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Comprehensive extraction method for fructus gardeniae

A technology of comprehensive extraction and water gardenia, applied in chemical instruments and methods, steroids, food science, etc., can solve the problems of complicated process and failure of comprehensive extraction and utilization of active ingredients, and achieve high color value and bright color of pigment , the effect of high yield

Active Publication Date: 2017-02-22
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: In view of the defects in the prior art, such as complicated process and no comprehensive extraction and utilization of active ingredients, the present invention provides a comprehensive extraction method of Gardenia jasminoides

Method used

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  • Comprehensive extraction method for fructus gardeniae

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Wash and dry the water gardenia and crush it through a 40-mesh sieve to obtain the water gardenia powder, then add water with a solid-to-liquid ratio (w / v) of 1:5, and extract once at ultrasonic power 100W and temperature 30°C for 1 hour Finally, the leaching liquid was separated from the solid and liquid through a centrifuge (rotating speed: 8000 rpm, 5 min), and the filtrate was collected to obtain a filter residue. The filtrate was first filtered with an inorganic ceramic microfiltration membrane (MF) with a pore size of 0.5 μm, the operating temperature was 30°C, and the pressure: 0.4Mpa; The immobilized β-glucosidase (0.937U / mL, pH5.0) as the carrier was mixed as the aqueous phase-n-octanol was the organic phase (ratio 1:1), hydrolyzed at 40°C for 40min (100rpm). Add 0.05 mol / L glycine solution (v / v2:1) to the extracted organic phase, and react for 20 minutes in a constant temperature water bath at 80°C to obtain a gardenia blue pigment solution, which is then spra...

Embodiment 2

[0055] After washing and drying the water gardenia, crush it through a 40-mesh sieve to obtain water gardenia powder, then add water with a solid-to-liquid ratio (w / v) of 1:15, and extract 3 times at an ultrasonic power of 200W and a temperature of 50°C. After 1 hour, pass the extract solution through a centrifuge (speed: 15000 rpm, 20 minutes) for solid-liquid separation, collect the filtrate, and obtain a filter residue. The filtrate was first filtered with an inorganic ceramic microfiltration membrane (MF) with a pore size of 0.5 μm, the operating temperature was 60°C, and the pressure: 0.9Mpa; The immobilized β-glucosidase (4.347U / mL, pH5.0) as the carrier was mixed as the aqueous phase-n-octanol was the organic phase (compared to 1:5), hydrolyzed at 60°C for 200min (250rpm). Add 0.05 mol / L glycine solution (v / v1:1) to the extracted organic phase, and react for 55 minutes in a constant temperature water bath at 100°C to obtain a gardenia blue pigment solution, which is con...

Embodiment 3

[0059] Wash and dry Jasmine jasminoides, crush and pass through a 40-mesh sieve to obtain jasmine jasmine powder, then add water with a solid-to-liquid ratio (w / v) of 1:8, and extract twice at an ultrasonic power of 120W and a temperature of 35°C. After 1 hour, pass the extract liquid through a centrifuge (speed: 8000 rpm, 10 min) for solid-liquid separation, collect the filtrate, and obtain a filter residue. The filtrate was first filtered with an inorganic ceramic microfiltration membrane (MF) with a pore size of 0.5 μm, the operating temperature was 35°C, and the pressure: 0.5Mpa; The immobilized β-glucosidase (4.329U / mL, pH5.0) as the carrier was mixed as the aqueous phase-n-octanol was the organic phase (compared to 1:2), hydrolyzed at 45°C for 60min (180rpm). Add 0.2 mol / L glycine solution (v / v2:1) to the extracted organic phase, and react for 40 minutes in a constant temperature water bath at 85°C to obtain a gardenia blue pigment solution, which is concentrated and spr...

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Abstract

The invention discloses a comprehensive extraction method for fructus gardenia. The comprehensive extraction method comprises the following steps: carrying out ultrasonic extraction on the fructus gardenia by taking water as a solvent so as to obtain supernate and solid residues, filtering the supernate by virtue of a micro-filtration membrane to obtain filtrate A containing geniposide and a gardenia yellow pigment, mixing the filtrate A with beta-glucosidase liquid as a water phase, converting geniposide in the filtrate A into genipin by virtue of beta-glucosidase in a two-phase system, and transferring the genipin into the organic phase, namely an organic solvent, so as to react to obtain a gardenia blue pigment; separating and purifying the gardenia yellow pigment in the residual water solution; and extracting the solid residues with ethanol to obtain supernate, and carrying out separation and purification, so as to obtain ursolic acid. The comprehensive extraction method has the beneficial effects that the cost is low, the process is simple and practical, efficient paths for purifying the gardenia yellow pigment and converting the geniposide into the gardenia blue pigment are realized, and the extraction of ursolic acid in the solid residues, the recycling and repeated use of fixed beta-glucosaccharase and the maximum utilization of the fructus gardenia are realized.

Description

technical field [0001] The invention relates to the preparation of natural pigments in the technical fields of plant extraction, biocatalysis, traditional Chinese medicine and food additives, in particular to a comprehensive extraction method of gardenia jasminoides. Background technique [0002] Water gardenia is the dried fruit of Rubiaceae large flower gardenia. Water gardenia and gardenia are similar in shape but larger, the diameter of the fruit is 1.2-1.7cm, the color is brown to brown yellow, the taste is slightly sour and bitter, and the smell is light . There are many chemical components of Gardenia jasminoides, mainly containing a large amount of crocetin, crocetin and its derivatives, and 11 kinds of iridoid glycosides mainly composed of geniposide, as well as chlorogenic acid, ursine Fruit acid, pectin, volatile oil, mannitol, etc., among which the content of pigments is higher than that of gardenia, the extracted pigments are used for food coloring, not only co...

Claims

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Application Information

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IPC IPC(8): C07J63/00C09B61/00C12P17/06C12P1/00A23L5/43
CPCC12P1/00C12P17/06C07J63/00C09B61/00
Inventor 孙芸许任芝姚忠朱本伟
Owner NANJING UNIV OF TECH
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