in all its forms.

The invention provides a primer group for detecting streptococcus salivarius, a detection system and applications of the primer group and the detection system. The primer group is a specific primer designed for 16S rRNA (ribosomal ribonucleic acid) of intestinal bacteria, and the specific primer comprises 3-5 random bases. The detection system is explored and verified based on the primer group; steps and conditions are mutually matched to achieve synergic enhancement; a detection result of the detection system is stable and accurate; the detection steps are concise and efficient; and wide application prospects and large market values are achieved.

The invention provides a probiotic composition suitable for oral cavity, and relates to the technical field of oral cavity health care. The probiotic composition suitable for the oral cavity is prepared from the following raw materials in parts by mass: 10 to 30 parts of microcapsules, 60 to 70 parts of probiotic powder, 10 to 40 parts of plant powder, 5 to 10 parts of isomaltooligosacharide, 5 to 15 parts of fructo-oligosaccharide and 20 to 30 parts of starch, the probiotic powder comprises the following raw materials in parts by weight: 1-10 parts of lactococcus lactis, 1-5 parts of streptococcus salivarius, 1-5 parts of bifidobacterium, 1-10 parts of lactobacillus casei, 1-10 parts of lactobacillus plantarum, 1-10 parts of lactobacillus acidophilus, 1-5 parts of streptococcus thermophilus, 2-5 parts of chitosan, 2-5 parts of lactic acid and 2-15 parts of honey. The probiotic composition is placed in the oral cavity, the probiotic powder and the plant powder can be released to play a role by chewing the probiotic composition, and the taste of the plant powder can be neutralized through the fructo-oligosaccharide, so that the probiotic composition is rich in taste, sweet in taste and convenient to use for many times at high frequency in daily life, and the treatment effect on oral problems is improved.

The invention belongs to the technical field of microorganisms, and discloses a probiotics-containing care lotion for external use. The probiotics-containing care lotion for external use is prepared by mixed fermentation of Streptococcus salivarius, Lactobacillus acidophilus and Lactobacillus rhamnosus. The care lotion for external use is a pure microecological preparation, has a good antibacterial effect but has no side effects.