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Device and method for biologically manufacturing succinic acid by continuous crystallization

A bio-manufacturing, succinic acid technology, applied in the field of succinic acid fermentation and separation and purification, can solve the problems of low time yield, low production efficiency, lower product concentration, etc. Effect

Active Publication Date: 2012-08-15
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the production process of succinic acid, fermentation is usually carried out first, membrane filtration is carried out after fermentation, crystallization is carried out, and purification is carried out again. Moreover, during the fermentation process of succinic acid, the accumulation of succinic acid will inhibit the fermentation process and reduce the product concentration, which is an important factor hindering the industrialization of succinic acid fermentation; at the same time, because each part of the succinic acid device is independent, resulting in The production of succinic acid cannot be carried out continuously
The process of producing succinic acid by the existing microbial fermentation method is mostly intermittent operation, that is, after the fermentation is completed, the tank is put into the tank, and the feed liquid in the tank enters the downstream separation and purification chemical unit operation, which has a long cycle and low time yield; Isolated, usually one unit is overloaded while other units are shut down, and the production efficiency is very low
In the process of preparing succinic acid, other by-product organic acids, succinate ions (succinate) and protein will inevitably be produced, and the production of these by-products will also inhibit the fermentation system of succinic acid, limiting The development of succinic acid industry

Method used

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  • Device and method for biologically manufacturing succinic acid by continuous crystallization
  • Device and method for biologically manufacturing succinic acid by continuous crystallization
  • Device and method for biologically manufacturing succinic acid by continuous crystallization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Such as figure 1 The bioreactor shown is a fermenter with automatic temperature control, pH adjustment, and feeding. The microorganism selected for fermentation is Actinobacillus succinogenes, also known as 130Z T , deposited in the American Type Culture Collection (ATCC), number ATCC55618. The preparation of the medium: the preservation medium and the activation medium are nutrient-rich TSB medium; the seed medium formula (unit: g / L) glucose 10, yeast powder 6, NaCl 1, MgCl 2 2. CaCl 2 1.5, Na 2 HPO 4 2.9, NaH 2 PO 4 2.2; Seed medium formula (unit: g / L) glucose 30, yeast powder 15, urea 2, KCl 4, MgCl 2 2. MnCl 2 0.07, CaCl 2 1.5, Na 2 HPO 4 4.4, NaH 2 PO 4 3.3, adjust the pH value to 6.4-6.9, and sterilize at 121°C for 30 minutes.

[0055] Under aseptic operation conditions, seed liquid is added to the fermentation liquid, and the volume percentage of the inoculum is 5-7%. At this time, the amount of succinic acid is 0, the stirring is started, the rota...

Embodiment 2

[0059] By adjusting the acidity of the crystallization tank system, the distribution percentages of succinic acid and succinate, by-product organic acids (formic acid, lactic acid, acetic acid) and their salts can be controlled. The degree of dissociation of carboxylic acids in different pH environments is calculated according to their respective dissociation constants, such as acetic acid (K a =6.2×10 -5 ), formic acid (K a =2.1×10 -4 ), lactic acid (K a =1.37×10 -4 ) succinic acid (K a1 =2.1×10 -4 , K a2 =2.3×10 -6 ). The free acid / acid ion distribution diagram of a monobasic acid is calculated according to the following formula:

[0060] δ HA = [ HA ] [ HA ] + [ A - ] = 1 ...

Embodiment 3

[0069] The solubility determination of succinic acid and succinate is carried out under normal pressure, and the temperature is controlled at 0-100°C. The solubility of succinic acid and succinate is calculated according to saturated succinic acid / succinate under specific conditions concentration in solution. HCl and NaOH are used to adjust the mother liquor pH. Such as figure 2Shown is the solubility distribution of succinic acid at different pH at 25°C (room temperature), the solubility of succinic acid in different pH ranges at room temperature, with the decrease of pH, the solubility of succinic acid also drops sharply. Formic, acetic, and lactic acids, whether as acid molecules or acid radical ions, are miscible with water in all pH ranges above freezing; image 3 shows the solubility distribution of succinic acid at different temperatures, Figure 4 shows the solubility distribution of sodium monohydrogen succinate at different temperatures, Figure 5 The solubility...

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Abstract

The invention relates to a device and a method for biologically manufacturing succinic acid by continuous crystallization, and the device comprises: a bioreactor (1), a cross-flow membrane module (2), and a crystallization separation tank (3); a front end of the cross-flow membrane module (2) is communicated with the bioreactor (1) through a pipeline; a filtering end of the cross-flow membrane module (2) is communicated with a bottom of the crystallization separation tank (3); a top of the crystallization separation tank (3) is communicated with the bioreactor (1); therefore, a loop is formed in the device, and the continuous fermentation and crystallization of succinic acid is realized; a peristaltic pump (9) is disposed between the bioreactor (1) and the cross-flow membrane module (2); a back end of the cross-flow membrane module (2) is communicated with the bioreactor (1), which realizes the circulation of the flowing fermentation liquid between the two. The device and the method for biologically manufacturing succinic acid by continuous crystallization of the invention establish a coupling system for low-temperature crystallization in-situ extraction of succinic acid and fermentation and separation, and meet requirements for disinfection sterilization and pollution-free operation.

Description

technical field [0001] The invention relates to the field of succinic acid fermentation and separation and purification. Specifically, the invention relates to a device and method for continuous crystallization of biological production of succinic acid. Background technique [0002] Succinic Acid (Succinic Acid, also known as Succinic Acid, Butanedioic Acid) is recognized as "Generally Recognized as Safe" (GRAS) by the US FDA, so it can be used for a variety of purposes. A 2004 report by the U.S. Department of Energy pointed out that succinic acid is a platform chemical substance for the synthesis of bulk daily necessities and important chemicals. . [0003] The cost of succinic acid is mainly composed of fermentation process and separation cost, among which the cost of separation and purification accounts for more than 60% of the total production cost. How to reduce the cost of fermentation and separation is a research hotspot at home and abroad, and a certain number of pa...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12P7/46C07C55/10C07C51/43
CPCC12M47/12C12M29/18C12M47/10C12M27/02
Inventor 李强邢建民王丹宋子煜张云剑
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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