Method for producing fatty acid by hydrolyzing lipid through three liquid-phase lipase catalytic systems

A three-phase, lipase technology is applied in the field of separation of lipase and lipase hydrolysis of lipids to produce fatty acid, which can solve the problems of low product inhibition reaction efficiency, high lipase separation cost, difficult continuous production, etc. Industrialization, reduction of enzyme loss and purification cost, effect of cost reduction

Active Publication Date: 2013-07-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high separation cost of lipase, difficulty in continuous production, low reaction efficiency and long reaction time caused by product inhibition in the current process of enzymatic hydrolysis of lipids to produce fatty acids. Method for separating lipase from crude enzyme solution and lipase hydrolyzing lipid to produce fatty acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take an appropriate amount of lipase AY30 in a conical flask, add 10 times the volume of water, mix well, take 1.2g of crude enzyme solution (410U / ml), add 0.4g of ammonium sulfate, mix well and add 0.4g polyethylene Diol 400 and 0.4 g of olive oil were put into a stoppered Erlenmeyer flask, mixed evenly, placed on a constant temperature shaker with a rotation speed of 200 rpm, and reacted at 37° C. for 24 hours. Take another 1.2g of crude enzyme solution, add 0.4g of olive oil, and dilute to the same volume with water, and react under the same conditions as a control. After the reaction was finished, let it stand for 0.5h. The experimental group was divided into three phases, and the control group was divided into two phases. The upper phase was taken to measure its fatty acid content, the middle phase of the three liquid phases was taken to measure its enzyme activity, and the middle and lower phases were taken to measure its fatty acid content. The distribution of gl...

Embodiment 2

[0026] Take an appropriate amount of lipase TL100L in a conical flask, add 10 times the volume of water, mix well, take 1.2g of crude enzyme solution (1300U / ml), add 0.4g of ammonium sulfate, mix well and add 0.4g polyethylene Diol 600 and 0.4 g of olive oil were put into a stoppered Erlenmeyer flask, mixed evenly, placed on a constant temperature shaker with a rotation speed of 200 rpm, and reacted at 37° C. for 36 h. Another 1.2 g of crude enzyme solution and 0.4 g of olive oil were taken, diluted with water to the same volume, and reacted under the same conditions as a control. After the reaction was finished, let it stand for 0.5h. The experimental group was divided into three phases, and the control group was divided into two phases. The upper phase was taken to measure its fatty acid content, the middle phase of the three liquid phases was taken to measure its enzyme activity, and the middle and lower phases were taken to measure its fatty acid content. The distribution ...

Embodiment 3

[0028] Take an appropriate amount of lipase PL20000L in a conical flask, add 10 times the volume of water, mix well, take 1.2g of crude enzyme solution (920U / ml), add 0.4g of ammonium sulfate, mix well and add 0.4g polyethylene Diol 2000 and 0.4 g of olive oil were put into a stoppered Erlenmeyer flask, mixed evenly, placed on a constant temperature shaker with a rotation speed of 200 rpm, and reacted at 37° C. for 36 h. Another 1.2 g of crude enzyme solution and 0.4 g of olive oil were taken, diluted with water to the same volume, and reacted under the same conditions as a control. After the reaction was finished, let it stand for 0.5h. The experimental group was divided into three phases, and the control group was divided into two phases. The upper phase was taken to measure its fatty acid content, the middle phase of the three liquid phases was taken to measure its enzyme activity, and the middle and lower phases were taken to measure its fatty acid content. The distributio...

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Abstract

The invention discloses a method for producing fatty acid by hydrolyzing lipid through three liquid-phase lipase catalytic systems. The method comprises the following steps: firstly, preparing enzyme solution with the lipase concentration of 5-20000 U/mL, and adding soluble inorganic salts, alcohol/ketonic hydrophilic fraction and lipid to the enzyme solution so as to form three liquid-phase systems; secondly, laying the prepared three liquid-phase systems into an agitated vessel for reaction, standing or centrifuging the systems after the reaction so as to divide the systems into an upper layer, a middle layer and a lower layer, and collecting products in the upper layer so as to obtain fatty acid phase. The method adopts the three liquid-phase systems to extract fatty acid in crude enzyme solution, performs direct hydrolyzing to produce fatty acid, can not only greatly reduce the production cost of enzyme, but also solve the problems of high energy consumption for glycerol and enzyme separation, high cost, high pollution and the like.

Description

technical field [0001] The invention belongs to the field of bioengineering and food technology, and relates to enzyme separation and application technology, in particular to a method for separating lipase from crude enzyme liquid and lipase hydrolyzing lipids to produce fatty acids. Background technique [0002] As the most important basic raw material in the field of oleochemicals, fatty acids are widely used in soaps, surfactants, food and medicine. At present, the annual output of fatty acids exceeds 3.5 million tons, and its main industrial production route is through the hydrolysis of fats and oils. The former requires high temperature and high pressure, high energy consumption, and many by-products, and is not suitable for the hydrolysis of oils containing hydroxyl fatty acids, polyunsaturated fatty acids, heat-sensitive fatty acids, etc.; while alkaline hydrolysis not only consumes a lot of energy, but also consumes a lot of acid and alkali. and water resources. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 王永华李志刚杨博王卫飞
Owner SOUTH CHINA UNIV OF TECH
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