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Method for biodegradation of microcystins by using microorganisms

A technology of microcystin and biodegradation, applied in chemical instruments and methods, biological water/sewage treatment, bacteria, etc., can solve the problem that MCs are difficult to remove, and achieve the effect of easy cultivation

Inactive Publication Date: 2010-03-31
UNIV OF SCI & TECH BEIJING +1
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for efficient biodegradation and removal of MCs, adopting a strain of Sphingomonas USTB-05 screened from the bottom mud of Dianchi Lake as a bacterial agent or an enzyme preparation prepared after cultivation Fast, safe and efficient biocatalysts are added to water bodies polluted by MCs, so that MCs can be efficiently degraded and removed, and solve the problem that MCs are difficult to remove in water bodies polluted by cyanobacteria blooms.

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  • Method for biodegradation of microcystins by using microorganisms
  • Method for biodegradation of microcystins by using microorganisms
  • Method for biodegradation of microcystins by using microorganisms

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[0020] 1. First prepare the growth medium of Sphingomonas USTB-05, which consists of (per liter): MgSO 7H 2 O 1.0g, KH 2 PO 4 0.5g, K 2 HPO 4 4.0g, NaCl 1.0g, CaCl 2 20mg, FeSO 4 5mg, ZnCl 2 5 mg, MnCl 2 4H 2 O 5mg, CuCl 2 0.5 mg, on this basis, 15.0 g of glucose and 1.5 g of yeast extract powder were added as carbon and nitrogen sources for the growth of Sphingomonas USTB-05. Add 100 milliliters of the prepared liquid medium into a 500 milliliter conical flask, sterilize at high temperature and pressure (121° C.) for 20 minutes, and then sterilize for another 20 minutes under ultraviolet irradiation in a clean bench.

[0021] 2. Under sterile conditions in a clean bench, inoculate 1 ml of Sphingomonas USTB-05 bacteria solution into the liquid culture medium of the Erlenmeyer flask, and conduct batches at a temperature of 30°C and a shaker speed of 200 rpm. After culturing for 3 days, harvest the Sphingomonas USTB-05 cells by pouring off the supernatant after ce...

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Abstract

The invention relates to a method for the biodegradation of microcystins by using microorganisms, which belongs to the field of biotechnology. The method comprises the following steps: screening sphingosine monads USTB-05 of microorganism strains; using a cultured high-cell-density bacterial solution of the sphingosine monads USTB-05 or an enzyme extracted from cells as a biocatalyst; and removingMCs in a water body in an adding mode, or removing the MCs by applying the degradation in a sewage biological treatment process in the presence of the MCs. The method has the characteristics of safety, economy and high efficiency, and has important application values in the aspect of removing MCs pollutions generated by blue alga blooms through degradation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to screening microbial strains (Sphingomonas USTB-05, the English name is Sphingomonas sp.USTB-05, strain preservation number: CGMCC No.3009, preservation date April 13, 2009 Japan) and a method for degrading and removing microcystins (Microcystins, referred to as: MCs) with enzyme preparations. Background technique [0002] In recent years, cyanobacteria blooms have occurred frequently, especially in the three major lakes of Taihu Lake, Dianchi Lake, and Chaohu Lake. Different degrees of cyanobacteria blooms occur every year. One of the biggest hazards of cyanobacterial blooms is the production and release of various algal toxins into water bodies, among which MCs are the most widely produced, most widely distributed, and most harmful types of algal toxins. MCs are a class of monocyclic heptapeptides composed of 7 amino acids. Since the two main variable positions can be replaced with d...

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Application Information

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IPC IPC(8): C02F3/34C12N1/20
Inventor 闫海吴鹏飞王俊峰陈建许阳光
Owner UNIV OF SCI & TECH BEIJING
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