Comprehensive extraction method for fructus gardeniae
A technology of comprehensive extraction and water gardenia, applied in chemical instruments and methods, steroids, food science, etc., can solve the problems of complicated process and failure of comprehensive extraction and utilization of active ingredients, and achieve high color value and bright color of pigment , the effect of high yield
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[0050] Example 1
[0051] The water gardenia is washed and dried, then crushed through a 40-mesh sieve to obtain water gardenia powder, and then added with water with a solid-to-liquid ratio (w / v) of 1:5, and extracted once at an ultrasonic power of 100W and a temperature of 30℃, 1h Afterwards, the extract was passed through a centrifuge (rotating speed: 8000 rpm, 5 min) for solid-liquid separation, and the filtrate was collected to obtain a filter residue. The filtrate was first filtered with an inorganic ceramic microfiltration membrane (MF) with a pore size of 0.5μm, operating temperature 30℃, pressure: 0.4Mpa; the resulting clear liquid (geniposide concentration 1.6mg / mL) was combined with mesoporous molecular sieve SBA-15 The immobilized β-glucosidase (0.937U / mL, pH5.0) as a carrier was mixed as an aqueous phase-n-octanol as an organic phase (compared to 1:1), and hydrolyzed at 40°C for 40 min (100 rpm). Add 0.05mol / L glycine solution (v / v2:1) to the extracted organic phase...
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[0054] Example 2
[0055] The water gardenia was washed and dried and then crushed through a 40-mesh sieve to obtain water gardenia powder, and then added with water with a solid-to-liquid ratio (w / v) of 1:15, and extracted 3 times at an ultrasonic power of 200W and a temperature of 50℃. After 1 hour, the extract is passed through a centrifuge (rotation speed: 15000 rpm, 20 min) for solid-liquid separation, and the filtrate is collected to obtain a filter residue. The filtrate was first filtered with an inorganic ceramic microfiltration membrane (MF) with a pore size of 0.5μm, operating temperature 60℃, pressure: 0.9Mpa; the resulting clear liquid (geniposide concentration 1.8mg / mL) was combined with mesoporous molecular sieve SBA-15 The immobilized β-glucosidase (4.347U / mL, pH5.0) as a carrier was mixed as an aqueous phase-n-octanol as an organic phase (compared to 1:5), and hydrolyzed at 60°C for 200 min (250 rpm). Add 0.05mol / L glycine solution (v / v1:1) to the extracted organ...
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[0058] Example 3
[0059] The water gardenia was washed and dried, then pulverized through a 40-mesh sieve to obtain water gardenia powder, and then added with water with a solid-to-liquid ratio (w / v) of 1:8, and extracted twice at an ultrasonic power of 120W and a temperature of 35℃. After 1 hour, the extract was passed through a centrifuge (rotating speed: 8000 rpm, 10 min) for solid-liquid separation, and the filtrate was collected to obtain a filter residue. The filtrate was first filtered with an inorganic ceramic microfiltration membrane (MF) with a pore size of 0.5μm, operating temperature 35℃, pressure: 0.5Mpa; the resulting clear liquid (geniposide concentration 1.9mg / mL) was combined with mesoporous molecular sieve SBA-15 The carrier-immobilized β-glucosidase (4.329U / mL, pH5.0) was mixed as the water phase-n-octanol was the organic phase (compared to 1:2), and hydrolyzed at 45°C for 60 min (180 rpm). Add 0.2mol / L glycine solution (v / v2:1) to the extracted organic phase...
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