Lentinan powder for preventing and/or assisting in treating tumors and extraction process thereof

A technology of lentinan and an extraction method is applied in the directions of antitumor drugs, medical raw materials derived from fungi, medical preparations containing active ingredients, etc. The effect of improving the tumor microenvironment

Inactive Publication Date: 2018-12-25
陶宁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has also been found that harsh extraction conditions such as strong bases, high pressure, and ultrasound can lead to fragmentation of the extracted lentinan, thereby reducing the molecular weight or inactivating the compound

Method used

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  • Lentinan powder for preventing and/or assisting in treating tumors and extraction process thereof
  • Lentinan powder for preventing and/or assisting in treating tumors and extraction process thereof
  • Lentinan powder for preventing and/or assisting in treating tumors and extraction process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The process for extracting lentinan powder for preventing tumors of the present invention comprises:

[0032] 1. Take 1kg of mushrooms (Lentinus edodes (Berk.) Sing.), add 10L of water, and extract in boiling water for 3 hours, a total of 2 extractions. Each extract was filtered and combined, heated and concentrated to a small volume, dialyzed against flowing water for 48 hours, concentrated the dialyzed liquid and centrifuged, added 30% trichloroacetic acid solution 1:1 (v / v), at 4°C React under conditions for 4 hours, add 10% NaOH solution to neutralize to about pH 7, tie a bag and dialyze against flowing water for 48 hours, concentrate the inner liquid of the dialysate and centrifuge, add 3 times the volume of 95% ethanol to the supernatant, and let it stand overnight. The precipitate was collected by centrifugation and dried at 40°C to obtain crude polysaccharide.

[0033] 2. Melt 10 g of the crude polysaccharide extracted in step 1 into 1 L of water, add 1 L of et...

Embodiment 2

[0039] Example 2: Reversal effect of lentinan LE58 extracted by the present invention on immunosuppressive cells

[0040] as attached figure 1 Shown are all flow cytometry diagrams, figure 1 Middle (a) is normal splenocytes; figure 1 Middle (b) is the proliferation peak of CD4+ T cells in splenocytes stimulated by concanavalin (ConA); figure 1 Middle (c) is immunosuppressive cells (myeloid-derived suppressor cells) inhibiting the proliferation of CD4+ T cells; figure 1 In (d) lentinan LE58 restored the proliferation of CD4+ T cells.

[0041] The splenocytes of BALB / c mice were prepared into a single cell suspension, and seeded in a 96-well cell culture plate (U-shaped bottom), the cell seeding density was 3×105 / well, and ConA and myeloid-derived suppressor cells were added in turn. For MSC2 and lentinan LE58, the concentration of ConA is 1.5 μg / well, the number of MSC2 cells is 9×103 / well, and the concentration of polysaccharide LE58 is 0.2 mg / ml. After 3 days of culture,...

Embodiment 3

[0042] Example 3: In vivo inhibitory effect of lentinan LE58 extracted by the present invention on lung cancer

[0043] as attached figure 2 As shown, the line corresponding to the diamond icon is the survival curve of the lung cancer tumor-bearing mice in the control group; the line corresponding to the rectangular icon is the survival curve of the lung cancer tumor-bearing mice in the lentinan LE58 group. LLC Lewis lung cancer cells were inoculated subcutaneously in the abdomen of C57 mice, the number of inoculated cells was 1×106 / mouse, and the subcutaneous xenograft tumor model of lung cancer was constructed. After 10 days, the tumor formed, and the intraperitoneal injection was performed with lentinan LE58 at a dose of 25 mg / kg body weight . The comparison between the two shows that the present invention has a certain inhibitory effect on the growth of lung cancer.

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Abstract

The invention provides lentinan powder for preventing and / or assisting in treating tumors and an extraction process thereof. The invention provides an extraction method of lentinan. The method includes a step of contacting a solution containing lentinan or crude-extracted lentinan powder with alcohol solutions with different concentrations to precipitate lentinan. The invention further provides lentinan obtained by the method and an application thereof in preparing medicines for preventing and / or treating the tumors. The invention further provides a medicine or a food composition containing the lentinan obtained by the method. The lentinan obtained by the method of the invention has an excellent effect of preventing and treating the tumors.

Description

technical field [0001] The invention relates to new lentinan powder and its extraction process. Specifically, the present invention relates to a new lentinan powder with the activity of preventing and / or adjuvant treating tumors and its extraction process. Background technique [0002] In view of the high incidence of tumors and the limited means of prevention and treatment, there is an urgent need in the market for a preparation for the prevention and / or adjuvant treatment of tumors, such as a preparation derived from food, to obtain the effect of preventing and treating tumors. [0003] There is currently a lack of cancer prevention products on the market. Most of the existing products aim to directly kill tumor cells. However, while they kill tumor cells, they often kill immune effector and immune memory cells. There are also some products that target positive immune effects and strengthen effector immune cells, which is often said to improve immunity. However, modern...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCA61K31/715A61K36/07C08B37/0003
Inventor 陶宁方爱平
Owner 陶宁
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